中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
2期
129-135
,共7页
王丽丽%龚凤云%谢旭华%陈佳%宋莹%夏超%申爱霞%邢铭友%许东%宋建新
王麗麗%龔鳳雲%謝旭華%陳佳%宋瑩%夏超%申愛霞%邢銘友%許東%宋建新
왕려려%공봉운%사욱화%진가%송형%하초%신애하%형명우%허동%송건신
铜绿假单胞菌%pvdQ基因%群集游动%抗生素耐药
銅綠假單胞菌%pvdQ基因%群集遊動%抗生素耐藥
동록가단포균%pvdQ기인%군집유동%항생소내약
Pseudomonas aeruginosa%pvdQ gene%Swarming motility%Antibiotic resistance
目的 探讨铜绿假单胞菌pvdQ(PA2385)基因在群集游动中对几种常用抗生素抗性的影响.方法 构建pvdQ基因表达质粒pME6032-pvdQ并鉴定,采用电穿孔法将带有pvdQ基因的质粒转入PAO1中,构建pvdQ高表达株.同时将空质粒pME6032采用电穿孔法转入PAO1中,构建pME6032空质粒株.在不同浓度抗生素中,通过比较群集游动直径的大小,观察PAO1和pvdQ高表达株对抗生素抗性的改变.结果 经鉴定成功构建pvdQ高表达株,比较两株菌群集游动直径大小:抗生素浓度成倍增加,但群集游动直径不是成倍下降而是成不规则增加,说明PAO1和pvdQ高表达株均能提高抗生素抗性;pvdQ高表达株对头孢他啶、环丙沙星、美罗培南和多黏菌素B几种抗生素与PAO1相比抗性提高了2～4倍.结论 铜绿假单胞菌pvdQ高表达株能够提高抗生素的抗性,说明pvdQ基因在此过程中发挥重要作用,可能通过参与群集细胞的分化来提高抗生素的抗性.
目的 探討銅綠假單胞菌pvdQ(PA2385)基因在群集遊動中對幾種常用抗生素抗性的影響.方法 構建pvdQ基因錶達質粒pME6032-pvdQ併鑒定,採用電穿孔法將帶有pvdQ基因的質粒轉入PAO1中,構建pvdQ高錶達株.同時將空質粒pME6032採用電穿孔法轉入PAO1中,構建pME6032空質粒株.在不同濃度抗生素中,通過比較群集遊動直徑的大小,觀察PAO1和pvdQ高錶達株對抗生素抗性的改變.結果 經鑒定成功構建pvdQ高錶達株,比較兩株菌群集遊動直徑大小:抗生素濃度成倍增加,但群集遊動直徑不是成倍下降而是成不規則增加,說明PAO1和pvdQ高錶達株均能提高抗生素抗性;pvdQ高錶達株對頭孢他啶、環丙沙星、美囉培南和多黏菌素B幾種抗生素與PAO1相比抗性提高瞭2～4倍.結論 銅綠假單胞菌pvdQ高錶達株能夠提高抗生素的抗性,說明pvdQ基因在此過程中髮揮重要作用,可能通過參與群集細胞的分化來提高抗生素的抗性.
목적 탐토동록가단포균pvdQ(PA2385)기인재군집유동중대궤충상용항생소항성적영향.방법 구건pvdQ기인표체질립pME6032-pvdQ병감정,채용전천공법장대유pvdQ기인적질립전입PAO1중,구건pvdQ고표체주.동시장공질립pME6032채용전천공법전입PAO1중,구건pME6032공질립주.재불동농도항생소중,통과비교군집유동직경적대소,관찰PAO1화pvdQ고표체주대항생소항성적개변.결과 경감정성공구건pvdQ고표체주,비교량주균군집유동직경대소:항생소농도성배증가,단군집유동직경불시성배하강이시성불규칙증가,설명PAO1화pvdQ고표체주균능제고항생소항성;pvdQ고표체주대두포타정、배병사성、미라배남화다점균소B궤충항생소여PAO1상비항성제고료2～4배.결론 동록가단포균pvdQ고표체주능구제고항생소적항성,설명pvdQ기인재차과정중발휘중요작용,가능통과삼여군집세포적분화래제고항생소적항성.
Objective To investigate whether Pseudomonas aeruginosa pvdQ( PA2385 ) gene reveals altered antibiotic susceptibility under swarming conditions. Methods The plasmid pME6032 with pvdQ gene was constructed and identified, then transformed into Pseudomonas aeruginosa PAO1 by the electroporation, building pvdQ overexpression strain. Using the same method building pME6032-PAO1 strain.Bacteria were inoculated in LB overnight , measuring the colony diameter of the swarming zone . Results Strains of pvdQ overexpression was successfully constructed by real-time PCR. Comparison of two strains of the swarming motility of change in diameter: The result showed that PAO1 and pvdQ overexpression strains can both improve the antibiotics resistance. Swarmer cells of pvdQ overexpression strain exhibited a 2- to 4-fold increase in antibiotic resistance toward ceftazidime,ciprofloxacin, meropenem and polymyxin B compared to PAO1 on BM2-swarming agar plates. Conclusion pvdQ gene played an important role in elevating the antibiotics resistance, which through prarticipated in the swarmer cell differentiation.
