CAJ | 학술논문

目的研究藁本内酯和丁烯酜内酯与大鼠主动脉平滑肌细胞的亲合活性,及其对主动脉平滑肌细胞增殖的抑制作用.方法采用大鼠主动脉细胞膜色谱模型观察藁本内酯和丁烯酜内酯的保留特性;培养并分离纯化大鼠主动脉平滑肌细胞,采用bFGF诱导平滑肌细胞增殖,以MTT比色法检测藁本内酯和丁烯酜内酯对平滑肌细胞增殖的抑制作用.结果藁本内酯和丁烯酜内酯与大鼠主动脉平滑肌细胞膜有亲和性,其保留行为与钙离子受体拮抗剂维拉帕米相似;藁本内酯和丁烯酜内酯不会引起正常大鼠主动脉平滑肌细胞增殖,但能明显抑制bFGF诱导的大鼠主动脉平滑肌细胞的增殖.其有效浓度分别为5.5和11.1 μmol·L-1(P＜0.05).结论藁本内酯和丁烯酜内酯与大鼠主动脉平滑肌细胞具有亲和力,能抑制血管平滑肌细胞的异常增殖.
목적연구고본내지화정희부내지여대서주동맥평활기세포적친합활성,급기대주동맥평활기세포증식적억제작용.방법채용대서주동맥세포막색보모형관찰고본내지화정희부내지적보류특성;배양병분리순화대서주동맥평활기세포,채용bFGF유도평활기세포증식,이MTT비색법검측고본내지화정희부내지대평활기세포증식적억제작용.결과고본내지화정희부내지여대서주동맥평활기세포막유친화성,기보류행위여개리자수체길항제유랍파미상사;고본내지화정희부내지불회인기정상대서주동맥평활기세포증식,단능명현억제bFGF유도적대서주동맥평활기세포적증식.기유효농도분별위5.5화11.1 μmol·L-1(P＜0.05).결론고본내지화정희부내지여대서주동맥평활기세포구유친화력,능억제혈관평활기세포적이상증식.
Aim To investigate the bio-affinities of ligustilide and butylidenephthalide to rat aortic smooth muscle cells and the inhibitory effects of them on bFGF-stimulated proliferation of rat vascular smooth muscle cell (VSMC). Methods VSMCs were cultured from rat aorta pectoralis and identified by an immunohistochemical method. The bio-affinities between solute (ligustilide or butylidenephthalide) and cell membrane were measured by rat aortic cell membrane chromatography (CMC). The inhibitory effects of ligustilide and butylidenephthalide on bFGF-stimulated VSMC proliferation were evaluated by MTT colorimetric method. Results Both ligustilide and butylidenephthalide had selective affinities to rat aortic smooth muscle cell as the same as verapamil, one of the calcium ion antagonists. They could potently separately (P ＜ 0.05 ), but had no effects on the normal VSMC growth. Conclusion Both ligustilide and butylidenephthalide can inhibit the abnormal proliferation of VSMC induced by bFGF.