中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2009年
2期
119-122
,共4页
史宏灿%裴昶%徐洪%邓卫军
史宏燦%裴昶%徐洪%鄧衛軍
사굉찬%배창%서홍%산위군
气管%人工器官%生物相容性材料%动物实验%溶血
氣管%人工器官%生物相容性材料%動物實驗%溶血
기관%인공기관%생물상용성재료%동물실험%용혈
Trachea%Artificial organs%Bioeompatible materials%Animal experimentation%Hemolysis
目的 对制作人工气管的相关组分材料进行生物相容性评价.方法 皮肤致敏实验:雄性豚鼠分5组(n=8),纳米碳纤维组、胶原蛋白-羟基磷灰石组、混合材料组、阳性对照组(甲醛)和阴性对照组(生理盐水),观察各组的材料浸渍液是否引起豚鼠皮肤过敏反应.热原实验:新西兰兔分4组(n=3),纳米碳纤维组、胶原蛋白-羟基磷灰石组、混合材料组和阴性对照组(生理盐水),观察各组的材料浸渍液是否含有热原物质.以及通过溶血实验观察各组的材料浸渍液对兔血细胞溶血率的影响.结果 本实验涉及的生物材料致敏率均为0,与阴性对照组的皮肤致敏反应差异均无统计学意义(P>0.05).各材料组动物注射材料浸渍液后体温升高分别为(0.3±0.2)、(0.2±0.1)、(0.3±0.2)℃,均在0.6℃以下,且升高总数均在1.4℃以下.各组浸渍液溶血率分别为(0.130 1±0.000 4)%、(0.200 4±0.000 4)%、(0.150 2±0.000 4)%,均<5%.结论 人工气管的组分材料均符合生物相容性评价实验的安全标准,可为建立进一步动物实验模型提供相关依据.
目的 對製作人工氣管的相關組分材料進行生物相容性評價.方法 皮膚緻敏實驗:雄性豚鼠分5組(n=8),納米碳纖維組、膠原蛋白-羥基燐灰石組、混閤材料組、暘性對照組(甲醛)和陰性對照組(生理鹽水),觀察各組的材料浸漬液是否引起豚鼠皮膚過敏反應.熱原實驗:新西蘭兔分4組(n=3),納米碳纖維組、膠原蛋白-羥基燐灰石組、混閤材料組和陰性對照組(生理鹽水),觀察各組的材料浸漬液是否含有熱原物質.以及通過溶血實驗觀察各組的材料浸漬液對兔血細胞溶血率的影響.結果 本實驗涉及的生物材料緻敏率均為0,與陰性對照組的皮膚緻敏反應差異均無統計學意義(P>0.05).各材料組動物註射材料浸漬液後體溫升高分彆為(0.3±0.2)、(0.2±0.1)、(0.3±0.2)℃,均在0.6℃以下,且升高總數均在1.4℃以下.各組浸漬液溶血率分彆為(0.130 1±0.000 4)%、(0.200 4±0.000 4)%、(0.150 2±0.000 4)%,均<5%.結論 人工氣管的組分材料均符閤生物相容性評價實驗的安全標準,可為建立進一步動物實驗模型提供相關依據.
목적 대제작인공기관적상관조분재료진행생물상용성평개.방법 피부치민실험:웅성돈서분5조(n=8),납미탄섬유조、효원단백-간기린회석조、혼합재료조、양성대조조(갑철)화음성대조조(생리염수),관찰각조적재료침지액시부인기돈서피부과민반응.열원실험:신서란토분4조(n=3),납미탄섬유조、효원단백-간기린회석조、혼합재료조화음성대조조(생리염수),관찰각조적재료침지액시부함유열원물질.이급통과용혈실험관찰각조적재료침지액대토혈세포용혈솔적영향.결과 본실험섭급적생물재료치민솔균위0,여음성대조조적피부치민반응차이균무통계학의의(P>0.05).각재료조동물주사재료침지액후체온승고분별위(0.3±0.2)、(0.2±0.1)、(0.3±0.2)℃,균재0.6℃이하,차승고총수균재1.4℃이하.각조침지액용혈솔분별위(0.130 1±0.000 4)%、(0.200 4±0.000 4)%、(0.150 2±0.000 4)%,균<5%.결론 인공기관적조분재료균부합생물상용성평개실험적안전표준,가위건립진일보동물실험모형제공상관의거.
Objective To evaluate the biocompatibility of the composite materials for making artificial airtube. Methods Skin hypersusceptibility test: 40 male guinea pigs were divided into 5 groups (n= 8), including nano-carbon fibers group, collagen protein/nano- hydroxyapatite (HAC) group, mixed materials group, positive control (formaldehyde) group and negative control (normal saline) group. Skin allergic response induced by the leaching liquor of each material was observed. Pyrogen test: 12 New Zealand rabbits were divided into 4 groups (n=3), including nano-carbon fibers group, collagen protein/nano-HAC group, mixed materials group and negative control (normal saline) group. Pyrogen in the leaching liquor of each material was examined. Hemolysis test was used to investigate the effect of the leaching liquor of each material on rabbit' s blood cells hemolysis rate. Results Sensibilization rate of each material was 0. There was no significant difference in statistics compared with negative control group(P>0.05 ). In pyrogen test, the fervescence of the animals in each group were (0.3±0.2), (0.2±0.1) and (0.3±0.2) ℃, respectively (all less than 0.6℃ ), and the sum total of fervescence was less than 1.4℃. The hemolysis rates of all groups were (0.130 1±0.000 4)%, (0.200 4±0.000 4)% and(0.150 2±0.000 4)% (all<5%). Conclusions Consistent materials for new artificial airtube are conformed to safety standard by biocompatibility test. The results provide evidences to further study of animal experimental model establishment.
