中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2006年
27期
190-192
,共3页
陈正跃%黄丽君%许建文%张光军%邱培勇%张积霞%王永学
陳正躍%黃麗君%許建文%張光軍%邱培勇%張積霞%王永學
진정약%황려군%허건문%장광군%구배용%장적하%왕영학
衰老%美容%鸡%超氧化物歧化酶%丙二醛%脂褐质
衰老%美容%鷄%超氧化物歧化酶%丙二醛%脂褐質
쇠로%미용%계%초양화물기화매%병이철%지갈질
背景:鸡胚胎具有补肾填精、益气健脾、补血养血、美容养颜、提高机体整体体质的作用.目的:观察鸡胚胎素对动物模型某些衰老指标、血细胞数量及免疫器官质量的影响,分析其抗衰老、美容及免疫调节作用.设计:随机对照观察.单位:新乡医学院药物研究室.材料:实验于1998-04/2002-09在新乡医学院药物研究室完成,选择昆明小鼠80只,雌雄各半.方法:①鸡胚胎素对衰老小鼠血清超氧化物歧化酶活性、丙二醛含量、肝脏脂褐素含量及尾腱中羟脯氨酸含量的影响:取30只小鼠,按随机排列表法分为3组,即鸡胚胎素组、D-半乳糖模型组和正常对照组,每组10只.前两组注射D-半乳糖复制衰老模型,鸡胚胎素组小鼠灌服鸡胚胎素,测定各组小鼠血清超氧化物歧化酶活性、丙二醛含量、肝脏脂褐素含量(衰老指标)及尾腱中羟脯氨酸含量(皮肤弹性指标).②鸡胚胎素对免疫功能低下小鼠免疫器官质量的影响:取30只小鼠,分组方法同上.鸡胚胎素组小鼠灌服鸡胚胎素,鸡胚胎素组、环磷酰胺模型组小鼠腹腔注射环磷酰胺复制免疫抑制模型,计算胸腺(脾)指数(mg/g)=胸腺(脾)质量/体质量.③鸡胚胎素对血虚小鼠红细胞及血红蛋白数量的影响:取20只小鼠,按随机排列表法分为2组,即鸡胚胎素组和正常对照组,每组10只.测各组小鼠给药前血红细胞和血红蛋白值,应用尾部放血法复制失血性血虚模型后,分别灌胃鸡胚胎素和生理盐水,尾部采血测红细胞和血红蛋白值.主要观察指标:小鼠血清超氧化物歧化酶活性、丙二醛含量、肝脏脂褐素含量、尾腱中羟脯氨酸含量、胸腺指数、脾指数、血红细胞及血红蛋白含量.结果:80只小鼠全部进入结果分析,无脱失.①鸡胚胎素组小鼠的血清超氧化物歧化酶活性及尾腱中羟脯氨酸含量均显著高于D-半乳糖模型组(P＜0.01);鸡胚胎素组小鼠的血清丙二醛含量及肝脏脂褐素含量均显著低于D-半乳糖模型组(P＜0.01).②鸡胚胎素组小鼠的胸腺、脾指数均显著高于环磷酰胺模型组(P＜0.05～0.01).③鸡胚胎素组小鼠的血红细胞和血红蛋白增值均显著高于正常对照组(P＜0.01).结论:口服鸡胚胎素具有提高机体抗氧化能力的作用,能显著增加皮肤弹性,对于环磷酰胺造成的免疫器官质量及其免疫功能下降具有明显的改善作用,并且具有较好的补血作用.
