CAJ | 학술논문

目的观察胃转流术(GBP)对糖尿病大鼠血糖的控制效果及胰高血糖素样肽-1(GLP-1)的影响.方法采用链脲佐菌素建立糖尿病SD大鼠模型20只,随机分为糖尿病手术组(DO组)和糖尿病对照组(DC组),另取20只非糖尿病大鼠随机分为正常对照组(NC组)和正常手术组(NO组).分别检测各组大鼠术前、术后72 h、1周、4周和8周空腹血糖水平以及血清GLP-1浓度.结果术前DO组与DC组以及NC组与NO组大鼠空腹血糖之间的比较差异均无统计学意义(P＞0.05)；DO组大鼠术后空腹血糖进行性下降,术后8周由术前的(20.84±1.98) mmol/L下降到(5.56±0.11) mmol/L(P＜0.05)；DC组大鼠术前及术后各时相的差异无统计学意义(P＞0.05).DO组和NO组大鼠术后血清GLP-1浓度出现明显升高(P＜0.05),术后8周分别由术前的(7.10±0.55)、(10.73 ±0.67) pmol/L上升到(26.48±1.14)、(13.98±0.92) pmol/L(P＜0.05).结论 GBP对2型糖尿病大鼠具有明显的降糖作用,GLP-1的升高在其中起着重要作用,但对正常大鼠血糖无影响.
목적 관찰위전류술(GBP)대당뇨병대서혈당적공제효과급이고혈당소양태-1(GLP-1)적영향.방법 채용련뇨좌균소건립당뇨병SD대서모형20지,수궤분위당뇨병수술조(DO조)화당뇨병대조조(DC조),령취20지비당뇨병대서수궤분위정상대조조(NC조)화정상수술조(NO조).분별검측각조대서술전、술후72 h、1주、4주화8주공복혈당수평이급혈청GLP-1농도.결과 술전DO조여DC조이급NC조여NO조대서공복혈당지간적비교차이균무통계학의의(P＞0.05)；DO조대서술후공복혈당진행성하강,술후8주유술전적(20.84±1.98) mmol/L하강도(5.56±0.11) mmol/L(P＜0.05)；DC조대서술전급술후각시상적차이무통계학의의(P＞0.05).DO조화NO조대서술후혈청GLP-1농도출현명현승고(P＜0.05),술후8주분별유술전적(7.10±0.55)、(10.73 ±0.67) pmol/L상승도(26.48±1.14)、(13.98±0.92) pmol/L(P＜0.05).결론 GBP대2형당뇨병대서구유명현적강당작용,GLP-1적승고재기중기착중요작용,단대정상대서혈당무영향.
Objective To investigate the effect of gastric bypass surgery (GBP) on fasting bloodglucose (FBG) and glucagon-like peptide-1 (GLP-1) in type 2 diabetic rats.Methods The models of type 2 diabetic rats were induced by stretozotocin and 20 diabetic rats were randomly divided into two groups:diabetes-operation group ( DO group,n =10) and diabetes-control group ( DC group,n =10).Another twenty normal rats were randomly divided into two groups:normal-operation ( NO group,n =10)and normal-control group ( NC group,n =10).The FBG levels and glucagon-like peptide-1 ( GLP-1 ) concentrations of rats in each group were detected before operation and 72 h,on 1 week,4 weeks,8 weeks after operation.Results The FBG levels of rats before operation were not statistically significant different between DO group and DC group or between NO group and NC group ( P ＞ 0.05 ).After operation,the FBG levels of rats in DO group gradually declined,eight weeks after operation the FBG levels was declined from ( 20.84 ± 1.98 ) mmol/L to (5.56 ± 0.11 ) mmol/L ( P ＜ 0.05 ) ; In DC group,the difference of FBG levels of rats at different time point was not statistically significant (P ＞ 0.05).After operation,the concentrations of serum GLP-1 of rats in DO group and NO group gradually increased,eight weeks after operation The serum GLP-1 elevated respectively from (7.10 ± 0.55 ),( 10.73 ± 0.67) pmol/L to ( 26.48 ±1.14),( 13.98 ±0.92) pmol/L (P ＜0.05).Conclusion There is obvious hypoglycemic effect of GBP on FBG levels of type 2 diabetic rats other than normal rats,in which high secretion of GLP-1 may be play an important role,high secretion of GLP-1 on the normal type 2 diabetic rats has no hypoglycemic effect.