CAJ | 학술논문

目的检测喉癌组织及手术切缘中Survivin、COX-2表达情况,探讨两者的表达与术后局部复发的关系.方法选择62例原发灶为鳞状细胞癌、手术切缘组织病理学肿瘤阴性者,对喉癌组织及切缘连续切片,行HE染色,以免疫组化法分别进行Survivin及 COX-2检测.结果 62例喉癌患者原发灶组织中Survivin(+)48例及COX-2(+)38例的复发率分别为33.3% (16/48)和26.3%(10/38)高于阴性者14.3% (2/14)和12.5%(3/24),差异有统计学意义(P<0.05);手术切缘中Survivin(+)27例及COX-2(+)19例的复发率分别为29.6% (5/27)和26.3%(5/19)高于阴性者11.4%(3/35)和4.7%(2/43),差异有统计学意义(P<0.05).喉癌组织中Survivin及COX-2表达同时异常,且手术切缘组织Survivin及COX-2表达也同时异常,术后复发率为100%(5/5).结论 Survivin及COX-2检测可以作为预测喉癌预后的生物学标志,在病理学阴性手术切缘组织中两者联合检测对于判断术后局部复发有更大的意义.
목적 검측후암조직급수술절연중Survivin、COX-2표체정황,탐토량자적표체여술후국부복발적관계.방법 선택62례원발조위린상세포암、수술절연조직병이학종류음성자,대후암조직급절연련속절편,행HE염색,이면역조화법분별진행Survivin급 COX-2검측.결과 62례후암환자원발조조직중Survivin(+)48례급COX-2(+)38례적복발솔분별위33.3% (16/48)화26.3%(10/38)고우음성자14.3% (2/14)화12.5%(3/24),차이유통계학의의(P<0.05);수술절연중Survivin(+)27례급COX-2(+)19례적복발솔분별위29.6% (5/27)화26.3%(5/19)고우음성자11.4%(3/35)화4.7%(2/43),차이유통계학의의(P<0.05).후암조직중Survivin급COX-2표체동시이상,차수술절연조직Survivin급COX-2표체야동시이상,술후복발솔위100%(5/5).결론 Survivin급COX-2검측가이작위예측후암예후적생물학표지,재병이학음성수술절연조직중량자연합검측대우판단술후국부복발유경대적의의.
Objective To explore the relationship of the local recurrence with the expression of protein Survivin and COX-2 in the primary lesions and the surgical margins of laryngeal carcinoma.Methods The primary lesions and the surgical margins of laryngeal carcinoma of 62 patients were made into serial sections. Immunochemical method (HE staining) was used to detect the expression of Survivin and COX-2 protein.Results In these 62 cases of patients, the recurrent rates of the primary lesions positive for Survivin(48 cases) and COX-2(38 cases) were 33.3% (16/48) and 26.3%(10/38)respectively, which were significantly higher than those negative for them14.3% (2/14) and 12.5%(3/24)(P<0.05);the recurrent rates of surgical margins positive for Survivin (27 cases) and COX-2(19 cases) were 29.6% (5/27) and 26.3% (5/19) respectively, which were significantly higher than those negative for them11.4%(3/35) and 4.7%(2/43)(P<0.05). The expression of Survivin and COX-2 proteins in the primary lesions and surgical margins was abnormal; the recurrent rate of the laryngeal carcinoma was 100% (5/5) after surgery.Conclusion Survivin and COX-2 protein can be used as biomarkers for local recurrence of laryngeal carcinoma after operation. Detection of Survivin and COX-2 in both the primary lesions and the surgical margins may be of greater significance in forecasting local recurrence.