中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2010年
4期
240-244
,共5页
乳腺肿瘤%血管内皮生长因子C%基质金属蛋白酶类%RNA干扰
乳腺腫瘤%血管內皮生長因子C%基質金屬蛋白酶類%RNA榦擾
유선종류%혈관내피생장인자C%기질금속단백매류%RNA간우
Breast neoplasms%Vascular endothelial growth factor C%Marx metalloproteinases%RNA interference
目的 研究血管内皮生长因子C(VEGF-C)与基质金属蛋白酶(MMP)-2、MMP-9在乳腺癌组织中的表达及三者在乳腺癌淋巴结转移中的作用.方法 应用免疫组织化学SP法对84例乳腺癌(有腋淋巴结转移者52例,无腋淋巴结转移者32例)的VEGF-C、MMP-2和-9以及血管内皮透明质酸受体-1(LYVE-1)的表达进行检测,统计分析VEGF-C、MMP-2和-9与乳腺癌淋巴管生成的关系.利用重组质粒表达载体介导的短发夹式干扰RNA(shRNA)靶向沉默乳腺癌MCF-7细胞株中VEGF-C基因,PCR检测VEGF-C、MMP-2和-9的表达.结果 VEGF-C与MMP-2和-9在乳腺癌组织中均呈过表达,分别为83.3%(70/84)、89.3%(75/84)和75.0%(63/84),且在腋淋巴结转移组[94.2%(49/52)、98.1%(51/52)和88.5%(46/52)]比无淋巴结转移组[65.6%(21/32)、75.0%(24/32)和53.1%(17/32)]明显高表达(P＜0.05);淋巴管密度在腋淋巴结转移组及无转移组的表达有统计学意义(P＜0.05),随着VEGF-C与MMP-2和-9表达强度增加,淋巴管数量也增加(P＜0.05);转染重组质粒后MCF-7细胞株的VEGF-C及MMP-2和-9的mRNA表达水平下调,抑制率分别为95.0%、53.0%和77.0%(P＜0.05).结论 VEGF-C与MMP-2和-9协同促进乳腺癌组织的淋巴管新生和乳腺癌淋巴结转移.
目的 研究血管內皮生長因子C(VEGF-C)與基質金屬蛋白酶(MMP)-2、MMP-9在乳腺癌組織中的錶達及三者在乳腺癌淋巴結轉移中的作用.方法 應用免疫組織化學SP法對84例乳腺癌(有腋淋巴結轉移者52例,無腋淋巴結轉移者32例)的VEGF-C、MMP-2和-9以及血管內皮透明質痠受體-1(LYVE-1)的錶達進行檢測,統計分析VEGF-C、MMP-2和-9與乳腺癌淋巴管生成的關繫.利用重組質粒錶達載體介導的短髮夾式榦擾RNA(shRNA)靶嚮沉默乳腺癌MCF-7細胞株中VEGF-C基因,PCR檢測VEGF-C、MMP-2和-9的錶達.結果 VEGF-C與MMP-2和-9在乳腺癌組織中均呈過錶達,分彆為83.3%(70/84)、89.3%(75/84)和75.0%(63/84),且在腋淋巴結轉移組[94.2%(49/52)、98.1%(51/52)和88.5%(46/52)]比無淋巴結轉移組[65.6%(21/32)、75.0%(24/32)和53.1%(17/32)]明顯高錶達(P＜0.05);淋巴管密度在腋淋巴結轉移組及無轉移組的錶達有統計學意義(P＜0.05),隨著VEGF-C與MMP-2和-9錶達彊度增加,淋巴管數量也增加(P＜0.05);轉染重組質粒後MCF-7細胞株的VEGF-C及MMP-2和-9的mRNA錶達水平下調,抑製率分彆為95.0%、53.0%和77.0%(P＜0.05).結論 VEGF-C與MMP-2和-9協同促進乳腺癌組織的淋巴管新生和乳腺癌淋巴結轉移.
목적 연구혈관내피생장인자C(VEGF-C)여기질금속단백매(MMP)-2、MMP-9재유선암조직중적표체급삼자재유선암림파결전이중적작용.방법 응용면역조직화학SP법대84례유선암(유액림파결전이자52례,무액림파결전이자32례)적VEGF-C、MMP-2화-9이급혈관내피투명질산수체-1(LYVE-1)적표체진행검측,통계분석VEGF-C、MMP-2화-9여유선암림파관생성적관계.이용중조질립표체재체개도적단발협식간우RNA(shRNA)파향침묵유선암MCF-7세포주중VEGF-C기인,PCR검측VEGF-C、MMP-2화-9적표체.결과 VEGF-C여MMP-2화-9재유선암조직중균정과표체,분별위83.3%(70/84)、89.3%(75/84)화75.0%(63/84),차재액림파결전이조[94.2%(49/52)、98.1%(51/52)화88.5%(46/52)]비무림파결전이조[65.6%(21/32)、75.0%(24/32)화53.1%(17/32)]명현고표체(P＜0.05);림파관밀도재액림파결전이조급무전이조적표체유통계학의의(P＜0.05),수착VEGF-C여MMP-2화-9표체강도증가,림파관수량야증가(P＜0.05);전염중조질립후MCF-7세포주적VEGF-C급MMP-2화-9적mRNA표체수평하조,억제솔분별위95.0%、53.0%화77.0%(P＜0.05).결론 VEGF-C여MMP-2화-9협동촉진유선암조직적림파관신생화유선암림파결전이.
Objective To study the expression of matrix metalloproteinase(MMP)-2,MMP-9 and vascular endothelial growth factor(VEGF)-C in breast cancer and their role in lymph node metastasis.Methods Immunohistochemical staining was used to detect the expression of VEGF-C,MMP-2,MMP-9 and LYVE-1 in 84 cases of breast cancer.including 52 cases with and 32 cases without lymph node metastases.The recombinant vector(pSIREN-VEGF-C)was transfected into human breast cancer cell MCF-7 by liposome,and the RNA expression of MMP-2,MMP-9 and VEGF-C in MCF-7 cells after transfection was detected by PCR.Results The expressions of MMP-2,MMP-9 and VEGF-C were 98.1%(51/52),88.5%(46/52),and 94.2%(49/52)respectively for the metastatic group,and 75.0%(24/32),53.1%(17/32),and 65.6%(21/32)respectively for the non metastatic group,and there was significant difference between these groups (P＜0.05).The lymphatic vessel density between these two groups was also significantly different(P＜0.05).Increased expression of MMP-2,MMP-9 and VEGF-C was also associated with increased number of lymphatic vessels had also increased(P＜0.05).The expression of MMP-2,MMP-9 and VEGF-C in MCF-7 cells after gene transfection decreased significantly(P＜0.05).Conclusion MMP-2 and MMP-9 in conjunction with VEGF-C,promote lymphangiogenesis and lymph node metastasis of breast cancer.
