时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
4期
792-793
,共2页
唐前%罗燕英%唐玲%黄连冬%于大永%冯宝民%王永奇
唐前%囉燕英%唐玲%黃連鼕%于大永%馮寶民%王永奇
당전%라연영%당령%황련동%우대영%풍보민%왕영기
金花茶种子%总多酚%提取工艺%正交实验
金花茶種子%總多酚%提取工藝%正交實驗
금화다충자%총다분%제취공예%정교실험
Theopsis chrysantha Hu.seeds%Total polyphenol%Extracting technology%Orthogonal test
目的 优选金花茶种子中总多酚的提取工艺.方法 采用丙酮-水法从金花茶种子中提取总多酚,利用正交实验优选总多酚最佳提取工艺,考察丙酮浓度、时间、温度、提取次数及料液比对总多酚收率的影响.结果 总多酚的最佳提取工艺为:25倍量的50%丙酮-水,80℃,提取3次,4 h/次,总多酚含量为9.59%,总多酚的收率为1.71%.结论 丙酮-水提取法可用于金花茶种子中总多酚的提取,其中提取溶剂倍量和提取温度对总多酚提取的影响较大.
目的 優選金花茶種子中總多酚的提取工藝.方法 採用丙酮-水法從金花茶種子中提取總多酚,利用正交實驗優選總多酚最佳提取工藝,攷察丙酮濃度、時間、溫度、提取次數及料液比對總多酚收率的影響.結果 總多酚的最佳提取工藝為:25倍量的50%丙酮-水,80℃,提取3次,4 h/次,總多酚含量為9.59%,總多酚的收率為1.71%.結論 丙酮-水提取法可用于金花茶種子中總多酚的提取,其中提取溶劑倍量和提取溫度對總多酚提取的影響較大.
목적 우선금화다충자중총다분적제취공예.방법 채용병동-수법종금화다충자중제취총다분,이용정교실험우선총다분최가제취공예,고찰병동농도、시간、온도、제취차수급료액비대총다분수솔적영향.결과 총다분적최가제취공예위:25배량적50%병동-수,80℃,제취3차,4 h/차,총다분함량위9.59%,총다분적수솔위1.71%.결론 병동-수제취법가용우금화다충자중총다분적제취,기중제취용제배량화제취온도대총다분제취적영향교대.
Objective To optimize the technology for extraction of total polyphenol from Theopsis chrysantha Hu.Seeds.Methods The seeds was extracted using acetone-aqueous,and the extract rate of total polyphenol was studied by orthogonal test,and the effects of solvent concentration of extraction,time of extraction,temperature of extraction,number times of extraction,solvent times of extraction were investigated.Results The optimum extraction technology was as follows: 25 times 50% acetone-aqueous,extracting 3 times at 80℃ for 4h once.By this technology the extraction rate of total polyphenol was 1.71% and the content of total polyphenol was 9.59%.Conclusion This method can be applied the extraction of total polyphenol in Theopsis chrysantha Hu.Seeds.The extracting solvent times and temperature of extraction show objective effects.
