肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2010年
1期
35-37
,共3页
赵进明%戎铁华%傅剑华%吴兆红%陈岗东%王戈菲
趙進明%戎鐵華%傅劍華%吳兆紅%陳崗東%王戈菲
조진명%융철화%부검화%오조홍%진강동%왕과비
癌,非小细胞肺%细胞凋亡%反转录聚合酶链反应%XIAP%Survivin
癌,非小細胞肺%細胞凋亡%反轉錄聚閤酶鏈反應%XIAP%Survivin
암,비소세포폐%세포조망%반전록취합매련반응%XIAP%Survivin
Carcinoma,non-small-cell lung%Apoptosis%Reverse transciptase polymeroase chain reacltion%XIAP%Survivin
目的 检测非小细胞肺癌(NSCLC)中X连锁凋亡抑制蛋白(XIAP)mRNA和SurvivinmRNA的表达,探讨其与患者临床病理因素的相关性.方法 收集59例NSCLC患者肿瘤和正常组织标本.采用RT-PCR检测XIAP mRNA和Survivin mRNA在肿瘤和正常组织中的表达.结果 XIAP mRNA在肿瘤组织的表达率高于正常组织,分别为61.0%(36/59)和30.5%(18/59)(P<0.05),其表达与临床病理因素无相关性;Survivin mRNA在肿瘤的表达率高于正常组织,分别为81.4%(48/59)和23.7%(14/59)(P<0.05),其表达与临床病理因素无相关性.结论 XIAP和Survivin可能在NSCLC发生、发展过程中有一定作用,有可能成为NSCLC诊断的标志物和治疗靶点.
目的 檢測非小細胞肺癌(NSCLC)中X連鎖凋亡抑製蛋白(XIAP)mRNA和SurvivinmRNA的錶達,探討其與患者臨床病理因素的相關性.方法 收集59例NSCLC患者腫瘤和正常組織標本.採用RT-PCR檢測XIAP mRNA和Survivin mRNA在腫瘤和正常組織中的錶達.結果 XIAP mRNA在腫瘤組織的錶達率高于正常組織,分彆為61.0%(36/59)和30.5%(18/59)(P<0.05),其錶達與臨床病理因素無相關性;Survivin mRNA在腫瘤的錶達率高于正常組織,分彆為81.4%(48/59)和23.7%(14/59)(P<0.05),其錶達與臨床病理因素無相關性.結論 XIAP和Survivin可能在NSCLC髮生、髮展過程中有一定作用,有可能成為NSCLC診斷的標誌物和治療靶點.
목적 검측비소세포폐암(NSCLC)중X련쇄조망억제단백(XIAP)mRNA화SurvivinmRNA적표체,탐토기여환자림상병리인소적상관성.방법 수집59례NSCLC환자종류화정상조직표본.채용RT-PCR검측XIAP mRNA화Survivin mRNA재종류화정상조직중적표체.결과 XIAP mRNA재종류조직적표체솔고우정상조직,분별위61.0%(36/59)화30.5%(18/59)(P<0.05),기표체여림상병리인소무상관성;Survivin mRNA재종류적표체솔고우정상조직,분별위81.4%(48/59)화23.7%(14/59)(P<0.05),기표체여림상병리인소무상관성.결론 XIAP화Survivin가능재NSCLC발생、발전과정중유일정작용,유가능성위NSCLC진단적표지물화치료파점.
Objective To investigate the clinical significance of the expression of XIAP mRNA and Survivin mRNA in non-small cell lung cancer and their relationship. Methods RT-PCR was used to evaluate the expression of XIAP mRNA and Survivin mRNA in 59 cases of NSCLC and corresponding normal tissues.Results There was a significant difference in XIAP mRNA expression in primary lung and corresponding normal tissues (61.0 % vs 30.5 %, P<0.05), whereas there were no significant correlation between the XIAP mRNA expression and gender, age, smoking history, histological subtype, T stage, lymph node metastatic status and TNM stage. There was a significant difference in Survivin mRNA expression in primary lung and corresponding normal tissues (81.4 % vs 23.7 %, P<0.05), whereas there were no significant correlation between the Survivin mRNA expression and gender, age, smoking history, histological subtype, T stage, lymph node metastatic status and TNM stage. Conclusion The significant difference of XIAP mRNA and Survivin mRNA expression between the tumor and corresponding normal tissues implies they might play important roles in the carcinogenesis and progress of NSCLC and might become marker for the diagnosis and target for treatment of NSCLC.
