CAJ | 학술논문

背景:大脑缺血区白细胞与内皮细胞的活化及表面粘附分子的表达可使白细胞聚集浸润,发挥细胞毒作用进而使脑损伤加重.中药川芎嗪具有抗血栓形成,改善微循环的作用.目的:观察川芎嗪对脑缺血-再灌注损伤后局灶区白细胞与内皮细胞粘附性变化的影响.设计:随机对照动物实验.单位:第三军医大学大坪医院脑血管病二科,玉溪市第二人民医院精神科.材料:实验于第三军医大学大坪医院脑血管病二科实验室完成.雄性Wistar大鼠32只,体质量250～300 g,随机分为3组,8只为正常对照组,12只为脑缺血-再灌注组,12只为脑缺血-再灌注加用川芎嗪组.方法:川芎嗪组静脉注射磷酸川芎嗪注射液(15g/kg).正常对照组及脑缺血组予静脉注射等量生理盐水.通过免疫荧光标记技术和显微超高速录像系统观察脑缺血-再灌注及应用川芎嗪后局灶区白细胞粘附性的变化.主要观察指标:①脑缺血-再灌注损伤局灶区微动脉白细胞附壁密度指数及白细胞与内皮细胞之间黏附力.②应用川芎嗪后局灶区白细胞粘附性的变化.结果:实验动物全部进入结果分析.①与对照组比较,脑缺血-再灌注损伤组局灶区微动脉白细胞附壁密度指数增大,白细胞与内皮细胞间断裂应力明显下降,粘附力显著增强,差异均有显著性(P＜0.01).②与脑缺血-再灌注损伤组比较,川芎嗪治疗组白细胞附壁密度指数及粘附力显著下降,至24 h后降至较低水平,断裂应力增高,至24 h与正常对照组差异无显著性,并维持在较高水平.结论:川芎嗪可显著减轻脑缺血-再灌注损伤后内皮细胞与白细胞的粘附.
배경:대뇌결혈구백세포여내피세포적활화급표면점부분자적표체가사백세포취집침윤,발휘세포독작용진이사뇌손상가중.중약천궁진구유항혈전형성,개선미순배적작용.목적:관찰천궁진대뇌결혈-재관주손상후국조구백세포여내피세포점부성변화적영향.설계:수궤대조동물실험.단위:제삼군의대학대평의원뇌혈관병이과,옥계시제이인민의원정신과.재료:실험우제삼군의대학대평의원뇌혈관병이과실험실완성.웅성Wistar대서32지,체질량250～300 g,수궤분위3조,8지위정상대조조,12지위뇌결혈-재관주조,12지위뇌결혈-재관주가용천궁진조.방법:천궁진조정맥주사린산천궁진주사액(15g/kg).정상대조조급뇌결혈조여정맥주사등량생리염수.통과면역형광표기기술화현미초고속록상계통관찰뇌결혈-재관주급응용천궁진후국조구백세포점부성적변화.주요관찰지표:①뇌결혈-재관주손상국조구미동맥백세포부벽밀도지수급백세포여내피세포지간점부력.②응용천궁진후국조구백세포점부성적변화.결과:실험동물전부진입결과분석.①여대조조비교,뇌결혈-재관주손상조국조구미동맥백세포부벽밀도지수증대,백세포여내피세포간단렬응력명현하강,점부력현저증강,차이균유현저성(P＜0.01).②여뇌결혈-재관주손상조비교,천궁진치료조백세포부벽밀도지수급점부력현저하강,지24 h후강지교저수평,단렬응력증고,지24 h여정상대조조차이무현저성,병유지재교고수평.결론:천궁진가현저감경뇌결혈-재관주손상후내피세포여백세포적점부.
BACKGROUND: Activation of leucocyte and endothelial cell in ischemic area of brain and the expression of adhesive molecule on the surface can cause aggregation and infiltration of leucocyte which deteriorated cerebral injury due to cytotoxicity. Ligustrazine can inhibit thrombosis and improve microcirculation.OBJECTIVE: To observe the effect of ligustrazine on adhesion of leucocyte and endothelial cell in focal area after cerebral ischemia-reperfusion injury.DESIGN: Randomized controlled animal study.SETTING: Second Division of Brain, Department of Neurology, Daping Hospital of the Third Military Medical University of Chinese PLA; Department of Psychiatry, the Second People's Hospital of Yuxi, Yunnan Province.MATERIALS: The experiment was carried out at the Second Cerebral Laboratory of Neurological Department of Daping Hospital Affiliated to the Third Military Medical University of Chinese PLA. A total of 32 male Wistar rats weighing 250-300 g were randomly divided into normal control group (n=8), cerebral ischemia-reperfusion group (n=12) and cerebral ischemia-reperfusion + ligustrazine group (n=12).METHODS: Rats in ligustrazine group were venously injected with 15 g/kg ligustrazine phosphoric acid, and rats in normal control group and cerebral ischemia-reperfusion group were venously injected with the same volume of saline. Cerebral ischemia-reperfusion injury and adhesive changes of leucocyte in focal area after administration of ligustrazine were observed with immunofluorescent labeling technique and micro-ultra-speed imaging system.MAIN OUTCOME MEASURES: ① Adhesive density index of leucocyte of arteriole in focal area after cerebral ischemia-reperfusion injury; ② Adhesive changes of leucocyte in focal area after administration of ligustrazine.RESULTS: All experimental animals were involved in the finalanalysis.① As compared with those in control group, adhesive density index of leucocyte of arteriole in focal area in cerebral ischemia-reperfusion group was increased, broken stress between leucocyte and endothelial cell was decreased remarkably, adhesion was increased obviously, and there was significant difference (P ＜ 0.01). ② As compared with those in cerebral ischemia-reperfusion group, adhesive density index and adhesion of leucocyte in ligustrazine group were decreased and reached the lowest values after 24 hours. Broken stress was increased and maintained at a higher level. There was no significant difference from that in normal control group till 24 hours.CONCLUSION: Ligustrazine can relieve adhesion between endothelial cell and leucocyte after cerebral ischemia-reperfusion injury.