CAJ | 학술논문

通过离子化萃取分离的手段将没食子酸与儿茶素进行分离,并采用可见分光光度法对茶叶中没食子酸的含量进行准确测定.此外,还通过模拟实验确定离子化试剂的最佳用量以及溶媒体系的pH值,验证离子化萃取分离的可靠性.与其他用于没食子酸含量测定的方法相比,该方法具有操作简单、成本低、仪器要求不高等优点.将该方法用于检测茶样中没食了酸含量为(1.326±0.018)%,RSD为1.36%,测定值与已见报道相符.
통과리자화췌취분리적수단장몰식자산여인다소진행분리,병채용가견분광광도법대다협중몰식자산적함량진행준학측정.차외,환통과모의실험학정리자화시제적최가용량이급용매체계적pH치,험증리자화췌취분리적가고성.여기타용우몰식자산함량측정적방법상비,해방법구유조작간단、성본저、의기요구불고등우점.장해방법용우검측다양중몰식료산함량위(1.326±0.018)%,RSD위1.36%,측정치여이견보도상부.
In this study,gallic acid and catechin were separated by ionization extraction for the visible spectrophotometric determination of gallic acid content in tea.Simulated experiments were conducted to determine the optimal amount of ionization agent and environmental pH value,and the reliability of ionization extraction was validated.The developed method has some advantages over other methods,like simplicity,low cost and less instrumental requirements.In five replicate determinations,a commeroal sample of Ya' an Tibetan tea was determined by the method to have a gallic acid content of(1.326±0.018)%,with 1.36% relative standard derivation.This result is very close to previously reported values.