CAJ | 학술논문

目的:评价组织工程修复股骨骨缺损各种生物材料的性能和应用,寻找合理股骨替代物.方法:以"组织工程,股骨缺损,干细胞,细胞因子,复合材料"为中文关键词,"tissue engineering,femur defect,Mesenchymal stem cells,Cytokines,Materials"为英文关键词,采用计算机检索1993-01/2009-10相关文章.纳入与有关生物材料与组织工程股骨缺损修复相关的文章;排除重复研究或Meta分析类文章.以21篇文献为主重点讨论了组织工程股骨骨缺损修复生物材料及其性能.结果:干细胞技术是从少量骨髓组织得到种子细胞,经体外诱导扩增达到一定细胞数量后,与支架材料复合,体外构建组织工程骨,其细胞能够维持成骨细胞特有的生物学特性,能与受区骨组织完全融合,同时避免了免疫排斥反应发生.将两种或两种以上材料复合在一起,或对生物材料表面进行各种各样的修饰,可以促进细胞与材料之间的黏附、提高细胞的生物活性并维持生物功能;利用某种载体转入到种子细胞内,后者在骨缺损区成骨,同时分泌适量的骨生长因子,诱导周围非定向性骨祖细胞向定向性骨祖细胞分化,从而在短时间内募集到足够的具有成骨活性的种子细胞,加快长骨损伤愈合;利用计算机三维虚拟成像技术和计算机数控成型技术预制的复合材料,既有医用复合材料本身的优点,又有精确的和股骨骨缺损周围组织解剖学上契合性,更有近乎完美的外观形态.结论:目前还没有一种材料能完全符合骨组织工程的要求.将几种材料复合在一起,或对生物材料表面修饰,促进细胞与材料之间的黏附、提高细胞的生物活性、维持生物功能是目前组织工程生物材料研究的热点.
목적:평개조직공정수복고골골결손각충생물재료적성능화응용,심조합리고골체대물.방법:이"조직공정,고골결손,간세포,세포인자,복합재료"위중문관건사,"tissue engineering,femur defect,Mesenchymal stem cells,Cytokines,Materials"위영문관건사,채용계산궤검색1993-01/2009-10상관문장.납입여유관생물재료여조직공정고골결손수복상관적문장;배제중복연구혹Meta분석류문장.이21편문헌위주중점토론료조직공정고골골결손수복생물재료급기성능.결과:간세포기술시종소량골수조직득도충자세포,경체외유도확증체도일정세포수량후,여지가재료복합,체외구건조직공정골,기세포능구유지성골세포특유적생물학특성,능여수구골조직완전융합,동시피면료면역배척반응발생.장량충혹량충이상재료복합재일기,혹대생물재료표면진행각충각양적수식,가이촉진세포여재료지간적점부、제고세포적생물활성병유지생물공능;이용모충재체전입도충자세포내,후자재골결손구성골,동시분비괄량적골생장인자,유도주위비정향성골조세포향정향성골조세포분화,종이재단시간내모집도족구적구유성골활성적충자세포,가쾌장골손상유합;이용계산궤삼유허의성상기술화계산궤수공성형기술예제적복합재료,기유의용복합재료본신적우점,우유정학적화고골골결손주위조직해부학상계합성,경유근호완미적외관형태.결론:목전환몰유일충재료능완전부합골조직공정적요구.장궤충재료복합재일기,혹대생물재료표면수식,촉진세포여재료지간적점부、제고세포적생물활성、유지생물공능시목전조직공정생물재료연구적열점.
OBJECTIVE: To evaluate the features and application of tissue engineered biomaterials in repairing femur defects, and to search an optimal femur substitution.METHODS: Papers published between January 1993 and October 2009 were searched using computer with key words of "tissue engineering, femur defect, mesenchymal stem cells, cytokines and materials" both in English and Chinese. Documents addressing biomaterials and tissue engineered bone in repairing femur defects were included. Repetitive research or Meta analysis was excluded. After that, 21 literatures were selected to discuss the features and application of tissue engineered biomaterials in repairing femur defects.RESULTS: Stem cells technology is obtaining seed cells from myeloid tissues, amplifying in vitro, combining with scaffold material, and in vitro constructing tissue engineered bones. The cultured cells could maintain osteoblast biological characteristics and fully fused with bone tissues at recipient sites, simultaneously, avoid immunological rejection. The combination of materials or modify the material surface could promote cells adhere to materials and increase the cellular biological activity. When transfecting seed cells to bone defect areas using certain carriers, the transplanted calls could form new bones and secret bone growth factor, induce determined osteogenic precursor cells differentiated into inducible ostegenic precursor cells, thereby, fasten the bone healing. The composite materials prepared by three-dimensional virtual surgical simulation and computer numerical control exhibited advantages of composite materials and histological anatomy conjunction, which had perfect appearance.CONCLUSION: Currently, there is no material can fully meet the requirement of bone tissue engineering. Thus, promoting adhesion between cells and materials, increasing cellular biological activity, and maintaining biological functions by combing materials or modifying material surface are the research focuses.