CAJ | 학술논문

目的制备肿瘤M2型-丙酮酸激酶(M2-PK)单克隆抗体,并建立免疫放射分析的检测方法.方法用肿瘤M2-PK抗原免疫小鼠,经细胞融合及筛选后得到一对单克隆抗体,建立免疫放射分析的方法,并评价该检测方法的灵敏度、特异度及精密度.结果获得了1A6和3F8两株效价高、特异度好的单克隆抗体,建立了双抗体夹心免疫放射分析方法.该检测方法的分析灵敏度为2 U/ml,临床灵敏度和特异度分别为70.5％和89.0％,提供的M2-PK的正常参考值范围是＜14.8 U/ml,批内不精密度＜5％,批间不精密度＜10％.结论建立的双抗体夹心免疫放射分析法可用于体检筛查及肿瘤患者的早期诊断,做到尽早治疗,提高患者的生存质量.
목적 제비종류M2형-병동산격매(M2-PK)단극륭항체,병건립면역방사분석적검측방법.방법 용종류M2-PK항원면역소서,경세포융합급사선후득도일대단극륭항체,건립면역방사분석적방법,병평개해검측방법적령민도、특이도급정밀도.결과 획득료1A6화3F8량주효개고、특이도호적단극륭항체,건립료쌍항체협심면역방사분석방법.해검측방법적분석령민도위2 U/ml,림상령민도화특이도분별위70.5％화89.0％,제공적M2-PK적정상삼고치범위시＜14.8 U/ml,비내불정밀도＜5％,비간불정밀도＜10％.결론 건립적쌍항체협심면역방사분석법가용우체검사사급종류환자적조기진단,주도진조치료,제고환자적생존질량.
Objective To produce monoclonal antibodies against tumor M2-type pyruvate kinase (M2-PK),and to establish immunoradiometric analysis for detection.Methods The BALB/c mice were immunized with M2-PK antigen.Monoclonal antibodies were generated after cell fusion and screening.Established detection method of immunoradiometric analysis and then evaluated its sensitivity,specificity and coefficient of variation.Results Two monoclonal antibodies ( 1A6 and 3F8) with high-titer and highspecificity were generated and detection method of immunoradiometric analysis was established.The analytical sensitivity of immunoradiometric analysis was 2 U/ml,while its clinical sensitivity and specificity was 70.5％ and 89.0％ respectively.The reference value of M2-PK level was less than 14.8 U/nd according to immunoradiometric analysis.Intra-assay coefficient of variation was less than 5％,while inter-assay coefficient of variation was less than 10％.Conclusion This sensitive immunoradiometric analysis can be used in early diagnosis of tumor and physical examination.