哈尔滨医科大学学报
哈爾濱醫科大學學報
합이빈의과대학학보
JOURNAL OF HARBIN MEDICAL UNIVERSITY
2010年
1期
1-4
,共4页
刘希君%孙博%王菁华%王丹丹%李呼伦%孔庆飞%白莎莎%刘玉梅%金连弘%王广友
劉希君%孫博%王菁華%王丹丹%李呼倫%孔慶飛%白莎莎%劉玉梅%金連弘%王廣友
류희군%손박%왕정화%왕단단%리호륜%공경비%백사사%류옥매%금련홍%왕엄우
实验性自身免疫性脑脊髓炎%骨髓基质干细胞%调节性T细胞%Th17
實驗性自身免疫性腦脊髓炎%骨髓基質榦細胞%調節性T細胞%Th17
실험성자신면역성뇌척수염%골수기질간세포%조절성T세포%Th17
experimental autoimmune encephalomyelitis%bone marrow stromal cells%Treg%Th17
目的 探讨骨髓基质干细胞(BMSCs) 对过继免疫实验性自身免疫性脑脊髓炎(AT-EAE) 的治疗作用及相关免疫调节机制.方法 分离纯化培养BMSCs,建立EAE 动物模型,在EAE动物模型发病高峰期取淋巴细胞,加入MBP刺激并与BMSCs共培养,并设未共培养的单纯MBP刺激对照组.将培养后的淋巴细胞对大鼠进行过继免疫回输,通过体外增殖实验,流式细胞仪检测以及体内临床评分评估BMSCs对EAE的治疗作用及其机制.结果 BMSCs共培养MBP组与MBP组相比,淋巴细胞增殖明显被抑制,CD4+Foxp3+细胞显著增高而CD4+IL-17+细胞显著降低,培养后的淋巴细胞进行过继免疫回输后,干细胞共培养组临床评分显著降低(P<0.05).结论 BMSCs通过调节免疫系统的调节性T细胞(Treg)和Th17的细胞平衡,即上调调节性T细胞分化和下调Th17细胞功能,对EAE起治疗作用.
目的 探討骨髓基質榦細胞(BMSCs) 對過繼免疫實驗性自身免疫性腦脊髓炎(AT-EAE) 的治療作用及相關免疫調節機製.方法 分離純化培養BMSCs,建立EAE 動物模型,在EAE動物模型髮病高峰期取淋巴細胞,加入MBP刺激併與BMSCs共培養,併設未共培養的單純MBP刺激對照組.將培養後的淋巴細胞對大鼠進行過繼免疫迴輸,通過體外增殖實驗,流式細胞儀檢測以及體內臨床評分評估BMSCs對EAE的治療作用及其機製.結果 BMSCs共培養MBP組與MBP組相比,淋巴細胞增殖明顯被抑製,CD4+Foxp3+細胞顯著增高而CD4+IL-17+細胞顯著降低,培養後的淋巴細胞進行過繼免疫迴輸後,榦細胞共培養組臨床評分顯著降低(P<0.05).結論 BMSCs通過調節免疫繫統的調節性T細胞(Treg)和Th17的細胞平衡,即上調調節性T細胞分化和下調Th17細胞功能,對EAE起治療作用.
목적 탐토골수기질간세포(BMSCs) 대과계면역실험성자신면역성뇌척수염(AT-EAE) 적치료작용급상관면역조절궤제.방법 분리순화배양BMSCs,건립EAE 동물모형,재EAE동물모형발병고봉기취림파세포,가입MBP자격병여BMSCs공배양,병설미공배양적단순MBP자격대조조.장배양후적림파세포대대서진행과계면역회수,통과체외증식실험,류식세포의검측이급체내림상평분평고BMSCs대EAE적치료작용급기궤제.결과 BMSCs공배양MBP조여MBP조상비,림파세포증식명현피억제,CD4+Foxp3+세포현저증고이CD4+IL-17+세포현저강저,배양후적림파세포진행과계면역회수후,간세포공배양조림상평분현저강저(P<0.05).결론 BMSCs통과조절면역계통적조절성T세포(Treg)화Th17적세포평형,즉상조조절성T세포분화화하조Th17세포공능,대EAE기치료작용.
Objective To investigate therapeutic effect and immunomodulation mechanism of bone marrow stem cells (BMSCs) treating adoptive transfer experimental autoimmune encephalomyelitis (AT-EAE). Methods BMSCs were purified and cultured, EAE model was established, T lymphocytes were isolated at the peak of disease. T cells were stimulated with MBP in the presence or absence of BMSCs, these cells were injected into the naive rat tail vein. The corresponding indexes of EAE were evaluated by lymphocyte proliferation assays and flow cytometry test in vitro, clinical assessment in vivo. Results Compared with MBP group, the proliferation of lymphocytes and clinical score of (BMSCs+MBP) group significantly decreased (P<0.05). The proportion of Treg increased while Th17 decreased. Conclusion BMSCs strongly reduce proliferative responses of lymphocytes of EAE rats, promote Treg generation and inhibit Th17 cell generation.
