CAJ | 학술논문

该文围绕目前实验室制备11S和7S组分的分离技术成果,结合作者所在实验室的分离技术(微小毛霉法),比较了各种分离方法的差异.早期分离提取技术利用的原理多为"碱溶酸提"和"冷沉"作用,之后不断辅以其它物理或生物技术进行改进,以提高各蛋白组分的回收率和纯度.其中,Thanh法首次完整地提出了大豆蛋白的组分分离方法,而Nango法和Wu法的引用次数较多,Deak法采用的Ca2+沉淀的方法效果则是实验室分离方法中的最优方法.
해문위요목전실험실제비11S화7S조분적분리기술성과,결합작자소재실험실적분리기술(미소모매법),비교료각충분리방법적차이.조기분리제취기술이용적원리다위"감용산제"화"랭침"작용,지후불단보이기타물리혹생물기술진행개진,이제고각단백조분적회수솔화순도.기중,Thanh법수차완정지제출료대두단백적조분분리방법,이Nango법화Wu법적인용차수교다,Deak법채용적Ca2+침정적방법효과칙시실험실분리방법중적최우방법.
Combining with our laboratory's isolation techniques (application of Mucor pusillus), this article compared different separation methods of 11S and 7S fractions in laboratory-scale. The principles of most separation techniques in early period were mainly focus on "alkali extraction and acid precipitation" and "cold precipitation" effects, and then other physical or biological technologies were continuously supplemented for the purpose of improving the yield and purity of protein components. Among them, Thanh's method was the first to realize the contemporary separation technique of soy protein components. Nagano's method and Wu's method were the classical methods quoted for many times. And the Deak's method got the best isolation efficiency by using Ca2+ as a precipitating agent.