中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2012年
3期
263-265
,共3页
孙克康%赵琳%徐加英%焦旸
孫剋康%趙琳%徐加英%焦旸
손극강%조림%서가영%초양
肺癌%电离辐射%顺铂%erbB2转录因子1
肺癌%電離輻射%順鉑%erbB2轉錄因子1
폐암%전리복사%순박%erbB2전록인자1
Lung cancers%Ionizing radiations%Cisplatin%Transducer of erbB2. 1
目的 探讨电离辐射及其联合顺铂对人肺腺癌细胞株SPCA-1、LTEP-α-2中TOB1基因表达的影响.方法 体外培养人肺腺癌SPCA-1、LTEP-α-2细胞,用X射线一次性照射,细胞吸收剂量为2、4、6、8和10 Gy,源靶距为100 cm,吸收剂量率为200 cGy/min,在6GyX射线照射后6、12、18和24 h取样；X射线和顺铂联合对TOB1表达的研究设立单纯照射组(6 Gy)、顺铂处理组(20 mol/L顺铂)和联合处理组(6 Gy +20 mol/L顺铂)；上述实验同时设立未处理的对照组.采用RT-PCR和Western blot法检测TOB1 mRNA及蛋白表达水平的变化.结果 SPCA-1、LTEP-α-2细胞在不同剂量的X射线照射后24 h,其TOB1蛋白及mRNA表达量均明显增加(t=8.25 ～24.48,P ＜0.05)；不同时间检测提示,细胞在X射线照射后6h,TOB1蛋白及mRNA的表达量即开始上升,并持续到照射后24 h(t=14.23 ～15.82,P＜0.05)；联合处理组TOB1的表达量均明显高于单纯照射组、顺铂处理组和对照组(t=4.67 ～ 13.67,P＜0.05).结论 TOB1基因有可能作为临床肺癌放化疗靶基因,对临床放化疗疗效的判断具有一定的应用潜力.
目的 探討電離輻射及其聯閤順鉑對人肺腺癌細胞株SPCA-1、LTEP-α-2中TOB1基因錶達的影響.方法 體外培養人肺腺癌SPCA-1、LTEP-α-2細胞,用X射線一次性照射,細胞吸收劑量為2、4、6、8和10 Gy,源靶距為100 cm,吸收劑量率為200 cGy/min,在6GyX射線照射後6、12、18和24 h取樣；X射線和順鉑聯閤對TOB1錶達的研究設立單純照射組(6 Gy)、順鉑處理組(20 mol/L順鉑)和聯閤處理組(6 Gy +20 mol/L順鉑)；上述實驗同時設立未處理的對照組.採用RT-PCR和Western blot法檢測TOB1 mRNA及蛋白錶達水平的變化.結果 SPCA-1、LTEP-α-2細胞在不同劑量的X射線照射後24 h,其TOB1蛋白及mRNA錶達量均明顯增加(t=8.25 ～24.48,P ＜0.05)；不同時間檢測提示,細胞在X射線照射後6h,TOB1蛋白及mRNA的錶達量即開始上升,併持續到照射後24 h(t=14.23 ～15.82,P＜0.05)；聯閤處理組TOB1的錶達量均明顯高于單純照射組、順鉑處理組和對照組(t=4.67 ～ 13.67,P＜0.05).結論 TOB1基因有可能作為臨床肺癌放化療靶基因,對臨床放化療療效的判斷具有一定的應用潛力.
목적 탐토전리복사급기연합순박대인폐선암세포주SPCA-1、LTEP-α-2중TOB1기인표체적영향.방법 체외배양인폐선암SPCA-1、LTEP-α-2세포,용X사선일차성조사,세포흡수제량위2、4、6、8화10 Gy,원파거위100 cm,흡수제량솔위200 cGy/min,재6GyX사선조사후6、12、18화24 h취양；X사선화순박연합대TOB1표체적연구설립단순조사조(6 Gy)、순박처리조(20 mol/L순박)화연합처리조(6 Gy +20 mol/L순박)；상술실험동시설립미처리적대조조.채용RT-PCR화Western blot법검측TOB1 mRNA급단백표체수평적변화.결과 SPCA-1、LTEP-α-2세포재불동제량적X사선조사후24 h,기TOB1단백급mRNA표체량균명현증가(t=8.25 ～24.48,P ＜0.05)；불동시간검측제시,세포재X사선조사후6h,TOB1단백급mRNA적표체량즉개시상승,병지속도조사후24 h(t=14.23 ～15.82,P＜0.05)；연합처리조TOB1적표체량균명현고우단순조사조、순박처리조화대조조(t=4.67 ～ 13.67,P＜0.05).결론 TOB1기인유가능작위림상폐암방화료파기인,대림상방화료료효적판단구유일정적응용잠력.
Objective To explore the effect of ionizing radiation and cisplatin on the expression of Transducer of erbB2,1 ( TOB1 ) in human lung adenocareinoma SPCA-1 and LTEP-α-2 cell lines.Methods SPCA-1 and/or LTEP-α-2 cells were respectively irradiated with 2,4,6,8,and 10 Gy X-rays generated by a linear accelerator (with the source skin distance of 100 cm and dose rate of 200 cGy/min).The cell molecular samples were subtracted at 6,12,18,and 24 h after 6 Gy irradiation.For X-rays and cisplatin combination treatment,cells were divided into radiation group (6 Gy X-rays),cisplatin group (20 mol/L cisplatin),and combination group (6 Gy X-rays + 20 mol/L cisplatin).SPCA-1 and LTEP-α-2 cells without any treatment were used as control group.The relative levels of TOB1 mRNA and protein expression were detected by rigorously controlled semi-quantitative RT-PCR and Western blot analysis with quantitation of the mRNA/protein bands by densitometry.Results The expression level of TOB1 was significantly increased in both mRNA and protein level after radiation exposure (t =8.25-24.48,P ＜0.05).For time-dependent induction of TOB1,the expression was significantly increased 6 h after X-rays exposure (t =14.23-15.82,P ＜ 0.05 ).More significant increase of TOB1 expression was identified after the combination treatment of X-rays and cisplatin,compared to that of the radiation and/or cisplatin treatment alone (t =11.21-13.67,P ＜0.05).Conclusions lonizing radiation and cisplatin could upregulate the expression of TOB1 in lung cancer cells in both mRNA and protein levels.TOB1 may be a molecular target for ionizing radiation and cisplatin treatment in lung cancer and it may have potential implication in evaluating the curative efficiency of chemo-and radio-therapy.
