中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2011年
10期
829-832
,共4页
陈顺平%刘伟%余英豪%谢飞来%熊喜生%陈惠春%李敏
陳順平%劉偉%餘英豪%謝飛來%熊喜生%陳惠春%李敏
진순평%류위%여영호%사비래%웅희생%진혜춘%리민
乳腺肿瘤%基因,erbB-2%原位杂交,荧光%免疫组织化学
乳腺腫瘤%基因,erbB-2%原位雜交,熒光%免疫組織化學
유선종류%기인,erbB-2%원위잡교,형광%면역조직화학
Breast neoplasms%Genes,erbB-2%In situ hybridization,fluorescence%Immunohistochemistry
目的 了解HER-2基因与蛋白在乳腺癌中表达状况,探讨HER-2基因与Ki-67、P53、ER、PR、年龄、淋巴结转移、TNM分期的相关性及其临床意义.方法 收集150例浸润性乳腺癌组织石蜡标本,用荧光原位杂交( FISH)技术和免疫组化(IHC)法分别检测标本中的HER-2基因及HER-2、Ki-67、P53、ER、PR蛋白表达情况.结果 150例浸润性乳腺癌标本HER-2基因扩增率为32.68％ (49/150)；其中,在HER-2蛋白(+++)的病例中HER-2基因扩增率为90.32％ (28/31)；HER-2蛋白(++)的病例中扩增率34.78％ (16/46)；HER-2蛋白(+)和(-)的扩增率分别为11.54％(3/26)、4.25％(2/47).HER-2基因与ER、PR表达及TNM分期相关,而与年龄、Ki-67、P53、淋巴结转移无关.结论 FISH技术和IHC技术检测HER-2基因和蛋白表达在(+++)和(-)的病例中一致性较好,IHC可作为替代FISH检测HER-2基因状态的方法.
目的 瞭解HER-2基因與蛋白在乳腺癌中錶達狀況,探討HER-2基因與Ki-67、P53、ER、PR、年齡、淋巴結轉移、TNM分期的相關性及其臨床意義.方法 收集150例浸潤性乳腺癌組織石蠟標本,用熒光原位雜交( FISH)技術和免疫組化(IHC)法分彆檢測標本中的HER-2基因及HER-2、Ki-67、P53、ER、PR蛋白錶達情況.結果 150例浸潤性乳腺癌標本HER-2基因擴增率為32.68％ (49/150)；其中,在HER-2蛋白(+++)的病例中HER-2基因擴增率為90.32％ (28/31)；HER-2蛋白(++)的病例中擴增率34.78％ (16/46)；HER-2蛋白(+)和(-)的擴增率分彆為11.54％(3/26)、4.25％(2/47).HER-2基因與ER、PR錶達及TNM分期相關,而與年齡、Ki-67、P53、淋巴結轉移無關.結論 FISH技術和IHC技術檢測HER-2基因和蛋白錶達在(+++)和(-)的病例中一緻性較好,IHC可作為替代FISH檢測HER-2基因狀態的方法.
목적 료해HER-2기인여단백재유선암중표체상황,탐토HER-2기인여Ki-67、P53、ER、PR、년령、림파결전이、TNM분기적상관성급기림상의의.방법 수집150례침윤성유선암조직석사표본,용형광원위잡교( FISH)기술화면역조화(IHC)법분별검측표본중적HER-2기인급HER-2、Ki-67、P53、ER、PR단백표체정황.결과 150례침윤성유선암표본HER-2기인확증솔위32.68％ (49/150)；기중,재HER-2단백(+++)적병례중HER-2기인확증솔위90.32％ (28/31)；HER-2단백(++)적병례중확증솔34.78％ (16/46)；HER-2단백(+)화(-)적확증솔분별위11.54％(3/26)、4.25％(2/47).HER-2기인여ER、PR표체급TNM분기상관,이여년령、Ki-67、P53、림파결전이무관.결론 FISH기술화IHC기술검측HER-2기인화단백표체재(+++)화(-)적병례중일치성교호,IHC가작위체대FISH검측HER-2기인상태적방법.
Objective To study the amplification and expression of HER-2 gene and protein in breast cancer and to investigate the relationship between HER-2 gene and Ki-67 、P53 、ER、PR、lymph node metastasis and TNM staging. Methods Fluorescence in situ hybridization (FISH) and immunohistochemistry(IHC) method were used to detect the amplification and expression of HER-2 and the expression of Ki-67、P53、ER、PR in 150 cases of breast cancer. Results 49 out of 150 cases were amplificated,positive rate of HER-2 gene was 32.68％.In the 32 patients whose expression of HER-2 protein was ( + + + ),HER-2 gene was expressed in 28 cases.In The 46 patients whose expression of HER-2 protein was ( + + ),HER-2 was expressed in 16 cases.In the 26 patients whose expression of HER-2 protein was ( + ),HER-2 was expressed in 3 cases.In the 47 cases that the expression of HER-2 protein was negative,there were 45 cases that the expression HER-2 gene was negative.The expression of HER-2 was correlated with ER,PR expression and TNM staging,but not significantly related with age,Ki-67,P53 and lymph node metastasis.Conclusions FISH and IHC correlate well with each other in the determination of HER-2 gene amplication and protein expression in cases of invasive breast cancer.
