上海交通大学学报（医学版）
上海交通大學學報（醫學版）
상해교통대학학보（의학판）
JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY(MEDICAL SCIENCE)
2009年
7期
825-827
,共3页
葛健%项行%徐建敏%周颖明%翟青%王玲
葛健%項行%徐建敏%週穎明%翟青%王玲
갈건%항행%서건민%주영명%적청%왕령
牛磺酸%角膜内皮细胞%细胞培养
牛磺痠%角膜內皮細胞%細胞培養
우광산%각막내피세포%세포배양
taurine%corneal endothelial cell%cell culture
目的 探讨牛磺酸对兔眼角膜内皮细胞的毒副作用.方法 取家兔6只(共12眼),每只眼制取6片组织片,用组织块培养法培养兔角膜内皮细胞,细胞接种于12孔培养板,分别加入2%、4%、6%、8%、10%的牛磺酸液(同一动物的左右眼加同一浓度牛磺酸溶液),设立空白对照.倒置显微镜观察角膜内皮细胞的生长情况;在培养的第1、2、4、6、8天,将部分培养细胞用Wright染色后观察细胞形态.结果 空白对照和加入2%、4%、6%牛磺酸溶液培养的角膜内皮细胞,1周后均形成内皮细胞层,细胞形态呈六角形或类圆形.加入8%牛磺酸液培养的角膜内皮细胞,在第4天时生长不良,细胞胞体小;第8天时培养细胞死亡.加入10%牛磺酸液培养的角膜内皮细胞,在第2天时出现生长不良,细胞胞体小,部分细胞已死亡.同一动物的左右眼角膜内皮细胞生长速度和细胞形态相似.结论 2%～6%牛磺酸对兔角膜内皮细胞的生长无不良影响,提示该浓度范围的牛磺酸对角膜内皮细胞无毒副作用.
目的 探討牛磺痠對兔眼角膜內皮細胞的毒副作用.方法 取傢兔6隻(共12眼),每隻眼製取6片組織片,用組織塊培養法培養兔角膜內皮細胞,細胞接種于12孔培養闆,分彆加入2%、4%、6%、8%、10%的牛磺痠液(同一動物的左右眼加同一濃度牛磺痠溶液),設立空白對照.倒置顯微鏡觀察角膜內皮細胞的生長情況;在培養的第1、2、4、6、8天,將部分培養細胞用Wright染色後觀察細胞形態.結果 空白對照和加入2%、4%、6%牛磺痠溶液培養的角膜內皮細胞,1週後均形成內皮細胞層,細胞形態呈六角形或類圓形.加入8%牛磺痠液培養的角膜內皮細胞,在第4天時生長不良,細胞胞體小;第8天時培養細胞死亡.加入10%牛磺痠液培養的角膜內皮細胞,在第2天時齣現生長不良,細胞胞體小,部分細胞已死亡.同一動物的左右眼角膜內皮細胞生長速度和細胞形態相似.結論 2%～6%牛磺痠對兔角膜內皮細胞的生長無不良影響,提示該濃度範圍的牛磺痠對角膜內皮細胞無毒副作用.
목적 탐토우광산대토안각막내피세포적독부작용.방법 취가토6지(공12안),매지안제취6편조직편,용조직괴배양법배양토각막내피세포,세포접충우12공배양판,분별가입2%、4%、6%、8%、10%적우광산액(동일동물적좌우안가동일농도우광산용액),설립공백대조.도치현미경관찰각막내피세포적생장정황;재배양적제1、2、4、6、8천,장부분배양세포용Wright염색후관찰세포형태.결과 공백대조화가입2%、4%、6%우광산용액배양적각막내피세포,1주후균형성내피세포층,세포형태정륙각형혹류원형.가입8%우광산액배양적각막내피세포,재제4천시생장불량,세포포체소;제8천시배양세포사망.가입10%우광산액배양적각막내피세포,재제2천시출현생장불량,세포포체소,부분세포이사망.동일동물적좌우안각막내피세포생장속도화세포형태상사.결론 2%～6%우광산대토각막내피세포적생장무불량영향,제시해농도범위적우광산대각막내피세포무독부작용.
Objective To investigate the adverse effects of taurine on rabbit corneal endothelial cells. Methods Six rabbits (12 eyes) were selected, and 6 histologic sections were prepared from each of the eyes. Rabbit corneal endothelial cells were cultured by explant culture method. Cells were innoculated on a 12-well tissue culture plate, 2%, 4%, 6%, 8% and 10% taurine solutions were added respectively (cells from the right and left eyes of the same rabbit were added the same concentration of taurine solution), and blank control was established. The growth of corneal endothelial cells was observed by inverted microscopy, and cell morphology on the 1st, 2nd, 4th, 6th and 8th day of culture was observed with Wright staining. Results Corneal endothelial cells cultured with 2%, 4% and 6% taurine solutions and those of blank control formed endothelial cell layers after culture for one week, and the cells exhibited hexagonal or round-like morphology. Corneal endothelial cells cultured with 8% taurine solution appeared to be undergrowth with small cell body on the 4th day, and cell death occurred on the 8th day. Corneal endothelial cells cultured with 10% taurine solution turned out to be undergrowth with small cell body on the 2nd day, and cell death had occurred. The same growth velocity and cell morphology were observed in the corneal endothelial cells from the right and left eyes of the same rabbit. Conclusion Taurine with concentration between 2% and 6% has no adverse effects on the growth of rabbit corneal endothelial cells.
