生物技术
生物技術
생물기술
BIOTECHNOLOGY
2009年
5期
67-69
,共3页
吴华昌%马钦元%邓静%石岩昌%由耀辉
吳華昌%馬欽元%鄧靜%石巖昌%由耀輝
오화창%마흠원%산정%석암창%유요휘
发酵液%透明质酸%分离提纯%超声波
髮酵液%透明質痠%分離提純%超聲波
발효액%투명질산%분리제순%초성파
broth%hyaluronic acid%separation and purification%ultrasonic wave
目的:提高发酵液中透明质酸的提取率.方法:应用超声波预处理发酵液的方法对醇沉法提取透明质酸的影响进行研究.结果:在超声波功率100W、处理温度15℃、处理时间10min时,透明质酸提取率相对于未经超声波处理的发酵液提高了38.6%.因此,超声波预处理透明质酸发酵液可以达到提高透明质酸提取率的目的.
目的:提高髮酵液中透明質痠的提取率.方法:應用超聲波預處理髮酵液的方法對醇沉法提取透明質痠的影響進行研究.結果:在超聲波功率100W、處理溫度15℃、處理時間10min時,透明質痠提取率相對于未經超聲波處理的髮酵液提高瞭38.6%.因此,超聲波預處理透明質痠髮酵液可以達到提高透明質痠提取率的目的.
목적:제고발효액중투명질산적제취솔.방법:응용초성파예처리발효액적방법대순침법제취투명질산적영향진행연구.결과:재초성파공솔100W、처리온도15℃、처리시간10min시,투명질산제취솔상대우미경초성파처리적발효액제고료38.6%.인차,초성파예처리투명질산발효액가이체도제고투명질산제취솔적목적.
Objective:Taking the improvement of the extraction rate.Method:The effect of purification of Hyaluronic acid from broth by ethanol precipitation method was investigated by pretreating the broth using ultrasonic wave.Result: For the untreated broth,the extraction rate of Hyaluronic acid from the treated broth was improved by 38.6% under the condition of ultrasonic wave frequency 40kHz ,ultrasonic time 10min,temperature 15℃ and ultrasonic power 100W.A conclusion could be got that the extraction rate could be improved from the broth pretreated by ultrasonic wave.
