CAJ | 학술논문

目的探讨喉鳞状细胞癌(简称鳞癌)中DJ-1蛋白表达水平与喉鳞癌临床病理特征的关系.方法将1995年1月至2006年1月收治的71例喉鳞癌患者术后肿瘤组织和9例非喉癌者喉黏膜组织应用免疫组织化学法检测,分析其DJ-1蛋白表达水平与临床病理特征的关系.结果喉鳞癌组织中DJ-1蛋白阳性表达率为85.9%(61/71),与非喉痛的喉黏膜组织55.5%(5/9)比较,差异有统计学意义(精确概率法P<0.05).分别就患者的性别、年龄、原发部位、T分期、临床分期、淋巴转移和肿瘤的病理学分级等情况进行分组比较,各组间喉鳞癌组织中DJ-1蛋白表达水平的差异均无统计学意义;DJ-1蛋白高表达患者其肿瘤复发率(53.3%)明显高于DJ-1蛋白低表达患者的复发率(26.8%;x2=5.164,P<0.05).Kaplan-Meier法和Cox回归分析发现喉鳞癌患者的肿瘤原发部位、性别、年龄、T分期、临床分期和肿瘤病理学分级对术后累积生存率影响均无统计学意义;DJ-1蛋白表达水平和淋巴转移对术后累积生存率影响有统计学意义(X2值分别为6.20和3.97,P值均<0.05).结论 DJ-1蛋白在喉鳞癌组织的表达水平高于喉黏膜组织.DJ-1蛋白表达水平较高的喉癌患者生存率可能低.
목적 탐토후린상세포암(간칭린암)중DJ-1단백표체수평여후린암림상병리특정적관계.방법 장1995년1월지2006년1월수치적71례후린암환자술후종류조직화9례비후암자후점막조직응용면역조직화학법검측,분석기DJ-1단백표체수평여림상병리특정적관계.결과 후린암조직중DJ-1단백양성표체솔위85.9%(61/71),여비후통적후점막조직55.5%(5/9)비교,차이유통계학의의(정학개솔법P<0.05).분별취환자적성별、년령、원발부위、T분기、림상분기、림파전이화종류적병이학분급등정황진행분조비교,각조간후린암조직중DJ-1단백표체수평적차이균무통계학의의;DJ-1단백고표체환자기종류복발솔(53.3%)명현고우DJ-1단백저표체환자적복발솔(26.8%;x2=5.164,P<0.05).Kaplan-Meier법화Cox회귀분석발현후린암환자적종류원발부위、성별、년령、T분기、림상분기화종류병이학분급대술후루적생존솔영향균무통계학의의;DJ-1단백표체수평화림파전이대술후루적생존솔영향유통계학의의(X2치분별위6.20화3.97,P치균<0.05).결론 DJ-1단백재후린암조직적표체수평고우후점막조직.DJ-1단백표체수평교고적후암환자생존솔가능저.
Objective To detect the expression of DJ-1 in laryngeal squamous cell carcinoma (LSCC) and to study the relationship between DJ-1 expression and clinical indexes of LSCC. Methods The expressions of DJ-1 protein in 71 LSCC samples and 9 cases control samples from laryngeal mucosa tissues of non-LSCC patients were detected using streptavidin peroxidase immunohistochemistry stainin and the relationships between DJ-1 protein expression and clinicopathologic characteristics were analyzed. Results ( 1 ) The positive expression rate of DJ-1 protein in LSCC was 85.9% (61/71), which was significantly higher than the rate (55.5% ,5/9) in control laryngeal mucosa tissues (P<0.05). (2) DJ-1 expression was related to tumor recurrence (P <0. 05) ,but not to sex,age,primary cancer position,T stage, clinical stage, lymph node metastasis and tumor differentiation. Tumor recurrence rate (53. 3% ) in the patients with higher expression of DJ-1 protein was higher than the rate ( 26. 8% ) in the patients with lower expression of DJ-1 protein ( X2 = 5. 164, P < 0. 05 ). (3 ) With Kaplan-Meier curves and Cox regression analysis,the cumulative 5-year survival rates were correlated with DJ-1 expression levels in laryngeal cancer tissues or cervical lymph node metastasis (all P < 0. 05 ), but not to sex, age, primary cancer position, T stage, clinical stage and tumor differentiation. Conclusions The expression of DJ-1 protein in LSCC is higher than that in control laryngeal mucous tissues. Overexpression of DJ-1 is associated with poor overall survival in ISCC patients.