国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2009年
5期
418-421
,共4页
神经病理性疼痛%细胞周期素依赖性激酶-5%脊髓背角
神經病理性疼痛%細胞週期素依賴性激酶-5%脊髓揹角
신경병이성동통%세포주기소의뢰성격매-5%척수배각
Neuropathic pain%Cyclin-dependent kinase 5%Spinal dorsal horn
目的 探讨鞘内注射细胞周期素依赖性激酶-5(Cdk5)抑制剂Roscovitine对慢性背根节压迫(CCD)大鼠痛行为学的影响.方法 雄性SD大鼠24只随机分为4组,每组6只:假手术组+50%二甲基亚砜(S组);CCD+50%二甲基哑砜(C组):CCD+Roscovitine 50μg(R组);CCD0组(C0组)C0组不注入任何药物,只作为对照.各组大鼠在CCD或假手术后第7天分别鞘内注射容积为10μl的50%DMSO或Roscovitine 50μg,分别观察给药后的1、4、8 h大鼠机械缩爪阂值(paw withdrawal mechani-cal threshold,PWMT)和热缩爪潜伏期(paw withdrawal thermal latency,PWTL).结果 与基础值相比,除S组外,各组大鼠从术后开始PWMT和PWTL均明显降低(P＜0.05),R组给药后与C组相比PWMT在各时间点无明显改变,PWTL相比明显增加,且保持在较高水平(P＜0.05).结论 脊髓背角Cdk5参与神经病理性疼痛的信号转导过程.
目的 探討鞘內註射細胞週期素依賴性激酶-5(Cdk5)抑製劑Roscovitine對慢性揹根節壓迫(CCD)大鼠痛行為學的影響.方法 雄性SD大鼠24隻隨機分為4組,每組6隻:假手術組+50%二甲基亞砜(S組);CCD+50%二甲基啞砜(C組):CCD+Roscovitine 50μg(R組);CCD0組(C0組)C0組不註入任何藥物,隻作為對照.各組大鼠在CCD或假手術後第7天分彆鞘內註射容積為10μl的50%DMSO或Roscovitine 50μg,分彆觀察給藥後的1、4、8 h大鼠機械縮爪閡值(paw withdrawal mechani-cal threshold,PWMT)和熱縮爪潛伏期(paw withdrawal thermal latency,PWTL).結果 與基礎值相比,除S組外,各組大鼠從術後開始PWMT和PWTL均明顯降低(P＜0.05),R組給藥後與C組相比PWMT在各時間點無明顯改變,PWTL相比明顯增加,且保持在較高水平(P＜0.05).結論 脊髓揹角Cdk5參與神經病理性疼痛的信號轉導過程.
목적 탐토초내주사세포주기소의뢰성격매-5(Cdk5)억제제Roscovitine대만성배근절압박(CCD)대서통행위학적영향.방법 웅성SD대서24지수궤분위4조,매조6지:가수술조+50%이갑기아풍(S조);CCD+50%이갑기아풍(C조):CCD+Roscovitine 50μg(R조);CCD0조(C0조)C0조불주입임하약물,지작위대조.각조대서재CCD혹가수술후제7천분별초내주사용적위10μl적50%DMSO혹Roscovitine 50μg,분별관찰급약후적1、4、8 h대서궤계축조애치(paw withdrawal mechani-cal threshold,PWMT)화열축조잠복기(paw withdrawal thermal latency,PWTL).결과 여기출치상비,제S조외,각조대서종술후개시PWMT화PWTL균명현강저(P＜0.05),R조급약후여C조상비PWMT재각시간점무명현개변,PWTL상비명현증가,차보지재교고수평(P＜0.05).결론 척수배각Cdk5삼여신경병이성동통적신호전도과정.
Objective To study the effects of intrathecal injeetion of cyclin-dependent kinase 5 inhibitor Roseovitine on the pain behavior in chronic compression of dosal root ganglia(CCD)rats.Methods 24 male SD rats with intrathecal catheter properly inserted,were randomly divided into four groups(n=6):sham operation group(S group),50%DMSO was used as the vehicle control for the intrathecal injection;CCD group treated with 50%DMSO(operated for chronic compression of right dorsal root ganglion,C group);Roscovitine group(Roscovitine 50μg,R group)and CCD0 group was not injected any drugs(C0 group),just as that in control.To evaluate the behaviorsl changes,paw withdrawal mechanical threshold(PWMT)and paw withdrawal thermal latency (PWTL)were measured 0,7 days and 1,4,8 h after injection on the 7th day of CCD.Results Compared with the value-based,with the exception of S group,all of the rats after CCD PWMT and PWTL were significantly lower(P＜0.05).PWMT of R group was no significant change at all times point.Compared to value-based PWTL of R group was a marked increase.and remained at a relatively high level(P＜0.05).Conclusion Cdk5 involves in spinal dorsal horn of neuropathic pain signal transduction process.
