CAJ | 학술논문

目的:研究锡类散对小鼠口腔成纤维细胞的影响,探索其促伤口愈合的机制.方法:采用MTT法、氯胺-T法、明胶酶谱分析和酶联免疫反应测定锡类散对小鼠口腔成纤维细胞的增殖、群体倍增时间、胶原、金属蛋白酶-2,9(MMP-2,9)、基质金属蛋白酶抑制因子-1(TIMP-1)的影响.结果:锡类散水提物能以时间和浓度依赖性方式促进小鼠口腔成纤维细胞生长,经水提物(125 μg/mL)处理后,细胞群体倍增时间为33.8 h,并且胶原积累显著增加,其水提物和醇提物都能显著(P＜0.05)增加MMP-2活性,同时极显著(P＜0.01)提高TIMP-1的合成.结论:锡类散通过促进成纤维细胞分裂、胶原沉积和TIMP-1的产生而激活成纤维细胞,促伤口愈合.
목적:연구석류산대소서구강성섬유세포적영향,탐색기촉상구유합적궤제.방법:채용MTT법、록알-T법、명효매보분석화매련면역반응측정석류산대소서구강성섬유세포적증식、군체배증시간、효원、금속단백매-2,9(MMP-2,9)、기질금속단백매억제인자-1(TIMP-1)적영향.결과:석류산수제물능이시간화농도의뢰성방식촉진소서구강성섬유세포생장,경수제물(125 μg/mL)처리후,세포군체배증시간위33.8 h,병차효원적루현저증가,기수제물화순제물도능현저(P＜0.05)증가MMP-2활성,동시겁현저(P＜0.01)제고TIMP-1적합성.결론:석류산통과촉진성섬유세포분렬、효원침적화TIMP-1적산생이격활성섬유세포,촉상구유합.
AIM:Xilei Powder,a traditional Chinese prescription,has been used to treat wounds for hundreds of years,but the mechanism has not been fully understood.METHODS:The effects of Xilei Powder on fibroblast proliferation,collagen accumulation,matrix metalloproteinases-2,9 ( MMP-2,9 ) activities and tissue inhibitor of metalloproteinase 1 (TIMP-1) production were investigated by MTT,chloramine T method,gelatin zymography and enzyme-linked immunosorbent assays ( ELISA),respectively.RESULTS: The aqueous extract of Xilei Powder significantly promoted fibroblasts proliferation in a time and concentration manner,the population doubling time (125 μg/mL) was 33.8 h,it also significantly (P ＜0.05 ) promoted collagen production.Both of the aqueous and alcoholic extracts could significantly ( P ＜ 0.05 ) increase MMPo2 activity,and also very significantly ( P ＜ 0.01 )promote TIMP-1 production.CONCLUSION: Xilei Powder could promote fibroblasts proliferation,collagen and TIMP-1 production,this might be parts of mechanism to promote wound healing.