清华大学学报(英文版)
清華大學學報(英文版)
청화대학학보(영문판)
TSINGHUA SCIENCE AND TECHNOLOGY
2003年
4期
466-470
,共5页
丘远征%刘力平%陈国强
丘遠徵%劉力平%陳國彊
구원정%류력평%진국강
polyhydroxyalkanoates (PHA)%poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)%Aeromonas hydrophila%recombinant Escherichia coli
Aeromonas hydrophila (A.hydrophila) 4AK4 produced poly(3-hydroxybutyrate-co-3- hydroxyhexanoate) (PHBHHx) with an almost constant 3-hydroxyhexanoate (3HHx) content of 10%-15% from lauric acid and/or soybean oil.Both A.hydrophila 4AK4 and recombinant Escherichia coli (E.coli) JMU193 (pBH32) produced PHBHHx with controllable 3HHx content when fed lauric acid and another co-substrate.With glucose or gluconate as the co-substrate, the 3HHx content in the copolyester produced by A.hydrophila 4AK4 was reduced slightly from 12% to 9%.However, the 3HHx content in the copolyester produced by E.coli JMU193 (pBH32) was significantly reduced from 9% to 2% with fructose as the co-substrate.These results show that regulation of 3HHx content in PHBHHx can be achieved using genetically engineered E.coli.
