CAJ | 학술논문

采用分子生物学检测和不同浓度的NaCl对转BADH基因苜蓿T1代2个株系进行遗传稳定性和抗盐性研究.结果表明,2个株系的T1代PCR阳性植株和阴性植株分离比例都符合孟德尔遗传规律;在0.8%NaCl胁迫下,转基因植株Northern杂交表达量明显增加,BADH酶活性和甜菜碱含量分别比对照植株增加2～4和4～5倍;不同浓度NaCl胁迫下转基因株系脯氨酸和可溶性糖含量、抗氧化酶活性都显著高于对照植株,而电导率和丙二醛含量显著低于对照植株.表明转入的BADH基因可以稳定遗传,转基因苜蓿植株耐盐性明显高于对照.
채용분자생물학검측화불동농도적NaCl대전BADH기인목숙T1대2개주계진행유전은정성화항염성연구.결과표명,2개주계적T1대PCR양성식주화음성식주분리비례도부합맹덕이유전규률;재0.8%NaCl협박하,전기인식주Northern잡교표체량명현증가,BADH매활성화첨채감함량분별비대조식주증가2～4화4～5배;불동농도NaCl협박하전기인주계포안산화가용성당함량、항양화매활성도현저고우대조식주,이전도솔화병이철함량현저저우대조식주.표명전입적BADH기인가이은정유전,전기인목숙식주내염성명현고우대조.
The physiological response of transgenic alfalfa to different salt stresses and its PCR detection were investigated. The ratio of negative to positive numbers of PCR detection confirmed the first Mendelian law. The transgenenic T1 generations showed enhanced BADH activity and betaine content, compared to the non-transgenenic plants at 0.8% NaCl stress. Proline and soluble sugar contents and the antioxidase activity of transgenic T1 generations were higher than those of non-transgenic plants at different salt stresses, while electrolyte leakage and malonaldehyde contents were lower. This suggests that the BADH gene was expressed in the transgenic T1 generation, resulting in improved salt tolerance in the transgenic plants.