肾脏病与透析肾移植杂志
腎髒病與透析腎移植雜誌
신장병여투석신이식잡지
CHINESE JOURNAL OF NEPHROLOGY,DIALYSIS & TRANSPLANTATION
2009年
6期
519-528
,共10页
高清%刘志红%秦卫松%郑春霞%张明超%曾彩虹%王生余%王建平%朱晓东%黎磊石
高清%劉誌紅%秦衛鬆%鄭春霞%張明超%曾綵虹%王生餘%王建平%硃曉東%黎磊石
고청%류지홍%진위송%정춘하%장명초%증채홍%왕생여%왕건평%주효동%려뢰석
雷公藤甲素%缬沙坦%db/db糖尿病小鼠%糖尿病%蛋白尿
雷公籐甲素%纈沙坦%db/db糖尿病小鼠%糖尿病%蛋白尿
뢰공등갑소%힐사탄%db/db당뇨병소서%당뇨병%단백뇨
triptolide%valsatan%db/db mice%diabetic nephropathy%albuminuria
目的:利用db/db小鼠验证雷公藤甲素对糖尿病肾脏损伤的治疗作用,探讨雷公藤甲素防治糖尿病肾病的机制. 方法:9周龄的db/db和db/m小鼠分为五组:(1)db/m正常对照组;(2)dh/dh糖尿病对照组;(3)缬沙坦治疗纽;(4)雷公藤甲素低剂最治疗组;(5)雷公藤甲素高剂繁治疗组.于4周、8周及12周测定各组24h尿白蛋白、血生化和体重.光镜观察肾小球病变,电镜观察足突改变,并作定量分析.免疫病理观察足细胞裂孔膜蛋白nephrin,足细胞损伤标志物desmin,炎症反应标志物单核细胞趋化蛋白1(MCP-1),氧化应激标志物4羟壬烯醛(4-HNE)的表达. 结果:db/db小鼠经雷公藤甲素治疗后蛋白尿下降,肾小球肥大和足细胞损伤减轻,肾组织炎症和氧化应激状态改善,同时高血脂和肥胖减轻.该作用随治疗时间延长,效果更加明显,且高剂量疗效优于低剂量.雷公藤甲素降低蛋白尿、改善肾小球肥大的作用与缬沙坦相似,但降低肾组织炎症和氧化应激的作用雷公藤甲素比缬沙坦更强. 结论:雷公藤甲索能明显降低dh/db小鼠尿蛋白的排泄,减轻肾组织炎症反应,改善肾脏组织病变,对糖尿病肾脏损伤具有显著且更全面的治疗作用.
目的:利用db/db小鼠驗證雷公籐甲素對糖尿病腎髒損傷的治療作用,探討雷公籐甲素防治糖尿病腎病的機製. 方法:9週齡的db/db和db/m小鼠分為五組:(1)db/m正常對照組;(2)dh/dh糖尿病對照組;(3)纈沙坦治療紐;(4)雷公籐甲素低劑最治療組;(5)雷公籐甲素高劑繁治療組.于4週、8週及12週測定各組24h尿白蛋白、血生化和體重.光鏡觀察腎小毬病變,電鏡觀察足突改變,併作定量分析.免疫病理觀察足細胞裂孔膜蛋白nephrin,足細胞損傷標誌物desmin,炎癥反應標誌物單覈細胞趨化蛋白1(MCP-1),氧化應激標誌物4羥壬烯醛(4-HNE)的錶達. 結果:db/db小鼠經雷公籐甲素治療後蛋白尿下降,腎小毬肥大和足細胞損傷減輕,腎組織炎癥和氧化應激狀態改善,同時高血脂和肥胖減輕.該作用隨治療時間延長,效果更加明顯,且高劑量療效優于低劑量.雷公籐甲素降低蛋白尿、改善腎小毬肥大的作用與纈沙坦相似,但降低腎組織炎癥和氧化應激的作用雷公籐甲素比纈沙坦更彊. 結論:雷公籐甲索能明顯降低dh/db小鼠尿蛋白的排洩,減輕腎組織炎癥反應,改善腎髒組織病變,對糖尿病腎髒損傷具有顯著且更全麵的治療作用.
목적:이용db/db소서험증뢰공등갑소대당뇨병신장손상적치료작용,탐토뢰공등갑소방치당뇨병신병적궤제. 방법:9주령적db/db화db/m소서분위오조:(1)db/m정상대조조;(2)dh/dh당뇨병대조조;(3)힐사탄치료뉴;(4)뢰공등갑소저제최치료조;(5)뢰공등갑소고제번치료조.우4주、8주급12주측정각조24h뇨백단백、혈생화화체중.광경관찰신소구병변,전경관찰족돌개변,병작정량분석.면역병리관찰족세포렬공막단백nephrin,족세포손상표지물desmin,염증반응표지물단핵세포추화단백1(MCP-1),양화응격표지물4간임희철(4-HNE)적표체. 결과:db/db소서경뢰공등갑소치료후단백뇨하강,신소구비대화족세포손상감경,신조직염증화양화응격상태개선,동시고혈지화비반감경.해작용수치료시간연장,효과경가명현,차고제량료효우우저제량.뢰공등갑소강저단백뇨、개선신소구비대적작용여힐사탄상사,단강저신조직염증화양화응격적작용뢰공등갑소비힐사탄경강. 결론:뢰공등갑색능명현강저dh/db소서뇨단백적배설,감경신조직염증반응,개선신장조직병변,대당뇨병신장손상구유현저차경전면적치료작용.
Objective:To investigate the effects and mechanisms of triptolide on renal lesions in db/db with mice diabetic nephropathy. Methodology: db/db and db/m mice with 9-wks of age were divided into five groups: group A, db/m mice were treated with saline; group B, db/db mice with saline; group C, db/db mice with valsatan [ 20 mg/(kg·d) ] ; group D and E, db/db mice with triptolide [25 μg/(kg·d) and 50 μg/(kg·d) ]. The body weight, levels of plasma glucose, plasma lipids, and 24-h urinary albumin excretion were measured every 4 weeks. The morphological changes of renal tissue, immunofluorescence and immunohistology of nephrin, desmin, MCP-1 and 4-HNE were detected in all groups. Results :The level of urinary albumin excretion, glomerular area, Glomerular/ Bowman's capsule volume ratio ( % ) , and foot process width were decreased in triptolide group. The podocyte density in triptolide group was higher than that in db/db control group. The expressions of nephrin, desmin, MCP-1 and 4-HNE were significantly improved by triptolide. The plasma levels of cholesterol, triglyceride, low density lipoprotein and high density lipoprotein were also improved by triptolide treatment. Those effects of triptolide were dose and time dependent. Triptolide had the similar effects on ameliorating albuminuria and glomerular hypertrophy, and more powerful effects on ameliorating inflammation and oxidative stress in renal tissue. Conclusion: Triptolide had significant and general renoprotective effects on db/db mice with diabetic nephropathy, which may be related with ameliorating albuminuria, relieving inflammation and improving renal lesion by triptolide.
