中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2009年
11期
828-831
,共4页
翁永强%邱继刚%张益军%袁祖荣%邱双健%汤钊猷
翁永彊%邱繼剛%張益軍%袁祖榮%邱雙健%湯釗猷
옹영강%구계강%장익군%원조영%구쌍건%탕쇠유
癌%肝细胞%Transgelin%预后
癌%肝細胞%Transgelin%預後
암%간세포%Transgelin%예후
Carcinoma hepatocellular%Transgelin%Prognosis
目的 研究肝癌中Transgelin表达水平与肝癌复发及预后的关系.方法 应用Western-blotting技术检测Transgelin在不同转移潜能肝癌细胞系中的表达,以及在临床肝癌手术标本中肝癌、癌旁组织及正常肝组织中的表达,并用免疫组织化学染色验证上述手术标本中Transgelin的表达水平.以Logrank检验及Kaplan-Meier分析其表达水平与肝癌病人总体生存和无瘤生存的关系.结果 Western-blot法显示Transgelin在肝癌高转移潜能细胞系中的表达明显高于低转移潜能细胞系,其在肝癌组织中的表达也明显高于癌旁及正常肝组织,免疫组化结果 与之相类似.Transgelin强阳性表达的病人术后生存率和无瘤生存率明显低于阴性表达者(P<0.01).结论 Transgelin与肝癌转移能力相关,可作为判断预后的指标.
目的 研究肝癌中Transgelin錶達水平與肝癌複髮及預後的關繫.方法 應用Western-blotting技術檢測Transgelin在不同轉移潛能肝癌細胞繫中的錶達,以及在臨床肝癌手術標本中肝癌、癌徬組織及正常肝組織中的錶達,併用免疫組織化學染色驗證上述手術標本中Transgelin的錶達水平.以Logrank檢驗及Kaplan-Meier分析其錶達水平與肝癌病人總體生存和無瘤生存的關繫.結果 Western-blot法顯示Transgelin在肝癌高轉移潛能細胞繫中的錶達明顯高于低轉移潛能細胞繫,其在肝癌組織中的錶達也明顯高于癌徬及正常肝組織,免疫組化結果 與之相類似.Transgelin彊暘性錶達的病人術後生存率和無瘤生存率明顯低于陰性錶達者(P<0.01).結論 Transgelin與肝癌轉移能力相關,可作為判斷預後的指標.
목적 연구간암중Transgelin표체수평여간암복발급예후적관계.방법 응용Western-blotting기술검측Transgelin재불동전이잠능간암세포계중적표체,이급재림상간암수술표본중간암、암방조직급정상간조직중적표체,병용면역조직화학염색험증상술수술표본중Transgelin적표체수평.이Logrank검험급Kaplan-Meier분석기표체수평여간암병인총체생존화무류생존적관계.결과 Western-blot법현시Transgelin재간암고전이잠능세포계중적표체명현고우저전이잠능세포계,기재간암조직중적표체야명현고우암방급정상간조직,면역조화결과 여지상유사.Transgelin강양성표체적병인술후생존솔화무류생존솔명현저우음성표체자(P<0.01).결론 Transgelin여간암전이능력상관,가작위판단예후적지표.
Objective To investigate the correlation of the level of Transgelin expression in hep-tacellular carcinoma with its recurrence and prognosis. Methods The expression of Transgelin in dif-ferent metastatic potential hepatocellular carcinoma cell lines and cancerous lesions, tissue adjacent to cancer lesions and normal liver tissues collected during the operation were detected by Western-blot-ting. The level of Transgelin expression in these clinical samples was accessed by immunohistochemi-cal staining. The correlation between the level of Transgelin expression and the prognosis was analyzed by means of log-rank test and Kaplan-Meier analysis. Results Western blotting test showed that the expression level of Transgelin was significantly higher in high metastatic potential HCC cell line than in low metastatic potential cell line. Meanwhile, it was higher in tumor tissue than in tissue adjacent to cancer lesions or normal liver tissue. Irnmunohistochemical staining test showed the similar result. Pa-tients with positive expression of Transgelin had worse survival and tumor free survival than those with negative ones (P<0.01). Conclusion The expression of Transgelin in hepatocellular carcinoma is correlated with its metastatic potential and this protein could be the biomarker of the prognosis.
