中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2010年
11期
780-783
,共4页
石磊%陈晓栋%杨圣菊%顾黎雄%孟国梁
石磊%陳曉棟%楊聖菊%顧黎雄%孟國樑
석뢰%진효동%양골국%고려웅%맹국량
瘢痕疙瘩%糖基化终产物,高级%受体
瘢痕疙瘩%糖基化終產物,高級%受體
반흔흘탑%당기화종산물,고급%수체
Keloid%Glycosylation end products,advanced%Receptor
目的 研究晚期糖基化终末产物(AGE)及其受体(AGER)在瘢痕疙瘩中的表达.方法 瘢痕疙瘩、增生性瘢痕和正常人群血清、皮肤组织标本各20份,以荧光分光光度计检测三组人群血清中AGE含量,分别采用免疫组化法、Western印迹分析检测三组人群皮肤组织标本中AGE和AGER表达情况.结果 瘢痕疙瘩组血清中AGE含量为(0.713±0.098)AU/ml,增生性瘢痕组为(0.699±0.077)AU/ml,明显高于正常人群组(0.179±0.056)AU/ml,三组差异有统计学意义(F=283.82,P<0.01).免疫组化显示,AGE、AGER在瘢痕疙瘩、增生性瘢痕皮肤组织标本中表达阳性,正常人群组织表达则为阴性.Western印迹检测显示,瘢痕疙瘩、增生性瘢痕组织中AGE和AGER蛋白表达均明显高于正常人群,差异有统计学意义(F值分别为18.04、42.80,P<0.05),而瘢痕疙瘩和增生性瘢痕组之间差异无统计学意义(P>0.05).结论 AGE和AGER在瘢痕疙瘩中表达升高,在其发病过程中可能发挥一定的促进作用.
目的 研究晚期糖基化終末產物(AGE)及其受體(AGER)在瘢痕疙瘩中的錶達.方法 瘢痕疙瘩、增生性瘢痕和正常人群血清、皮膚組織標本各20份,以熒光分光光度計檢測三組人群血清中AGE含量,分彆採用免疫組化法、Western印跡分析檢測三組人群皮膚組織標本中AGE和AGER錶達情況.結果 瘢痕疙瘩組血清中AGE含量為(0.713±0.098)AU/ml,增生性瘢痕組為(0.699±0.077)AU/ml,明顯高于正常人群組(0.179±0.056)AU/ml,三組差異有統計學意義(F=283.82,P<0.01).免疫組化顯示,AGE、AGER在瘢痕疙瘩、增生性瘢痕皮膚組織標本中錶達暘性,正常人群組織錶達則為陰性.Western印跡檢測顯示,瘢痕疙瘩、增生性瘢痕組織中AGE和AGER蛋白錶達均明顯高于正常人群,差異有統計學意義(F值分彆為18.04、42.80,P<0.05),而瘢痕疙瘩和增生性瘢痕組之間差異無統計學意義(P>0.05).結論 AGE和AGER在瘢痕疙瘩中錶達升高,在其髮病過程中可能髮揮一定的促進作用.
목적 연구만기당기화종말산물(AGE)급기수체(AGER)재반흔흘탑중적표체.방법 반흔흘탑、증생성반흔화정상인군혈청、피부조직표본각20빈,이형광분광광도계검측삼조인군혈청중AGE함량,분별채용면역조화법、Western인적분석검측삼조인군피부조직표본중AGE화AGER표체정황.결과 반흔흘탑조혈청중AGE함량위(0.713±0.098)AU/ml,증생성반흔조위(0.699±0.077)AU/ml,명현고우정상인군조(0.179±0.056)AU/ml,삼조차이유통계학의의(F=283.82,P<0.01).면역조화현시,AGE、AGER재반흔흘탑、증생성반흔피부조직표본중표체양성,정상인군조직표체칙위음성.Western인적검측현시,반흔흘탑、증생성반흔조직중AGE화AGER단백표체균명현고우정상인군,차이유통계학의의(F치분별위18.04、42.80,P<0.05),이반흔흘탑화증생성반흔조지간차이무통계학의의(P>0.05).결론 AGE화AGER재반흔흘탑중표체승고,재기발병과정중가능발휘일정적촉진작용.
Objective To investigate the expressions of advanced glycation end products (AGEs) and their receptors in keloid. Methods Serum and skin tissue specimens were collected from 20 patients with keloid, 20 patients with hyperplastic scar and 20 normal human controls. Fluorospectrophotometer was used to measure the serum level of AGEs, and immunohistochemistry and Western blotting to detect the expressions of AGEs and AGER in skin tissue specimens. Results The serum level of AGEs was (0.713 ± 0.098) AU/ml and (0.699 ± 0.077) AU/ml respectively in patients with keloid and those with hypertrophic scar, significantly higher than that in normal controls (0.179 ± 0.056 AU/ml, F = 283.82, P < 0.01 ). A positive expression of AGEs and AGER was observed in tissue specimens of keloid and hyperplastic scar, but not in the control specimens. As Western blotting showed, the expressions of AGEs and AGER were higher in tissue specimens of keloid and hyperplastic scar than in the control specimens (F = 18.04, 42.80, both P < 0.05), while no significant difference between keloid and hyperplastic scar tissue specimens was observed (P> 0.05). Conclusion There is a high expression of AGEs and AGER in keloid, which may contribute to the development of keloid.
