目的 探讨氟和铝对大鼠血常规和血生化指标的影响.方法 健康Wistar大鼠56只,体质量130～200 g,按体质量随机分为对照组、低氟组、中氟组、高氟组、低氟+铝组、中氟+铝组、高氟+铝组7组,每组8只,灌胃给予不同剂量的氟化钠和三氯化铝,剂量分别为(0+0)、(100+0)、(200+0)、(300+0)、(100+10)、(200+10)、(300+10)mg/L,时间为90 d.实验期满后,检测血常规,项目包括红细胞数(RBC)、红细胞平均容积(MCV)、白细胞数(WBC)、血红蛋白(HGB)、红细胞比积(HCT)、平均红细胞血红蛋白量(MCH)、平均红细胞血红蛋白浓度(MCHC),血小板数(PIJT)、淋巴细胞数(LYM)、红细胞体积分布宽度(RDW);检测肝、肾功能血清生化指标,包括天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、尿素(Urea)、彤L酐(Cr);称取肝、肾质量,计算脏器系数.结果 大鼠RBC、HCT、MCV各组间比较,差异有统计学意义(F值分别为3.202、3.316、2.915,P均<0.05).RBC、HCT,低氟组[(7.59±2.40)×10~(12)个/L、0.51±0.11]、中氟组[(8.60±1.16)×10~(12)个/L、0.55±0.05]、高氟组[(9.23±0.60)×10~(12)个/L、0.54±0.03]、低氟+铝组[(9.25±0.79)×10~(12)个/L、0.53±0.04]、中氟+铝组[(7.98±2.14)×10~(12)个/L、0.49±0.08]、高氟+铝组[(7.61±3.17)×10~(12)个/L、0.49±0.16]均明显高于对照组[(4.46±3.10)×10~(12)个/L、0.31±0.16,P<0.05或<0.01];MCV,中氟组[(64.06±6.51)fl]、高氟组[(58.67±1.13)fl]、低氟+铝组[(57.78±1.57)fl]、中氟+铝组[(63.04±10.64)fl]均明显高于对照组[(78.54±15.57)fl,P<0.05或<0.01].大鼠血清AST、Urea各组间比较,差异有统计学意义(F值分别为2.847、5.549,P<0.05或<0.01);血清AST水平,低氟组[(399.00±54.99)U/L]、中氟组[(465.60±76.99)U/L]、高氟组[(465.80±75.41)U/L]、低氟+铝组[(346.00±69.26)U/L]、中氟+铝组[(437.40±68.31)U/L]、高氟+铝组[(403.00±30.61)U/L]明显高于对照组[(336.67±94.34)U/L,P<0.05],高氟组明显高于高氟+铝组(P<0.05);血清Urea水平,中氟组[(7.70±0.52)mmol/L]、高氟组[(8.44±1.30)mmol/L]、低氟+铝组[(7.83±0.62)mmol/L]、中氟+铝组[(7.73±0.47)mmol/L]、高氟+铝组[(7.70±0.21)mmol/L]明显高于对照组[(6.55±0.50)mmol/L,P<0.05或<0.01],低氟组明显低于低氟+铝组(P<0.01),高氟组明显高于高氟+铝组(P<0.05).低氟组肝脏脏器系数(2.94±0.36)高于低氟+铝组(2.60±0.15,P<0.05).结论 氟及氟和铝对大鼠血常规和肝、肾功能血清生化指标有一定程度的影响,大鼠红细胞增生而细胞体积变小,质量下降,肝、肾功能受损.铝对不同剂量氟所起的作用不同.
