中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2011年
11期
1023-1028
,共6页
逄宇%夏辉%姜广路%刘冠%梁倩%周杨%王胜芬%尚媛媛%赵冰%宋媛媛%赵雁林
逄宇%夏輝%薑廣路%劉冠%樑倩%週楊%王勝芬%尚媛媛%趙冰%宋媛媛%趙雁林
방우%하휘%강엄로%류관%량천%주양%왕성분%상원원%조빙%송원원%조안림
分枝杆菌,结核%寡核苷酸类%基因型%抗药性,细菌
分枝桿菌,結覈%寡覈苷痠類%基因型%抗藥性,細菌
분지간균,결핵%과핵감산류%기인형%항약성,세균
Mycobacterium tuberculosis%Oligonucleotides%Genotype%Drug resistance,bacterial
目的 选用间隔区寡核苷酸分型方法对有中国代表性的菌株进行基因分型,研究不同基因型在中国的流行情况,并分析基因型与耐药表型的关系.方法 中国疾病预防控制中心于2007-2008年,按照流行病学抽样的原则从中国31个省收集涂片阳性的结核病患者的临床分离株4 017株,采用比例法进行药物敏感性实验,选用寡核苷酸分型方法对菌株进行基因分型.基因型检出率差异比较采用x2检验.结果 在4017株结核分枝杆菌临床分离株中,北京基因型菌株包括2 500株(62.2％).北方地区来源菌株北京基因型检出率达76.5％(1 913株),高于南方地区检出率(53.2％,1 330株),差异有统计学意义(x2=219.69,P＜0.05).南方地区T1型检出率达13.3％(332株),高于北方地区检出率(4.3％,108株),差异有统计学意义(x2=88.07,P＜0.05).北京基因型在耐利福平(21.7％)、耐氧氟沙星(4.9％)和MDR( 11.3％)的比例都高于非北京基因型的相应菌株比例18.4％、2.4％和7.4％,差异有统计学意义(x2值分别为22.10、14.42和14.83,P均＜0.05).结论 北京基因型目前仍然是中国主要流行基因型,并且比例呈现出地域差别,北方地区显著高于南方地区.北京基因型菌株与耐利福平、耐氧氟沙星和MDR有关.
目的 選用間隔區寡覈苷痠分型方法對有中國代錶性的菌株進行基因分型,研究不同基因型在中國的流行情況,併分析基因型與耐藥錶型的關繫.方法 中國疾病預防控製中心于2007-2008年,按照流行病學抽樣的原則從中國31箇省收集塗片暘性的結覈病患者的臨床分離株4 017株,採用比例法進行藥物敏感性實驗,選用寡覈苷痠分型方法對菌株進行基因分型.基因型檢齣率差異比較採用x2檢驗.結果 在4017株結覈分枝桿菌臨床分離株中,北京基因型菌株包括2 500株(62.2％).北方地區來源菌株北京基因型檢齣率達76.5％(1 913株),高于南方地區檢齣率(53.2％,1 330株),差異有統計學意義(x2=219.69,P＜0.05).南方地區T1型檢齣率達13.3％(332株),高于北方地區檢齣率(4.3％,108株),差異有統計學意義(x2=88.07,P＜0.05).北京基因型在耐利福平(21.7％)、耐氧氟沙星(4.9％)和MDR( 11.3％)的比例都高于非北京基因型的相應菌株比例18.4％、2.4％和7.4％,差異有統計學意義(x2值分彆為22.10、14.42和14.83,P均＜0.05).結論 北京基因型目前仍然是中國主要流行基因型,併且比例呈現齣地域差彆,北方地區顯著高于南方地區.北京基因型菌株與耐利福平、耐氧氟沙星和MDR有關.
목적 선용간격구과핵감산분형방법대유중국대표성적균주진행기인분형,연구불동기인형재중국적류행정황,병분석기인형여내약표형적관계.방법 중국질병예방공제중심우2007-2008년,안조류행병학추양적원칙종중국31개성수집도편양성적결핵병환자적림상분리주4 017주,채용비례법진행약물민감성실험,선용과핵감산분형방법대균주진행기인분형.기인형검출솔차이비교채용x2검험.결과 재4017주결핵분지간균림상분리주중,북경기인형균주포괄2 500주(62.2％).북방지구래원균주북경기인형검출솔체76.5％(1 913주),고우남방지구검출솔(53.2％,1 330주),차이유통계학의의(x2=219.69,P＜0.05).남방지구T1형검출솔체13.3％(332주),고우북방지구검출솔(4.3％,108주),차이유통계학의의(x2=88.07,P＜0.05).북경기인형재내리복평(21.7％)、내양불사성(4.9％)화MDR( 11.3％)적비례도고우비북경기인형적상응균주비례18.4％、2.4％화7.4％,차이유통계학의의(x2치분별위22.10、14.42화14.83,P균＜0.05).결론 북경기인형목전잉연시중국주요류행기인형,병차비례정현출지역차별,북방지구현저고우남방지구.북경기인형균주여내리복평、내양불사성화MDR유관.
Objective To study the genotypes of representative Mycobacterium tuberculosis (M.tuberculosis) strains from China with spacer oligonucleotide typing (spoligotyping),and to investigate the prevalence of different genotypes TB in China,and analyse the relationship between genotype and drug resistance.Methods 4017 clinical isolates were collected by Chinese Center for Disease Control and Prevention from 2007 to 2008 in 31 provinces in China according to sampling principle of epidemiology.Drug susceptibility testing was performed using proportion method,and spoligotyping was chosen to carry out genotyping of these M.tuberculosis.In addition,chi-square test was used to compare the differences among the detection rate of different genotypes.Results Among the 4017 M.tuberculosis isolates,2500 ( 62.2％ ) isolates belonged to Beijing genotype.The percentage of Beijing genotypes in the northern of China was higher than that in the southern of China ( 76.5％ vs.53.2％,x2 =219.69,P ＜ 0.05 ),while T1 genotypes were more common in the southern China,compared with that in northern China ( 13.3％ vs.4.3％,x2 =219.69,P ＜ 0.05 ).The differences were statistically significant.The proportions of Rifampinresistant (21.7％ vs.21.7％ ),Ofloxacin-resistant (4.9％ vs.2.4％ ) and Multidrug-resistant ( 11.3％vs.7.4％ ) isolates among Beijing genotype strains were significantly higher than those among non-Beijing strains (x2 =22.10,14.42 and 14.83,respectively,P ＜ 0.05 ).Conclusions Beijing genotype was still predominant epidemic genotypes.The percentage of Beijing genotype showed difference between distinct areas,and the percentage of Beijing genotypes in northern China was higher than that in southern China.Beijing genotype strains reveal correlation with Rifampin-resistance,Ofloxacin-resistance and Multidrug-resistance.