揹景:鷄胚胎具有補腎填精、益氣健脾、補血養血、美容養顏、提高機體整體體質的作用.目的:觀察鷄胚胎素對動物模型某些衰老指標、血細胞數量及免疫器官質量的影響,分析其抗衰老、美容及免疫調節作用.設計:隨機對照觀察.單位:新鄉醫學院藥物研究室.材料:實驗于1998-04/2002-09在新鄉醫學院藥物研究室完成,選擇昆明小鼠80隻,雌雄各半.方法:①鷄胚胎素對衰老小鼠血清超氧化物歧化酶活性、丙二醛含量、肝髒脂褐素含量及尾腱中羥脯氨痠含量的影響:取30隻小鼠,按隨機排列錶法分為3組,即鷄胚胎素組、D-半乳糖模型組和正常對照組,每組10隻.前兩組註射D-半乳糖複製衰老模型,鷄胚胎素組小鼠灌服鷄胚胎素,測定各組小鼠血清超氧化物歧化酶活性、丙二醛含量、肝髒脂褐素含量(衰老指標)及尾腱中羥脯氨痠含量(皮膚彈性指標).②鷄胚胎素對免疫功能低下小鼠免疫器官質量的影響:取30隻小鼠,分組方法同上.鷄胚胎素組小鼠灌服鷄胚胎素,鷄胚胎素組、環燐酰胺模型組小鼠腹腔註射環燐酰胺複製免疫抑製模型,計算胸腺(脾)指數(mg/g)=胸腺(脾)質量/體質量.③鷄胚胎素對血虛小鼠紅細胞及血紅蛋白數量的影響:取20隻小鼠,按隨機排列錶法分為2組,即鷄胚胎素組和正常對照組,每組10隻.測各組小鼠給藥前血紅細胞和血紅蛋白值,應用尾部放血法複製失血性血虛模型後,分彆灌胃鷄胚胎素和生理鹽水,尾部採血測紅細胞和血紅蛋白值.主要觀察指標:小鼠血清超氧化物歧化酶活性、丙二醛含量、肝髒脂褐素含量、尾腱中羥脯氨痠含量、胸腺指數、脾指數、血紅細胞及血紅蛋白含量.結果:80隻小鼠全部進入結果分析,無脫失.①鷄胚胎素組小鼠的血清超氧化物歧化酶活性及尾腱中羥脯氨痠含量均顯著高于D-半乳糖模型組(P＜0.01);鷄胚胎素組小鼠的血清丙二醛含量及肝髒脂褐素含量均顯著低于D-半乳糖模型組(P＜0.01).②鷄胚胎素組小鼠的胸腺、脾指數均顯著高于環燐酰胺模型組(P＜0.05～0.01).③鷄胚胎素組小鼠的血紅細胞和血紅蛋白增值均顯著高于正常對照組(P＜0.01).結論:口服鷄胚胎素具有提高機體抗氧化能力的作用,能顯著增加皮膚彈性,對于環燐酰胺造成的免疫器官質量及其免疫功能下降具有明顯的改善作用,併且具有較好的補血作用.
배경:계배태구유보신전정、익기건비、보혈양혈、미용양안、제고궤체정체체질적작용.목적:관찰계배태소대동물모형모사쇠로지표、혈세포수량급면역기관질량적영향,분석기항쇠로、미용급면역조절작용.설계:수궤대조관찰.단위:신향의학원약물연구실.재료:실험우1998-04/2002-09재신향의학원약물연구실완성,선택곤명소서80지,자웅각반.방법:①계배태소대쇠로소서혈청초양화물기화매활성、병이철함량、간장지갈소함량급미건중간포안산함량적영향:취30지소서,안수궤배렬표법분위3조,즉계배태소조、D-반유당모형조화정상대조조,매조10지.전량조주사D-반유당복제쇠로모형,계배태소조소서관복계배태소,측정각조소서혈청초양화물기화매활성、병이철함량、간장지갈소함량(쇠로지표)급미건중간포안산함량(피부탄성지표).②계배태소대면역공능저하소서면역기관질량적영향:취30지소서,분조방법동상.계배태소조소서관복계배태소,계배태소조、배린선알모형조소서복강주사배린선알복제면역억제모형,계산흉선(비)지수(mg/g)=흉선(비)질량/체질량.③계배태소대혈허소서홍세포급혈홍단백수량적영향:취20지소서,안수궤배렬표법분위2조,즉계배태소조화정상대조조,매조10지.측각조소서급약전혈홍세포화혈홍단백치,응용미부방혈법복제실혈성혈허모형후,분별관위계배태소화생리염수,미부채혈측홍세포화혈홍단백치.주요관찰지표:소서혈청초양화물기화매활성、병이철함량、간장지갈소함량、미건중간포안산함량、흉선지수、비지수、혈홍세포급혈홍단백함량.결과:80지소서전부진입결과분석,무탈실.①계배태소조소서적혈청초양화물기화매활성급미건중간포안산함량균현저고우D-반유당모형조(P＜0.01);계배태소조소서적혈청병이철함량급간장지갈소함량균현저저우D-반유당모형조(P＜0.01).②계배태소조소서적흉선、비지수균현저고우배린선알모형조(P＜0.05～0.01).③계배태소조소서적혈홍세포화혈홍단백증치균현저고우정상대조조(P＜0.01).결론:구복계배태소구유제고궤체항양화능력적작용,능현저증가피부탄성,대우배린선알조성적면역기관질량급기면역공능하강구유명현적개선작용,병차구유교호적보혈작용.
