植物学报
植物學報
식물학보
ACTA BOTANICA SINICA
2003年
3期
253-256
,共4页
cry1A基因%转基因玉米%遗传%表达%杀虫蛋白
cry1A基因%轉基因玉米%遺傳%錶達%殺蟲蛋白
cry1A기인%전기인옥미%유전%표체%살충단백
cry1A gene%transgenic maize%inheritance%expression%insecticidal protein
通过Southern杂交、ELISA分析等方法, 研究了cry1A基因在转基因玉米中的遗传与表达.结果表明,cry1A基因在转基因玉米中呈单位点显性基因遗传.cry1A杀虫蛋白在转基因玉米不同株系中的表达量存在显著差异;在转基因玉米同一植株不同组织中的表达量也明显不同,在叶片、苞叶等绿色组织中的表达量显著高于在髓、花丝等非绿色组织中的表达量;在玉米叶片中的表达量随着发育期的推进有上升的趋势;在研究的3个转基因玉米株系中,cry1A杀虫蛋白的表达量在R2、R3、R4代之间无显著差异.
通過Southern雜交、ELISA分析等方法, 研究瞭cry1A基因在轉基因玉米中的遺傳與錶達.結果錶明,cry1A基因在轉基因玉米中呈單位點顯性基因遺傳.cry1A殺蟲蛋白在轉基因玉米不同株繫中的錶達量存在顯著差異;在轉基因玉米同一植株不同組織中的錶達量也明顯不同,在葉片、苞葉等綠色組織中的錶達量顯著高于在髓、花絲等非綠色組織中的錶達量;在玉米葉片中的錶達量隨著髮育期的推進有上升的趨勢;在研究的3箇轉基因玉米株繫中,cry1A殺蟲蛋白的錶達量在R2、R3、R4代之間無顯著差異.
통과Southern잡교、ELISA분석등방법, 연구료cry1A기인재전기인옥미중적유전여표체.결과표명,cry1A기인재전기인옥미중정단위점현성기인유전.cry1A살충단백재전기인옥미불동주계중적표체량존재현저차이;재전기인옥미동일식주불동조직중적표체량야명현불동,재협편、포협등록색조직중적표체량현저고우재수、화사등비록색조직중적표체량;재옥미협편중적표체량수착발육기적추진유상승적추세;재연구적3개전기인옥미주계중,cry1A살충단백적표체량재R2、R3、R4대지간무현저차이.
We investigated the inheritance and expression of cry1A gene in transgenic maize (Zea mays L.) by Southern blotting analysis and enzyme-linked immunosorbent assays (ELISA). The results showed that cry1A had been transmitted to progeny of transgenic maize as a single gene. Contents of cry1A insecticidal protein were significantly different among transgenic maize lines and various tissues of the same transgenic lines. High expression of cry1A protein occurred in green tissues, such as leaf and husk leaf, and low expression occurred in pith, tassel, ear pith, pollen and silk. The results also showed that the contents of cry1A insecticidal protein in leaves of transgenic maize increased with the advance of development and there was no significant difference in cry1A expression level among various generations of transgenic maize.
