CAJ | 학술논문

目的研究大鼠视神经部分损伤后视网膜基质细胞衍生因子-1α(SDF-1α)的表达变化.方法制作大鼠视神经部分损伤模型,于损伤后1、2、3、5、7、10、14 d收集视网膜组织,采用酶联免疫吸附法和实时荧光定量PCR分别测定损伤组(n=28)、假手术组(n=28)和正常对照组(n=12)大鼠视网膜组织中的SDF-1α蛋白及mRNA的表达.结果视神经损伤后各时间点,损伤组视网膜组织中SDF-1α蛋白和mRNA的表达较假手术组及正常对照组均明显升高(P<0.01),均在损伤后第5天出现峰值.伤后14 d,损伤组视网膜组织中SDF-1α蛋白及mRNA仍维持在高表达状态.结论视神经部分损伤后SDF-1α在视网膜中的表达出现上调,并可在较长时间内维持高表达.
목적 연구대서시신경부분손상후시망막기질세포연생인자-1α(SDF-1α)적표체변화.방법 제작대서시신경부분손상모형,우손상후1、2、3、5、7、10、14 d수집시망막조직,채용매련면역흡부법화실시형광정량PCR분별측정손상조(n=28)、가수술조(n=28)화정상대조조(n=12)대서시망막조직중적SDF-1α단백급mRNA적표체.결과 시신경손상후각시간점,손상조시망막조직중SDF-1α단백화mRNA적표체교가수술조급정상대조조균명현승고(P<0.01),균재손상후제5천출현봉치.상후14 d,손상조시망막조직중SDF-1α단백급mRNA잉유지재고표체상태.결론 시신경부분손상후SDF-1α재시망막중적표체출현상조,병가재교장시간내유지고표체.
Objective To investigate the changes of expression of stromal cell-derived factor 1α (SDF-1α) in retinas after partial optic nerve injury in rats. Methods Models with injury of partial optic nerve were induced in rats. Retinal tissues were collected 1,2,3,5,7,10 and 14 d after injury. Enzyme linked immunosorbent assay and Real-time quantitative PCR were employed to detect the expression of SDF-1α protein and mRNA in retinal tissues respectively in injury group (n=28), sham operated group (n=28) and normal control group (n=12). Results The expression of SDF-1α protein and mRNA in retinas was higher than that in sham operated group and normal control group at different time points after injury (P<0.01), and it reached the peak at the 5th day after injury. The expression of SDF-1α protein and mRNA maintained a high level at the 14th day after injury. Conclusion The expression of SDF-1α protein and mRNA is up-regulated after partial optic nerve injury, and may last for a long time.