国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2008年
10期
1963-1967
,共5页
柚皮素%人视网膜色素上皮细胞%人脐静脉内皮细胞%老年黄斑变性
柚皮素%人視網膜色素上皮細胞%人臍靜脈內皮細胞%老年黃斑變性
유피소%인시망막색소상피세포%인제정맥내피세포%노년황반변성
naringenin%ARPE-19 cells%HUVEC%AMD
目的:研究柚皮素对人视网膜色素上皮细胞(ARPE-19)和人脐静脉内皮细胞(HUVEC)的抗氧化作用.方法:采用MTT.的方法检测ARPE-19和HUVEC细胞的生存率及增殖率.结果:3,10mg/L柚皮素能显著增加ARPE-19细胞的增殖率达10.8%和11.4%.10mg/L柚皮素能提高ARPE-19细胞在缺氧,0.3mmol/L NaN3及200Fμmol/L H2O2条件下的生存率分别为55.2%,69.2%及50.3%.1mg/L柚皮素能提高ARPE-19细胞在50μmoL/L t-BHP和30mg/LNaIO3条件下的生存率达20.2%和30.4%.30mg/L柚皮素能够促进ARPE-19细胞在50 μmol/L t-BHP条件下的增殖率达32.2%,而1mg/L柚皮素可以提高30,100,300mg/L NaIO3处理的ARPE-19细胞的增殖率达30.3%,10.3%及18.5%.3,10及30mg/L柚皮素抑制HUVEC的增殖率分别为23.9%,70.4%及77.9%.1,3mg/L柚皮素能提高HUVEC细胞在缺氧条件下的生存率达10.7%和13.1%,以及提高在300mg/L NaIO3条件下的生存率达41.2%和37.7%.3mg/L柚皮素能提高HUVEC细胞在200,4001xmol/L H2O2,条件下的生存率达20.1%和21.5%.结论:柚皮素能够促进ARPE-19细胞的增殖率,抑制HU-VEC生长,同时对这两种细胞均有抗氧化作用.因此,柚皮素是治疗老年黄斑变性的很有前景的候选药物.
目的:研究柚皮素對人視網膜色素上皮細胞(ARPE-19)和人臍靜脈內皮細胞(HUVEC)的抗氧化作用.方法:採用MTT.的方法檢測ARPE-19和HUVEC細胞的生存率及增殖率.結果:3,10mg/L柚皮素能顯著增加ARPE-19細胞的增殖率達10.8%和11.4%.10mg/L柚皮素能提高ARPE-19細胞在缺氧,0.3mmol/L NaN3及200Fμmol/L H2O2條件下的生存率分彆為55.2%,69.2%及50.3%.1mg/L柚皮素能提高ARPE-19細胞在50μmoL/L t-BHP和30mg/LNaIO3條件下的生存率達20.2%和30.4%.30mg/L柚皮素能夠促進ARPE-19細胞在50 μmol/L t-BHP條件下的增殖率達32.2%,而1mg/L柚皮素可以提高30,100,300mg/L NaIO3處理的ARPE-19細胞的增殖率達30.3%,10.3%及18.5%.3,10及30mg/L柚皮素抑製HUVEC的增殖率分彆為23.9%,70.4%及77.9%.1,3mg/L柚皮素能提高HUVEC細胞在缺氧條件下的生存率達10.7%和13.1%,以及提高在300mg/L NaIO3條件下的生存率達41.2%和37.7%.3mg/L柚皮素能提高HUVEC細胞在200,4001xmol/L H2O2,條件下的生存率達20.1%和21.5%.結論:柚皮素能夠促進ARPE-19細胞的增殖率,抑製HU-VEC生長,同時對這兩種細胞均有抗氧化作用.因此,柚皮素是治療老年黃斑變性的很有前景的候選藥物.
목적:연구유피소대인시망막색소상피세포(ARPE-19)화인제정맥내피세포(HUVEC)적항양화작용.방법:채용MTT.적방법검측ARPE-19화HUVEC세포적생존솔급증식솔.결과:3,10mg/L유피소능현저증가ARPE-19세포적증식솔체10.8%화11.4%.10mg/L유피소능제고ARPE-19세포재결양,0.3mmol/L NaN3급200Fμmol/L H2O2조건하적생존솔분별위55.2%,69.2%급50.3%.1mg/L유피소능제고ARPE-19세포재50μmoL/L t-BHP화30mg/LNaIO3조건하적생존솔체20.2%화30.4%.30mg/L유피소능구촉진ARPE-19세포재50 μmol/L t-BHP조건하적증식솔체32.2%,이1mg/L유피소가이제고30,100,300mg/L NaIO3처리적ARPE-19세포적증식솔체30.3%,10.3%급18.5%.3,10급30mg/L유피소억제HUVEC적증식솔분별위23.9%,70.4%급77.9%.1,3mg/L유피소능제고HUVEC세포재결양조건하적생존솔체10.7%화13.1%,이급제고재300mg/L NaIO3조건하적생존솔체41.2%화37.7%.3mg/L유피소능제고HUVEC세포재200,4001xmol/L H2O2,조건하적생존솔체20.1%화21.5%.결론:유피소능구촉진ARPE-19세포적증식솔,억제HU-VEC생장,동시대저량충세포균유항양화작용.인차,유피소시치료노년황반변성적흔유전경적후선약물.
AIM: To evaluate the antioxidant activity of naringenin in human retinal pigment epithelium (ARPE-19) cells and human umbilical vein endothelial cells (HUVEC). ·METHODS: MTT assay was used to measure the viability and proliferation of ARPE-19 cells and HUVEC. ·RESULTS: Three and 10mg/L naringenin significantly increased the proliferation of ARPE-19 cells by 10.8% and 11.4%, respectively. Ten mg/L naringenin increased hypoxia-, 0.3mmol/L NAN3-, and 200μmol/L H2O2- induced damage of ARPE-19 cells by 55.2%, 69.2%, and 50.3%, respectively. One mg/L naringenin increased the viability of 50μmol/L t-BHP-, and 30mg/L NalO3-treated ARPE-19 cells by 20.2% and 30.4%, respectively. Thirty mg/L naringenin also increased the proliferation of 50μmol/L t-BHP-treated ARPE-19 cells by 32.2%, and 1mg/L naringenin increased the proliferation of 30, 100 and 300 mg/L NalO3-treated ARPE-19 cells by 30.3%, 10.3% and 18.5%, respectively. The reduction of HUVEC was 23.9%, 70.4% and 77.9% in the 3, 10 and 30mg/L naringenin-treated groups, respectively. Furthermore, 1 and 3mg/L naringenin increased hypoxia-induced damage in HUVEC by 10.7% and 13.1%, and 300mg/L NalO3- induced damage in HUVEC by 41.2% and 37.7%. Three mg/L naringenin increased 200 and 400μmol/L H2O2-in-jured HUVEC by 20.1% and 21.5%, respectively. ·CONCLUSION: Naringenin increases the proliferation of ARPE-19 cells and inhibites the growth of HUVEC, and has potent antioxidant activity in ARPE-19 cells and HUVEC.