目的 探討氟和鋁對大鼠血常規和血生化指標的影響.方法 健康Wistar大鼠56隻,體質量130～200 g,按體質量隨機分為對照組、低氟組、中氟組、高氟組、低氟+鋁組、中氟+鋁組、高氟+鋁組7組,每組8隻,灌胃給予不同劑量的氟化鈉和三氯化鋁,劑量分彆為(0+0)、(100+0)、(200+0)、(300+0)、(100+10)、(200+10)、(300+10)mg/L,時間為90 d.實驗期滿後,檢測血常規,項目包括紅細胞數(RBC)、紅細胞平均容積(MCV)、白細胞數(WBC)、血紅蛋白(HGB)、紅細胞比積(HCT)、平均紅細胞血紅蛋白量(MCH)、平均紅細胞血紅蛋白濃度(MCHC),血小闆數(PIJT)、淋巴細胞數(LYM)、紅細胞體積分佈寬度(RDW);檢測肝、腎功能血清生化指標,包括天門鼕氨痠氨基轉移酶(AST)、丙氨痠氨基轉移酶(ALT)、堿性燐痠酶(ALP)、尿素(Urea)、彤L酐(Cr);稱取肝、腎質量,計算髒器繫數.結果 大鼠RBC、HCT、MCV各組間比較,差異有統計學意義(F值分彆為3.202、3.316、2.915,P均<0.05).RBC、HCT,低氟組[(7.59±2.40)×10~(12)箇/L、0.51±0.11]、中氟組[(8.60±1.16)×10~(12)箇/L、0.55±0.05]、高氟組[(9.23±0.60)×10~(12)箇/L、0.54±0.03]、低氟+鋁組[(9.25±0.79)×10~(12)箇/L、0.53±0.04]、中氟+鋁組[(7.98±2.14)×10~(12)箇/L、0.49±0.08]、高氟+鋁組[(7.61±3.17)×10~(12)箇/L、0.49±0.16]均明顯高于對照組[(4.46±3.10)×10~(12)箇/L、0.31±0.16,P<0.05或<0.01];MCV,中氟組[(64.06±6.51)fl]、高氟組[(58.67±1.13)fl]、低氟+鋁組[(57.78±1.57)fl]、中氟+鋁組[(63.04±10.64)fl]均明顯高于對照組[(78.54±15.57)fl,P<0.05或<0.01].大鼠血清AST、Urea各組間比較,差異有統計學意義(F值分彆為2.847、5.549,P<0.05或<0.01);血清AST水平,低氟組[(399.00±54.99)U/L]、中氟組[(465.60±76.99)U/L]、高氟組[(465.80±75.41)U/L]、低氟+鋁組[(346.00±69.26)U/L]、中氟+鋁組[(437.40±68.31)U/L]、高氟+鋁組[(403.00±30.61)U/L]明顯高于對照組[(336.67±94.34)U/L,P<0.05],高氟組明顯高于高氟+鋁組(P<0.05);血清Urea水平,中氟組[(7.70±0.52)mmol/L]、高氟組[(8.44±1.30)mmol/L]、低氟+鋁組[(7.83±0.62)mmol/L]、中氟+鋁組[(7.73±0.47)mmol/L]、高氟+鋁組[(7.70±0.21)mmol/L]明顯高于對照組[(6.55±0.50)mmol/L,P<0.05或<0.01],低氟組明顯低于低氟+鋁組(P<0.01),高氟組明顯高于高氟+鋁組(P<0.05).低氟組肝髒髒器繫數(2.94±0.36)高于低氟+鋁組(2.60±0.15,P<0.05).結論 氟及氟和鋁對大鼠血常規和肝、腎功能血清生化指標有一定程度的影響,大鼠紅細胞增生而細胞體積變小,質量下降,肝、腎功能受損.鋁對不同劑量氟所起的作用不同.
목적 탐토불화려대대서혈상규화혈생화지표적영향.방법 건강Wistar대서56지,체질량130～200 g,안체질량수궤분위대조조、저불조、중불조、고불조、저불+려조、중불+려조、고불+려조7조,매조8지,관위급여불동제량적불화납화삼록화려,제량분별위(0+0)、(100+0)、(200+0)、(300+0)、(100+10)、(200+10)、(300+10)mg/L,시간위90 d.실험기만후,검측혈상규,항목포괄홍세포수(RBC)、홍세포평균용적(MCV)、백세포수(WBC)、혈홍단백(HGB)、홍세포비적(HCT)、평균홍세포혈홍단백량(MCH)、평균홍세포혈홍단백농도(MCHC),혈소판수(PIJT)、림파세포수(LYM)、홍세포체적분포관도(RDW);검측간、신공능혈청생화지표,포괄천문동안산안기전이매(AST)、병안산안기전이매(ALT)、감성린산매(ALP)、뇨소(Urea)、동L항(Cr);칭취간、신질량,계산장기계수.결과 대서RBC、HCT、MCV각조간비교,차이유통계학의의(F치분별위3.202、3.316、2.915,P균<0.05).RBC、HCT,저불조[(7.59±2.40)×10~(12)개/L、0.51±0.11]、중불조[(8.60±1.16)×10~(12)개/L、0.55±0.05]、고불조[(9.23±0.60)×10~(12)개/L、0.54±0.03]、저불+려조[(9.25±0.79)×10~(12)개/L、0.53±0.04]、중불+려조[(7.98±2.14)×10~(12)개/L、0.49±0.08]、고불+려조[(7.61±3.17)×10~(12)개/L、0.49±0.16]균명현고우대조조[(4.46±3.10)×10~(12)개/L、0.31±0.16,P<0.05혹<0.01];MCV,중불조[(64.06±6.51)fl]、고불조[(58.67±1.13)fl]、저불+려조[(57.78±1.57)fl]、중불+려조[(63.04±10.64)fl]균명현고우대조조[(78.54±15.57)fl,P<0.05혹<0.01].대서혈청AST、Urea각조간비교,차이유통계학의의(F치분별위2.847、5.549,P<0.05혹<0.01);혈청AST수평,저불조[(399.00±54.99)U/L]、중불조[(465.60±76.99)U/L]、고불조[(465.80±75.41)U/L]、저불+려조[(346.00±69.26)U/L]、중불+려조[(437.40±68.31)U/L]、고불+려조[(403.00±30.61)U/L]명현고우대조조[(336.67±94.34)U/L,P<0.05],고불조명현고우고불+려조(P<0.05);혈청Urea수평,중불조[(7.70±0.52)mmol/L]、고불조[(8.44±1.30)mmol/L]、저불+려조[(7.83±0.62)mmol/L]、중불+려조[(7.73±0.47)mmol/L]、고불+려조[(7.70±0.21)mmol/L]명현고우대조조[(6.55±0.50)mmol/L,P<0.05혹<0.01],저불조명현저우저불+려조(P<0.01),고불조명현고우고불+려조(P<0.05).저불조간장장기계수(2.94±0.36)고우저불+려조(2.60±0.15,P<0.05).결론 불급불화려대대서혈상규화간、신공능혈청생화지표유일정정도적영향,대서홍세포증생이세포체적변소,질량하강,간、신공능수손.려대불동제량불소기적작용불동.
