中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2009年
1期
34-38
,共5页
涂文勇%刘璐%陈志强%赵红%文剑
塗文勇%劉璐%陳誌彊%趙紅%文劍
도문용%류로%진지강%조홍%문검
极低频磁场%肝癌细胞株BEL-7402%凋亡%基因芯片
極低頻磁場%肝癌細胞株BEL-7402%凋亡%基因芯片
겁저빈자장%간암세포주BEL-7402%조망%기인심편
Extremely low frequency electromagnetic field%Liver carcinoma cell lines BEL-7402%Apoptosis%Gene microarray
目的 研究极低频磁场联合x线对人肝癌细胞株BEL-7402的作用机制.方法 人肝癌细胞株BEL-7402经0、2、4、6、8、10 Gy X线照射后,用极低频磁场处理(100 Hz、0.7 mT、30 min、3 d)后,利用扫描电镜作形态观察,流式细胞仪、基因芯片研究细胞凋亡机制.结果 扫描电镜形态显示,极低频磁场联合X线组肝癌细胞株BEL-7402发生凋亡改变.流式细胞仪观察结果显示,当X线剂量是2、4,6、8、10 Gy,极低频磁场联合x线凋亡率为10.0%、14.5%、4.3%、5.1%、7.1%,X线组凋亡率是0.1%、8.10%、0.1%、0.4%、2.2%(P<0.05).基因芯片结果显示,极低频磁场联合X线组共有1465条出现上调,1108条出现下调.其中比值≥2倍的基因共有336条,上调262条,下调74条.与凋亡相关基因共110条,上调基因71条,下调基因39条.涉及CDC25、CHKl、ATM、p38、PTEN、p53、G1/S、Fas、G2/M、Cell Cycle、Apoptosis、Caspase基因通路中的基因.极低频磁场加X线组差异表达结果中有13条同时出现在极低频磁场组.42条同时出现在X线组.结论 极低频磁场与X线通过不同的基因通路影响细胞凋亡.极低频磁场与X线对人肝癌细胞株BEL-7402凋亡有协同作用.
目的 研究極低頻磁場聯閤x線對人肝癌細胞株BEL-7402的作用機製.方法 人肝癌細胞株BEL-7402經0、2、4、6、8、10 Gy X線照射後,用極低頻磁場處理(100 Hz、0.7 mT、30 min、3 d)後,利用掃描電鏡作形態觀察,流式細胞儀、基因芯片研究細胞凋亡機製.結果 掃描電鏡形態顯示,極低頻磁場聯閤X線組肝癌細胞株BEL-7402髮生凋亡改變.流式細胞儀觀察結果顯示,噹X線劑量是2、4,6、8、10 Gy,極低頻磁場聯閤x線凋亡率為10.0%、14.5%、4.3%、5.1%、7.1%,X線組凋亡率是0.1%、8.10%、0.1%、0.4%、2.2%(P<0.05).基因芯片結果顯示,極低頻磁場聯閤X線組共有1465條齣現上調,1108條齣現下調.其中比值≥2倍的基因共有336條,上調262條,下調74條.與凋亡相關基因共110條,上調基因71條,下調基因39條.涉及CDC25、CHKl、ATM、p38、PTEN、p53、G1/S、Fas、G2/M、Cell Cycle、Apoptosis、Caspase基因通路中的基因.極低頻磁場加X線組差異錶達結果中有13條同時齣現在極低頻磁場組.42條同時齣現在X線組.結論 極低頻磁場與X線通過不同的基因通路影響細胞凋亡.極低頻磁場與X線對人肝癌細胞株BEL-7402凋亡有協同作用.
목적 연구겁저빈자장연합x선대인간암세포주BEL-7402적작용궤제.방법 인간암세포주BEL-7402경0、2、4、6、8、10 Gy X선조사후,용겁저빈자장처리(100 Hz、0.7 mT、30 min、3 d)후,이용소묘전경작형태관찰,류식세포의、기인심편연구세포조망궤제.결과 소묘전경형태현시,겁저빈자장연합X선조간암세포주BEL-7402발생조망개변.류식세포의관찰결과현시,당X선제량시2、4,6、8、10 Gy,겁저빈자장연합x선조망솔위10.0%、14.5%、4.3%、5.1%、7.1%,X선조조망솔시0.1%、8.10%、0.1%、0.4%、2.2%(P<0.05).기인심편결과현시,겁저빈자장연합X선조공유1465조출현상조,1108조출현하조.기중비치≥2배적기인공유336조,상조262조,하조74조.여조망상관기인공110조,상조기인71조,하조기인39조.섭급CDC25、CHKl、ATM、p38、PTEN、p53、G1/S、Fas、G2/M、Cell Cycle、Apoptosis、Caspase기인통로중적기인.겁저빈자장가X선조차이표체결과중유13조동시출현재겁저빈자장조.42조동시출현재X선조.결론 겁저빈자장여X선통과불동적기인통로영향세포조망.겁저빈자장여X선대인간암세포주BEL-7402조망유협동작용.
Objective To investigate the mechanism of extremely low frequency electromagnetic field (ELF-EMF) and X-ray irradiation on liver carcinoma cell lines BEL-7402. Methods Liver carcinoma cell lines BEL-7402 had been incubated with ELF-EMF (100 Hz, 0.7 mT, 30 min, 3 days) after X-ray irradiation at different doses (0, 2, 4, 6, 8,10 Gy). The cells were observed on morphologic changes with scanning electron microscope. Flow cytometry and gene microarray were used to investigate the mechanism of cell apeptosis. Results ELF-EMF plus X-ray induced apoptosis on BEL-7402 was observed under scanning electron microscope.When X-irradiation was 2, 4, 6, 8 and 10 Gy, the apoptosis ratios of combined group and only X-irradiation group were 10.0%, 14.5%, 4.3%, 5.1%, 7.1% and 0.1%, 8.1%, 0.1%, 0.4%, 2.2% (P < 0.05) on flow cytometry. The result of microarray indicated that 1465 genes were up-regulated and 1108 down*regulated in the ELF-EMF plus X-ray group in comparison with the control group. The change rates of 110 apoptosis related genes were above 2 times, which including 71 up-regulated and 39 down-regulated. Gene microarray showed that ELF-EMF and X-ray irradiation had a mainly effect on different gene of apoptosis paths (CDC25 and CHKI, ATM, p38, PTEN, p53, G1/S, Fas, G2/M, Cell Cycle, Apoptosis and Caspase). The same genes of ELF-EMF plus X-ray group were showed 13 in ELF-EMF group and 42 in X-ray group. Conclusions Apoptosis paths were significantly different between ELF-EMF and X-irradiation. ELF-EMF cooperates with X-irradiation on inducing BEL-7402 cell apoptosis.
