中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2010年
6期
721-724
,共4页
康祖铭%黄生高%凌天牖%沙永红%李春梅%石君
康祖銘%黃生高%凌天牖%沙永紅%李春梅%石君
강조명%황생고%릉천유%사영홍%리춘매%석군
电磁场%牙移动%牙周组织/代谢%血小板源性生长因子/代谢
電磁場%牙移動%牙週組織/代謝%血小闆源性生長因子/代謝
전자장%아이동%아주조직/대사%혈소판원성생장인자/대사
Electromagnetic fields%Tooth movement%Periodontium/ME%Platelet-derived growth factor/ME
目的 探讨旋转脉动磁场对兔实验性牙齿移动过程中牙周组织血小板衍生生长因子A(PDGF-A)表达的影响.方法 30只体重为1.0~1.5 kg的健康新西兰大耳白兔按每组5只随机分为实验1、3、5、7、14、21 d组.2%戊巴比妥钠麻醉下于双侧上颌第一磨牙与切牙间拴结不锈钢螺簧,施力80g,左侧只加力为对照侧,右侧加力加旋转脉动磁场为实验侧,通过免疫组织化学染色进行PDGF-A半定量分析.结果 实验侧牙周组织中PDGF-A表达高于对照侧;实验侧张力区、压力区牙周组织中PDGF-A表达在5、7、14 d时较对照侧差异有统计学意义(5.28±0.14 vs 2.03±0.18,7.63±0.27 vs 2.84±0.12,3.52±0.16 vs 1.65±0.03;8.10±0.13 vs 4.30±0.21,13.27±0.31 vs 6.47±0.15,5.66±0.22 vs 3.15±0.27,P<0.05);在7 d时牙周组织PDGF-A表达达到高峰.结论 旋转脉动磁场照射促进了牙周组织中PDGF-A的表达,促进了牙周组织的改建.
目的 探討鏇轉脈動磁場對兔實驗性牙齒移動過程中牙週組織血小闆衍生生長因子A(PDGF-A)錶達的影響.方法 30隻體重為1.0~1.5 kg的健康新西蘭大耳白兔按每組5隻隨機分為實驗1、3、5、7、14、21 d組.2%戊巴比妥鈉痳醉下于雙側上頜第一磨牙與切牙間拴結不鏽鋼螺簧,施力80g,左側隻加力為對照側,右側加力加鏇轉脈動磁場為實驗側,通過免疫組織化學染色進行PDGF-A半定量分析.結果 實驗側牙週組織中PDGF-A錶達高于對照側;實驗側張力區、壓力區牙週組織中PDGF-A錶達在5、7、14 d時較對照側差異有統計學意義(5.28±0.14 vs 2.03±0.18,7.63±0.27 vs 2.84±0.12,3.52±0.16 vs 1.65±0.03;8.10±0.13 vs 4.30±0.21,13.27±0.31 vs 6.47±0.15,5.66±0.22 vs 3.15±0.27,P<0.05);在7 d時牙週組織PDGF-A錶達達到高峰.結論 鏇轉脈動磁場照射促進瞭牙週組織中PDGF-A的錶達,促進瞭牙週組織的改建.
목적 탐토선전맥동자장대토실험성아치이동과정중아주조직혈소판연생생장인자A(PDGF-A)표체적영향.방법 30지체중위1.0~1.5 kg적건강신서란대이백토안매조5지수궤분위실험1、3、5、7、14、21 d조.2%무파비타납마취하우쌍측상합제일마아여절아간전결불수강라황,시력80g,좌측지가력위대조측,우측가력가선전맥동자장위실험측,통과면역조직화학염색진행PDGF-A반정량분석.결과 실험측아주조직중PDGF-A표체고우대조측;실험측장력구、압력구아주조직중PDGF-A표체재5、7、14 d시교대조측차이유통계학의의(5.28±0.14 vs 2.03±0.18,7.63±0.27 vs 2.84±0.12,3.52±0.16 vs 1.65±0.03;8.10±0.13 vs 4.30±0.21,13.27±0.31 vs 6.47±0.15,5.66±0.22 vs 3.15±0.27,P<0.05);재7 d시아주조직PDGF-A표체체도고봉.결론 선전맥동자장조사촉진료아주조직중PDGF-A적표체,촉진료아주조직적개건.
Objective To investigate the effects of rotating pulsed magnetic field on platelet derived growth factor a (PDGF-A) expression in periodontal tissue during tooth movement. Methods 30 white rabbits were random divided into 6 groups with 5 rabbits each group,including groups of 1,3,5,7,14 and 21 days. Under anesthesia condition by 2% pentobarbital sodium,the stainless coil springs were fixed between the first maxillary molar and the incisor producing the force of 80g. The experimental group was treated by rotating pulsed magnetic field and the force, the control group was only treated by t he force. The expression of PDGF-A was half-quantitatively investigated through immunohistochemical analysis. Results The expression of PDGF-AA in the experimental group enhanced apparently compared with that in the control group. There were significant differences among the 5,7, and 14day groups (5. 28 ± 0. 14 vs 2. 03 ±0. 18,7.63±0.27 vs 2. 84 ±0. 12,3.52 ±0. 16 vs 1.65 ±0.03;8.10±0.13 vs 4. 30 ±0. 21,13. 27 ±0. 31 vs 6.47 ± 0.15,5.66 ± 0.22 vs 3. 15 ± 0. 27, P < 0. 05). The expression of PDGF-AA of 7 day group was higher than that of other groups. Conclusion Rotating pulsed magnetic field promotes the expression of PDGF-A in the periodontal tissue and alveolar bone remodeling.