CAJ | 학술논문

目的探讨荧光标志物量子点在荧光原位杂交技术检测人宫颈癌组织中人乳头状瘤病毒16/18(HPV16/18)感染中的应用.方法以量子点荧光原位杂交(QD-FISH)和显色原位杂交方法(CISH)分别检测80例宫颈鳞癌活检组织中HPV16/18的感染情况,对其结果进行统计学分析.结果 QD-FISH检测宫颈鳞癌活检组织中HPV16/18阳性率为88.8%(71/80),高于CISH检出的阳性率(80%,64/80),但差异无统计学意义(P=0.127),并且HPV16/18感染的阳性率随着宫颈癌级别的上升而上升.结论 QD-FISH检测HPV感染的灵敏性和特异性均高于CISH,可作为筛查宫颈癌的一种方法.
목적 탐토형광표지물양자점재형광원위잡교기술검측인궁경암조직중인유두상류병독16/18(HPV16/18)감염중적응용.방법 이양자점형광원위잡교(QD-FISH)화현색원위잡교방법(CISH)분별검측80례궁경린암활검조직중HPV16/18적감염정황,대기결과진행통계학분석.결과 QD-FISH검측궁경린암활검조직중HPV16/18양성솔위88.8%(71/80),고우CISH검출적양성솔(80%,64/80),단차이무통계학의의(P=0.127),병차HPV16/18감염적양성솔수착궁경암급별적상승이상승.결론 QD-FISH검측HPV감염적령민성화특이성균고우CISH,가작위사사궁경암적일충방법.
Objective To investigate fluorescence in situ hybridization labeled with quantum dots (QDs) for the detection of human papillomavirus 16/18 (HPV16/18) infection in cervical carcinoma patients. Methods A total of 80 biopsy samples of squamous carcinoma of cervix were assayed for HPV 16/18 infection by using quantum dot labeled fluorescent in situ hybridization (QD-FISH) and chromogenic in situ hybridization (CISH) techniques, respectively. The results obtained by using two different methods were statistically analyzed. Results The positive rate for HPV16/18 by QD-FISH was 88.8% (71/80),higher than that (80. 0% ) by CISH, however, the result was statistically not significant (P =0. 127). The positive detection rates for HPV16/18 by using both methods increased coincidentally with raising of the tumor grading stage. Conclusion The sensitivity and specificity of HPV infection detectable by QD-FISH is higher than that by the CISH technique.