CAJ | 학술논문

目的了解浙江猪链球菌2型(SS2)分离株的分子基础及与国内外其他分离株的基因差异程度,确定Cps2J基因变异位点和频率,为该地区的猪链球菌防治提供科学依据.方法提取菌株DNA,应用聚合酶链反应(PCR)扩增菌株Cps2J基因全片段,克隆入质粒载体,纯化后采用ABI自动测序仪测定序列,并同国内外其他分离株的基因进行比较.结果扩增出9株菌株的Cps2J基因的完整开放阅读框(ORF)都为999bp,编码333个氨基酸,9株菌株Cps2J片段核苷酸高度同源,同源性在99.7%～100%;与国内外其他的SS2核苷酸的同源性为98.8%～99.0%,与猪链球菌1型的同源性只有56.8%～57.0%. 结论 9株浙江省猪链球菌2型分离株Cps2J基因序列非常保守,这些不同来源的菌株可能有相同的起源.
목적 료해절강저련구균2형(SS2)분리주적분자기출급여국내외기타분리주적기인차이정도,학정Cps2J기인변이위점화빈솔,위해지구적저련구균방치제공과학의거.방법 제취균주DNA,응용취합매련반응(PCR)확증균주Cps2J기인전편단,극륭입질립재체,순화후채용ABI자동측서의측정서렬,병동국내외기타분리주적기인진행비교.결과 확증출9주균주적Cps2J기인적완정개방열독광(ORF)도위999bp,편마333개안기산,9주균주Cps2J편단핵감산고도동원,동원성재99.7%～100%;여국내외기타적SS2핵감산적동원성위98.8%～99.0%,여저련구균1형적동원성지유56.8%～57.0%. 결론 9주절강성저련구균2형분리주Cps2J기인서렬비상보수,저사불동래원적균주가능유상동적기원.
To explore the molecular characteristics of Streptococcus suis serotype 2(ss2) isolated in Zhejiang province by deciding the variation loci and its variation frequency of Cps2J gene.The total DNA of 9 strains of ss2 isolated in Zhejiang province were extracted and amplifed by PCR. Then,the Cps2J fragments were cloned into plasmid carrier and completely sequenced after purification.Finally,the sequence results of all 9 ss2 isolates were compared with those obtained by other studies around the world.It was found that the open reading fragments of Cps2J in 9 SS2 isolates encoding 333 amino acids were 999 bp in length.Comparisons of this region among ss2 isolates revealed a similarity of between 98.8% and 99.9%, while the homology to ss1 strains varied between 56.8% and 57.0%.Our study shows the sequences of complete Cps2J segment are fairly stable and all these 9 ss2 strains of different sources possibly have the same evolutionary origin.