CAJ | 학술논문

26例患者中骨折延迟愈合14例,骨不连12例,均行骨膜下及骨折间隙注射成骨细胞治疗.从患者自身髂后上棘抽取骨髓组织,经体外诱导、培养、扩增为成骨细胞,按无菌操作术在X射线定位下于骨折延迟愈合或骨不连病变部位骨膜下及骨折间隙注射浓度为1×105cells/mL的成骨细胞5～8mL,注射后4,6,10,14周定期摄X射线片观察病变部位骨痂生长情况.所宵病例随访3～12个月,平均5.3个月.复查X射线片见4周有骨痂形成,6周骨痂包绕骨折断端,10周骨折线模糊,14周骨折线消失.所有患者骨折均愈合,平均愈合时间为12.1周.提示经皮骨膜下注射成骨细胞治疗骨折延迟愈合与骨不连是一种有效的修复方法.
26례환자중골절연지유합14례,골불련12례,균행골막하급골절간극주사성골세포치료.종환자자신가후상극추취골수조직,경체외유도、배양、확증위성골세포,안무균조작술재X사선정위하우골절연지유합혹골불련병변부위골막하급골절간극주사농도위1×105cells/mL적성골세포5～8mL,주사후4,6,10,14주정기섭X사선편관찰병변부위골가생장정황.소소병례수방3～12개월,평균5.3개월.복사X사선편견4주유골가형성,6주골가포요골절단단,10주골절선모호,14주골절선소실.소유환자골절균유합,평균유합시간위12.1주.제시경피골막하주사성골세포치료골절연지유합여골불련시일충유효적수복방법.
A total of 26 patients comprising 14 with delayed fracture healing and 12 with bone nonunion were treated via subperiosteum and fractured spatial injection of osteoblasts. Bone marrow was extracted from posterior superior lilac spine to obtain osteoblasts following in vitro induction, culture and amplification. Subsequently, 5-8 mL osteoblasts at a density of 1 ×105 cells/mL was sterilely injected into subperiosteum and fractured interspace in injured region using X-ray positioning. At 4, 6, 10 and 14 weeks after injection, callus formation was checked using X-ray. All cases followed up for 3-12 months, with the means of 5.3 months. Callus was formed after 4 weeks, fractured ends were wrapped up by callus after 6 weeks, and fracture line was unclear after 10 weeks and disappeared after 14 weeks. Bone fracture was healed, with the mean healing time of 12.1 weeks, suggesting that percutaneous subperiosteum injection of osteoblasts is an effective method to treat delayed fracture healing and bone nonunion.