中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2009年
6期
462-465
,共4页
光刺激/副作用%色素上皮%眼/细胞学%细胞衰老/生理学%动物实验
光刺激/副作用%色素上皮%眼/細胞學%細胞衰老/生理學%動物實驗
광자격/부작용%색소상피%안/세포학%세포쇠로/생이학%동물실험
Photoc stimulation/adverse effects%Pigment epithelium of eye/cytology%Cell aging/physiology%Animal experimentation
目的 探讨蓝光光照强度、时间与大鼠视网膜色素上皮(RPE)细胞复制衰老的关系.方法 将36只鼠龄12～14周的Wistar大鼠置于悬有波长为(450±10)nm的医用蓝光灯管的自制光照架中.随机分为4组,每组9只大鼠,1组:无光照对照组;2组:自然光照组;3组:500 lx光照组;4组:1000 lx光照组.每组按照照射时间再分为1、2、3个月组3个亚组,分别在1、2、3个月时行10%水合氯醛腹腔注射麻醉后取出眼球,将右眼球行石蜡切片,苏木精伊红(HE)染色;左眼球行冰冻切片,采用衰老相关β半乳糖苷酶(SA-β-Gal)染色方法观察RPE细胞染色阳性情况.采用SPSS 11.5统计软件对结果数据进行统计学分析.结果 不同光照强度组、不同光照时间组大鼠RPE细胞SA-β-Gal染色阳性细胞个数差异有统计学意义(F=510.309,55.016;P=0.000);组间两两比较,除1组与2组之间差异无统计学意义外(P=0.154),其它各组之间差异均有统计学意义(P=0.000).在单一光照强度条件下,除1组大鼠RPE细胞SA-β-Gal染色阳性细胞个数差异无统计学意义外(P=0.897),其余各组差异均有统计学意义(P＜0.01);在单一时间条件下,各组差异均有统计学意义(P=0.000).结论 同一蓝光光照强度下,随着时间的增加大鼠视网膜RPE细胞逐渐出现复制衰老改变;同一时间条件下,随光照强度增加大鼠视网膜RPE细胞也逐渐出现复制衰老改变.
目的 探討藍光光照彊度、時間與大鼠視網膜色素上皮(RPE)細胞複製衰老的關繫.方法 將36隻鼠齡12～14週的Wistar大鼠置于懸有波長為(450±10)nm的醫用藍光燈管的自製光照架中.隨機分為4組,每組9隻大鼠,1組:無光照對照組;2組:自然光照組;3組:500 lx光照組;4組:1000 lx光照組.每組按照照射時間再分為1、2、3箇月組3箇亞組,分彆在1、2、3箇月時行10%水閤氯醛腹腔註射痳醉後取齣眼毬,將右眼毬行石蠟切片,囌木精伊紅(HE)染色;左眼毬行冰凍切片,採用衰老相關β半乳糖苷酶(SA-β-Gal)染色方法觀察RPE細胞染色暘性情況.採用SPSS 11.5統計軟件對結果數據進行統計學分析.結果 不同光照彊度組、不同光照時間組大鼠RPE細胞SA-β-Gal染色暘性細胞箇數差異有統計學意義(F=510.309,55.016;P=0.000);組間兩兩比較,除1組與2組之間差異無統計學意義外(P=0.154),其它各組之間差異均有統計學意義(P=0.000).在單一光照彊度條件下,除1組大鼠RPE細胞SA-β-Gal染色暘性細胞箇數差異無統計學意義外(P=0.897),其餘各組差異均有統計學意義(P＜0.01);在單一時間條件下,各組差異均有統計學意義(P=0.000).結論 同一藍光光照彊度下,隨著時間的增加大鼠視網膜RPE細胞逐漸齣現複製衰老改變;同一時間條件下,隨光照彊度增加大鼠視網膜RPE細胞也逐漸齣現複製衰老改變.
목적 탐토람광광조강도、시간여대서시망막색소상피(RPE)세포복제쇠로적관계.방법 장36지서령12～14주적Wistar대서치우현유파장위(450±10)nm적의용람광등관적자제광조가중.수궤분위4조,매조9지대서,1조:무광조대조조;2조:자연광조조;3조:500 lx광조조;4조:1000 lx광조조.매조안조조사시간재분위1、2、3개월조3개아조,분별재1、2、3개월시행10%수합록철복강주사마취후취출안구,장우안구행석사절편,소목정이홍(HE)염색;좌안구행빙동절편,채용쇠로상관β반유당감매(SA-β-Gal)염색방법관찰RPE세포염색양성정황.채용SPSS 11.5통계연건대결과수거진행통계학분석.결과 불동광조강도조、불동광조시간조대서RPE세포SA-β-Gal염색양성세포개수차이유통계학의의(F=510.309,55.016;P=0.000);조간량량비교,제1조여2조지간차이무통계학의의외(P=0.154),기타각조지간차이균유통계학의의(P=0.000).재단일광조강도조건하,제1조대서RPE세포SA-β-Gal염색양성세포개수차이무통계학의의외(P=0.897),기여각조차이균유통계학의의(P＜0.01);재단일시간조건하,각조차이균유통계학의의(P=0.000).결론 동일람광광조강도하,수착시간적증가대서시망막RPE세포축점출현복제쇠로개변;동일시간조건하,수광조강도증가대서시망막RPE세포야축점출현복제쇠로개변.
Objective To investigate the relationship between exposure intensity and illumination time of blue light and replicative senescence of rat retinal pigment epithelial (RPE) ceils.Methods Thirty-six 12-14 weeks Wistar rats were kept in the cage with a blue-light bulb [(450±10) nm],and were randomly divided into four groups (no light,nature light,500 lx light and 1000 lx light illumination),each has nine rats.The rats in each group were further divided into three subgroups according to illumination time (one month,two months or three months).Eyeballs were collected after intraperitoneal injection of 10% chloral hydrate.The right eye of each rat was embedded in paraffin and sectioned for hematoxylin-eosin (HE)staining,while frozen sections of the left eye were stained for the senescence-associated β-galactosidase (SA-β-Gal).The data were analyzed by SPSS11.5 statistical software.Results The amounts of SA-β-Gal positive RPE cells were significantly different between all groups under the same illumination time 17 (P=0.000),and between all subgroups of different illumination time with same exposure intensity (P＜0.01)except for the control group (no light).Conclusion Blue-light can induce replicative senescence in rat RPE cells in an intensity and time-dependent manner.