BACKGROUND: Eggembryosin is characterized by tonifying kidney and vital essence, invigorating qi and spleen, replenishing and activating blood, beautifying and nourishing face, and improving constitution of whole organism.OBJECTIVE: To observe the influence of eggembryosin on aging index,quantity of hemocytes and quality of immune organs of animal models, and analyze its effect on cosmetology, anti-senility and immunoloregulation.DESIGN: Randomized controlled observation.SETTING: Department of Pharmacology, Xinxiang Medical College.MATERIALS: The experiment was carried out in the Department of Pharmacology of Xinxiang Medical College between April 1998 and September 2002. A total of 80 Kunming mice of both sexes were selected in this study.METHODS: ① Effect of eggembryosin on activity of superoxide dismutase (SOD) in serum, content of malondialdehyde (MDA), lipofuscin and hydroxyproline respectively in serum, liver and tendon of tail of aging mice: Thirty mice were divided into 3 groups according to randomly lined table, including eggembryosin group, D-galactose model group and normal control group with 10 in each group. The former two groups were injected with D-galactose to copy aging models, and eggembryosin group were perfused with eggembryosin to measure activity of SOD in serum, content of MDA, lipofuscin (aging index) and hydroxyproline (elasric index of skin) respectively in serum, liver and tendon of tail. ② Effect of eggembryosin on mass of immune organs of mice with low immunological function: Thirty mice were selected and divided as the same way mentioned above. Mice in eggembryosin group were perfused with eggembryosin. In addition, those in eggembryosin group and cyclophosphamide (CAP) group were peritoneally injected with CAP to copy immunosuppression models and calculate index of thymus (spleen) (mg/g) = weight of thymus (spleen)/body. ③ Effect of eggembryosin on quantities of erythrocytes and hemoglobin of mice with blood deficiency: Twenty mice were divided into 2 groups according to randomly lined table, including eggembryosin group and normal control group with 10 in each group. Values of erythrocytes and hemoglobins of mice were measured before administration. Models in types of blood-deficiency blood-loss were copied with tail xsanguinations, and then, mice were perfused with eggembryosin and saline, respectively. Blood of mice were collected from their tails to measure values of erythrocytes and hemoglobin.MAIN OUTCOME MEASURES: Activity of superoxide dismutase (SOD) in serum, content of malondialdehyde (MDA), lipofuscin and hydroxyproline respectively in serum, liver and tendon of tail of aging mice, index of thymus, and spleen, contents of blood erythrocytes and hemoglobins.RESULTS: A total of 80 mice were involved in the final analysis without any loss. ① Activity of SOD in serum and contents of hydroxyproline in tendon of tail of mice in eggembryosin group were significantly higher than those of mice in D-galactose model group (P ＜ 0.01); content of MDA in serum and content of lipofuscin in liver of mice in eggembryosin group were significantly lower than those of mice in D-galactose model group (P＜ 0.01). ② Indexes of thymus and spleen of mice in eggembryosin group were higher than those of mice in CAP group (P ＜ 0.05-0.01). ③ Increasing values of blood erythrocytes and hemoglobin of mice in eggembryosin group were significantly higher than those of mice in normal control group (P ＜ 0.01).CONCLUSION: Eggembryosin taken orally can improveanti-oxidation of organism and elasticity of skin, increase quality of immune organs damaged by CAP and immunological function, and nourish the blood.
