CAJ | 학술논문

[目的]从Y染色体DNA序列中筛选新的Y-STR基因座,调查其在汉族群体中的遗传多态性.[方法]在人类Y染色体DNA序列中查找Y-STR基因座,确定它们在Y染色体上的定位并测序,与已报道的Y-STR基因座比较.检测汉族(广州地区)无关男性群体中这些Y-STR基因座单体型.[结果]鉴别出5个Y-STR基因座(DYS709,DYS720,DYS721,DYS722和DYS723),其中3个STR基因座是新发现的,另2个基因座与在线报告的重叠.所有5个STR基因座都是男性特有的.它们的扩增产物长度介于185 bp～278 bp.在108例汉族无关男性个体中发现DYS709有6个,DYS720有11个,DYS721有4个,DYS722有6个,DYS723有6个等位基因.在本组群体中发现95种单体型,其中84种只出现1次.单体型多样性达0.997 2±0.001 2.[结论]这组5个Y-STR基因座可望应用于汉族群体的有关研究中.
[목적]종Y염색체DNA서렬중사선신적Y-STR기인좌,조사기재한족군체중적유전다태성.[방법]재인류Y염색체DNA서렬중사조Y-STR기인좌,학정타문재Y염색체상적정위병측서,여이보도적Y-STR기인좌비교.검측한족(엄주지구)무관남성군체중저사Y-STR기인좌단체형.[결과]감별출5개Y-STR기인좌(DYS709,DYS720,DYS721,DYS722화DYS723),기중3개STR기인좌시신발현적,령2개기인좌여재선보고적중첩.소유5개STR기인좌도시남성특유적.타문적확증산물장도개우185 bp～278 bp.재108례한족무관남성개체중발현DYS709유6개,DYS720유11개,DYS721유4개,DYS722유6개,DYS723유6개등위기인.재본조군체중발현95충단체형,기중84충지출현1차.단체형다양성체0.997 2±0.001 2.[결론]저조5개Y-STR기인좌가망응용우한족군체적유관연구중.
[Objective] To screen and identify the new Y-STR loci from the Y chromosome and examine the polymorphism of these Y-STR loci. [Method] To seek and locate the position of 5 Y-STR loci, including DYS709, DYS720, DYS721, DYS722, and DYS723, and perform sequencing of these 5 Y-STR loci. Then to investigate the polymorphism in unrelated Chinese Han males. [Results] Five Y-STR loci were identified from Y chromosome sequence. By scrutinizing the physical position on Y chromosome of previously reported Y-STRs, we found that three loci were novel and two loci overlapped with two loci published only online. All loci could be male-specifically amplified with a product size ranging from 185 bp to 278 bp. After 108 males of the Chinese Han Population (Guangzhou) were examined, we found 5 DYS709, 11 DYS720 alleles, 4 DYS721 alleles, 6 DYS722 alleles, and 6 DYS723 alleles. A total of 95 haplotypes were identified, 84 of which were unique, and with a haplotype diversity of 0.997 2±0.001 2(HD±SE). [Conclusion] This set of Y-STRs can be used as Y chromosome genetic makers in related fields.