CAJ | 학술논문

目的探讨IL-23/IL-17炎症轴在溃疡性结肠炎(UC)中的变化及意义.方法按照2007年济南标准选择UC组患者20例,对照组16例.利用细胞内细胞因子染色和四色荧光抗体流式细胞术对肠黏膜固有层单个核细胞作表型分析,比较Th1、Th2、Th17比例的改变.Western blot检测肠黏膜IL-17、IL-23表达.数据以中位数和四分位间距即M(Q~R)形式表示,行相关统计.结果 (1)肠黏膜中Th17的比例在UC组中较对照组明显增加(P<0.05),为3.75%比1.25%,且重度活动较轻度活动患者增加明显(P<0.05),为8.30%比1.20%.肠黏膜中Th1的比例在UC组和对照组中分别为13.60%和9.10%,Th2的比例在UC和对照组中分别为1.10%和1.15%,差异均无统计学意义(P>0.05),不同活动度患者间差异亦无统计学意义(P>0.05).(2)UC组患者肠黏膜中IL-17表达较对照组明显升高(P<0.05),为0.20%比0.10%,且IL-17表达与UC患者疾病评分呈正相关(r=0.50,P=0.02).(3)IL-23表达在UC组和对照组分别是0.13%和0.07%,差异有统计学意义(P<0.05).结论 IL-23/IL-17炎症轴在溃疡性结肠炎的发病中具有重要作用,它可能成为UC治疗的有效靶点.
목적 탐토IL-23/IL-17염증축재궤양성결장염(UC)중적변화급의의.방법 안조2007년제남표준선택UC조환자20례,대조조16례.이용세포내세포인자염색화사색형광항체류식세포술대장점막고유층단개핵세포작표형분석,비교Th1、Th2、Th17비례적개변.Western blot검측장점막IL-17、IL-23표체.수거이중위수화사분위간거즉M(Q~R)형식표시,행상관통계.결과 (1)장점막중Th17적비례재UC조중교대조조명현증가(P<0.05),위3.75%비1.25%,차중도활동교경도활동환자증가명현(P<0.05),위8.30%비1.20%.장점막중Th1적비례재UC조화대조조중분별위13.60%화9.10%,Th2적비례재UC화대조조중분별위1.10%화1.15%,차이균무통계학의의(P>0.05),불동활동도환자간차이역무통계학의의(P>0.05).(2)UC조환자장점막중IL-17표체교대조조명현승고(P<0.05),위0.20%비0.10%,차IL-17표체여UC환자질병평분정정상관(r=0.50,P=0.02).(3)IL-23표체재UC조화대조조분별시0.13%화0.07%,차이유통계학의의(P<0.05).결론 IL-23/IL-17염증축재궤양성결장염적발병중구유중요작용,타가능성위UC치료적유효파점.
Objective To explore the role of IL-23/IL-17 inflammation axis in pathogenesis of ul-cerative colitis (UC). Methods A total of 20 UC cases diagnosed according to 2007 Jinan criteria and 16 healthy subjects were recruited. The distribution of Th1, Th2 and Th17 cells in colonic lamina propria was analyzed with intracellular cytokine staining and four-color- fluorescence antibody flow cytometry. Western blot was used to determine the expressions of IL-23 and IL-17 in colonic mucosa. Results The proportion of Th17 cell in colonic mucosa was significantly higher in UC patients (3.75%) than in controls (1.25%) (P<0.05), as well as in severely active patients (8.30%) than in slightly active ones (1.20%)(P< 0.05). No significant differences were found in the proportions of Th1 (13.6% vs. 9.10%) and Th2 (1.10% vs. 1.15%) in colonic mucosa between UC and controls, nor were they found between patients of different severity. IL-17 expression in colonic mucosa increased in UC patients than that in controls (0.20% vs. 0.10%) (P<0.05), and was positively correlated with disease severity (r=0.50, P=0.02). IL-23 expression in colonic mucosa was also significantly higher in UC (0.13%) than that in controls (0.07%) (P<0.05). Conclusion IL-23/IL-17 inflammation axis plays an important role in the pathogenesis of UC, which might be a target for treatment.