CAJ | 학술논문

目的探索阿魏酸、丹参多酚酸对持续性高正加速度引起大鼠颞肌波形蛋白表达的作用. 方法 24只雄性Wistar大鼠随机分为8组,每组3只;第1组只在实验离心机上固定5 min作为对照组;第2组在+10 Gz条件下处置30 s、每日重复5次,每周4次,连续3周作为实验对照组;其余6组处置方法同实验对照组,且每日离心前30 min分别肌肉注射阿魏酸或丹参多酚酸(给药剂量分别为1、3、10 mg/kg),对照组及实验对照组给予等容积的生理盐水.用免疫组化染色方法观察每组波形蛋白的表达情况. 结果对照组未发现波形蛋白的表达,而实验对照组波形蛋白染色呈现强阳性.不同剂量的阿魏酸、丹参多酚酸对于波形蛋白的表达呈现抑制作用,并且该抑制作用具有剂量依赖性. 结论阿魏酸、丹参多酚酸对于持续性高正加速度引起的大鼠颞肌波形蛋白的表达有抑制作用,提示该类药物可有效地防治由于持续性高正加速度引起的颞颌关节疾病.
목적 탐색아위산、단삼다분산대지속성고정가속도인기대서섭기파형단백표체적작용. 방법 24지웅성Wistar대서수궤분위8조,매조3지;제1조지재실험리심궤상고정5 min작위대조조;제2조재+10 Gz조건하처치30 s、매일중복5차,매주4차,련속3주작위실험대조조;기여6조처치방법동실험대조조,차매일리심전30 min분별기육주사아위산혹단삼다분산(급약제량분별위1、3、10 mg/kg),대조조급실험대조조급여등용적적생리염수.용면역조화염색방법관찰매조파형단백적표체정황. 결과 대조조미발현파형단백적표체,이실험대조조파형단백염색정현강양성.불동제량적아위산、단삼다분산대우파형단백적표체정현억제작용,병차해억제작용구유제량의뢰성. 결론 아위산、단삼다분산대우지속성고정가속도인기적대서섭기파형단백적표체유억제작용,제시해류약물가유효지방치유우지속성고정가속도인기적섭합관절질병.
Objective To observe the changes of vimentin expression of temporal muscles in rats that exposed to high sustained +Gz acceleration after administrating ferulic acid and depside salt from salvia mihiorrhiza. Methods Twenty-four male Wistar rats were randomly and averagely divided into 8 groups.Group 1 was control group and in where rats were only fixed on the resting centrifuge for 5 min without drug administration.Others were taken as test groups in where rats were exposed to +10 Gz environment for 30 s.The exposures for each test group were repeated 5 times a day,4 times a week and were tested for 3 weeks.In test groups,group 2 was taken as test control group where without drug administration while rats in other test groups were administrated either ferulic acid or depside salt from salvia mihiorrhiza 30 min prior to experiment with 3 different doses (1,3,10 mg/kg).Rats in control groups were taken 0.9% sodium chloride in same period with equivalent doses.Vimentin expression of temporal muscles was conducted by immunohistochemistry. Results Group 1 was not observed vimentin expression while group 2 showed significant positive.Taking different doses of ferulic acid and depside salt from salvia mihiorrhiza could inhibit expression of vimentin and such inhibition showed dose-dependent feature. Conclusions Ferulic acid and depside salt from salvia miltiorrhiza can inhibit vimentin expression of temporal muscle of rat that exposed in high sustained +Gz environment.It reveals that ferulic acid and depside salt from salvia miltiorrhiza may effectively prevent or cure +Gz induced temporomandibular joint disorder.