Objective To study the effects of fluorine and aluminum on index of hematologic tests of rats. Methods According to body mass,56 Wistar rats of 130-200 g were randomly divided into control,low-fluorine (F),middle-F,high-F,low-F + aluminum(Al),middle-F + Al,high-F + Al group,8 rats in each group were given a series of doses of fluoride and aluminum,which were (0 + 0),(100 + 0),(200 + 0),(300 + 0),(100 + 10),(200 + 10),(300 + 10)mg/L After 90-day intragastrie administration,blood samples were collected on eyes of rats to undergo blood routine test,including red blood cell (RBC),lymphocyte (LYM),platelet (PLT),hemoglobin (HGB),white blood cell (WBC),hematocrit (HCT),mean corpuscular hemoglobin (MCH),mean corpuscular-hemoglobin concentration(MCHC),mean corpuscular volume(MCV),and at the same time some blood biochemistry indicators related to functio ns of liver and kidney were determined such as aspartic acid aminotransferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(ALP),Crea(Cr) and Urea. Organ coefficient of liver and kidney were calculated. Results The difference of RBC,HCT,MCV among all groups of rats was statistically significant(F = 3.202,3.316,2.915,P < 0.05). The RBC,HCT of the low-F group[(7.59± 2.40)×10~(12)/L,0.51±0.11],the middle-F group[(8.60±1.16)×10~(12)/L,0.55±0.05],the high-F group[(9.23± 0.60)×10~(12)/L,0.54±0.03],the low-F + Al group[(9.25±0.79)×10~(12)/L,0.53±0.04],the middle-F + Al group[(7.98±2.14)×10~(12)/L,0.49±0.08]and the high-F + Al group[(7.61±3.17)×10~(12)/L,0.49±0.16]were significantly higher than that in the control group[(4.46±3.10)×10~(12)/L,0.31±0.16,P< 0.05 or < 0.01)]. The MCV of the middle-F group[(64.06±6.51)fl],high-F group[(58.67±1.13)fl],low-F + Al group[(57.78± 1.57)fl]and the middle-F + Al group[(63.04±10.64)fl]were significantly higher than the control group[(78.54± 15.57)fl,P < 0.05 or < 0.01]. The difference of AST and Urea among all the groups of mrs serum was statistically significant(F= 2.847,5.549,P < 0.05 or < 0.01). The serum AST of low-F group[(399.00±54.99)U/L],the middle-Fgroup[(465.60±76.99)U/L],the high-F group[(465.80±75.41)U/L],the low-F + Al group[(346.00±69.26) U/L],the middle-F + Al group[(437.40±68.31)U/L]and the high-F + Al group[(403.00±30.61)U/L]were all significantly higher than that in the control group[(336.67±94.34)U/L,P < 0.05],and the high-F group significantly higher than the high-F + Al group(P < 0.05). The serum Urea of the middle-F group[(7.70±0.52)mmol/L],the high-F group[(8.44±1.30)mmol/L],the low-F + Al group[(7.83±0.62)mmol/L],the middle-F + Al group [(7.73±0.47)mmol/L],and the high fluoride + aluminum group[(7.70±0.21)mmol/L]were all significantly higher than that in the control group[(6.55±0.50)mmol/L,P< 0.05 or < 0.01],and the low-F group was significantly lower than the low-F + Al group(P < 0.01),however the high-F group was significantly higher than that in the high-F + Al group(P< 0.05). The liver organ coefficient of the low-F group(2.94±0.36) was higher than the low-F + Al group (2.60±0.15,P < 0.05). Conclusions Fluorine and combination of aluminum and fluorine have toxicity on rats to a certain extent,including the proliferation of crythrocytes of rat,while the cell size gets smaller and the cell quality is deteriorated,meanwhile functions of liver and kidney are impaired. Aluminum shows different joint action in different concentrations of fluorine.
