中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2012年
2期
103-107
,共5页
潘国庆%张翔凌%任俊宇%陆建波%付红梅
潘國慶%張翔凌%任俊宇%陸建波%付紅梅
반국경%장상릉%임준우%륙건파%부홍매
信号素类%胃肿瘤%基因表达%RNA干扰%肿瘤浸润%肿瘤转移%细胞黏附
信號素類%胃腫瘤%基因錶達%RNA榦擾%腫瘤浸潤%腫瘤轉移%細胞黏附
신호소류%위종류%기인표체%RNA간우%종류침윤%종류전이%세포점부
Semaphorins%Stomach neoplasms%Gene expression%RNA interference%Neoplasm invasiveness%Neoplasm metastasis%Cell adhesion
目的 探讨神经轴突导向分子Semaphorin 5A表达与胃癌患者临床病理特征的关系及其在胃癌侵袭和转移过程中的作用.方法 使用免疫组织化学方法检测Semaphorin 5A在171例不同性别、年龄、组织学类型和TNM分期胃癌患者组织中的表达.使用Western印迹方法检测Semaphorin 5A在具转移能力的胃癌细胞株(SGC7901和MKN-45)和无转移能力的胃癌细胞株(SNU-1和AGS)中的表达情况.运用RNA干扰技术构建Semaphorin 5A-siRNA表达载体并转染胃癌细胞株SGC7901,建立Semaphorin 5A稳定低表达的胃癌细胞株,经细胞粘附实验、划痕实验和Transwell小室侵袭实验检测Semaphorin 5A基因沉默后对胃癌细胞粘附、运动和侵袭的影响.结果 Semaphorin 5A表达水平与胃癌分化程度(x2=6.32,P=0.01)、胃癌淋巴结转移(x2=7.68,P=0.01)和远处转移(x2=13.6,P=0.00)有关,与患者年龄(x2=0.21,P=0.79)、性别(x2=1.79,P=0.15)和胃癌浸润深度无关(x2=1.34,P=0.55).SGC7901和MKN-45细胞株中Semaphorin 5A的表达水平明显高于SNU-1和AGS细胞株(P＜0.01).Semaphorin 5A基因表达沉默可抑制胃癌细胞的粘附、运动和侵袭能力.结论 Semaphorin 5A可能通过提高胃癌细胞粘附、运动和侵袭能力,在胃癌侵袭和转移过程中发挥促进作用.
目的 探討神經軸突導嚮分子Semaphorin 5A錶達與胃癌患者臨床病理特徵的關繫及其在胃癌侵襲和轉移過程中的作用.方法 使用免疫組織化學方法檢測Semaphorin 5A在171例不同性彆、年齡、組織學類型和TNM分期胃癌患者組織中的錶達.使用Western印跡方法檢測Semaphorin 5A在具轉移能力的胃癌細胞株(SGC7901和MKN-45)和無轉移能力的胃癌細胞株(SNU-1和AGS)中的錶達情況.運用RNA榦擾技術構建Semaphorin 5A-siRNA錶達載體併轉染胃癌細胞株SGC7901,建立Semaphorin 5A穩定低錶達的胃癌細胞株,經細胞粘附實驗、劃痕實驗和Transwell小室侵襲實驗檢測Semaphorin 5A基因沉默後對胃癌細胞粘附、運動和侵襲的影響.結果 Semaphorin 5A錶達水平與胃癌分化程度(x2=6.32,P=0.01)、胃癌淋巴結轉移(x2=7.68,P=0.01)和遠處轉移(x2=13.6,P=0.00)有關,與患者年齡(x2=0.21,P=0.79)、性彆(x2=1.79,P=0.15)和胃癌浸潤深度無關(x2=1.34,P=0.55).SGC7901和MKN-45細胞株中Semaphorin 5A的錶達水平明顯高于SNU-1和AGS細胞株(P＜0.01).Semaphorin 5A基因錶達沉默可抑製胃癌細胞的粘附、運動和侵襲能力.結論 Semaphorin 5A可能通過提高胃癌細胞粘附、運動和侵襲能力,在胃癌侵襲和轉移過程中髮揮促進作用.
목적 탐토신경축돌도향분자Semaphorin 5A표체여위암환자림상병리특정적관계급기재위암침습화전이과정중적작용.방법 사용면역조직화학방법검측Semaphorin 5A재171례불동성별、년령、조직학류형화TNM분기위암환자조직중적표체.사용Western인적방법검측Semaphorin 5A재구전이능력적위암세포주(SGC7901화MKN-45)화무전이능력적위암세포주(SNU-1화AGS)중적표체정황.운용RNA간우기술구건Semaphorin 5A-siRNA표체재체병전염위암세포주SGC7901,건립Semaphorin 5A은정저표체적위암세포주,경세포점부실험、화흔실험화Transwell소실침습실험검측Semaphorin 5A기인침묵후대위암세포점부、운동화침습적영향.결과 Semaphorin 5A표체수평여위암분화정도(x2=6.32,P=0.01)、위암림파결전이(x2=7.68,P=0.01)화원처전이(x2=13.6,P=0.00)유관,여환자년령(x2=0.21,P=0.79)、성별(x2=1.79,P=0.15)화위암침윤심도무관(x2=1.34,P=0.55).SGC7901화MKN-45세포주중Semaphorin 5A적표체수평명현고우SNU-1화AGS세포주(P＜0.01).Semaphorin 5A기인표체침묵가억제위암세포적점부、운동화침습능력.결론 Semaphorin 5A가능통과제고위암세포점부、운동화침습능력,재위암침습화전이과정중발휘촉진작용.
Objective To explore the correlation between axon guidance factor Semaphorin 5A and clinicopathological features and its role in the invasion and metastasis of gastric cancer.Methods The expression of Semaphorin 5A in gastric cancer tissues of 171 patients with different gender,age,histological type and TNM stage was detected with immunohistochemistry assay.The expression of Semaphorin 5A was determined by Western blotting assay in gastric cancer cell lines SGC7901 and MKN-45 with metastatic ability and gastric cancer cell lines SNU-1 and AGS without metastatic ability.With RNA interfere technique(RNAi),Semaphorin 5A siRNA expression vector was constructed and transfected into gastric cancer cell line SGC7901.The stable gastric cancer cell line down-expressing Semaphorin 5A was established.The effect of Semaphorin 5A gene silencing on the adhesion,migration and invasion of gastric cancer cell was examined by cell adhesion test,wound healing test and transwell chamber assays.Results The expression level of Semaphorin 5A was correlated with the differentiation degree of gastric cancer(x2 =6.32,P =0.01),lymphnode metastasis(x2 =7.68,P=0.01)and distant metastasis of gastric cancer(x2 =13.67,P =0.00),not correlated with age(x2 =0.21,P=0.79),gender(x2=1.79,P=0.15)and the depth of gastric cancer invasion(x2=1.34,P=0.55).The expression of Semaphorin 5A in cell lines SGC7901 and MKN-45 was significantly higher than that of cell lines SNU-1 and AGS(P＜0.01).Semaphorin 5A gene silencing significantly suppressed the adhesion,migration and invasion abilities of gastric cancer cells.Conclusion Semaphorin 5A may play a catalytic role in the invasion and metastasis of gastric cancer through increasing the adhesion,migration and invasion abilities of gastric cancer cell.
