CAJ | 학술논문

本研究探讨重组变构人TNF相关促凋亡配体(recombinant mutant human TNF-related apootosis-inducing ligand,rmhTRAIL)联合三氧化二砷(As2O3)对阿霉素诱导的人慢性髓系白血病红白血病变耐药细胞株K562/AO2(mdr-1+)的促凋亡作用.以亲本阿霉素敏感细胞株K562(mdr-1-)为对照,观察rmhTRAIL作用后细胞形态变化,采用MTT法测定rmhTRAIL、As2O3单药及联合作用后细胞增殖抑制率;碘化丙锭染色的流式细胞术(FACSCalibur)定量检测rmhTRAILL、As2O3单药及联合作用后sub-G1峰占检测细胞的百分比(sub-G1%).结果表明:rmhTRAIL联合As2O3对K562/AO2和K562增殖的抑制作用优于单药,采用国内金(正均)氏公式判断rmhTRAIL和As2O3联合作用具有协同作用;流式细胞术定量检测sub-G1%显示,rmhTRAIL 2 000 ng/ml 与As2O3 2 μmol/L单独作用下K562/AO2组与K562组凋亡率分别为(34.93±0.10)%、(10.53±0.16)%(p＜0.01);(5.95±0.07)%、(3.50±0.01)%(p＜0.05),联合作用下细胞凋亡率分别为(50.95±0.91)%、(20.75±0.95)%(p＜0.05),K562/AO2组凋亡效应强于K562组.结论:rmhTRAIL,可诱导K562、K562/AO2细胞凋亡;rmhTRAIL联合凋亡诱导荆As2O3对白血病耐药细胞株K562/AO2和K562细胞株的凋亡具有协同作用;rmhTRAIL、As2O3对K562/AO2的促凋亡作用优于其亲本K562细胞.
본연구탐토중조변구인TNF상관촉조망배체(recombinant mutant human TNF-related apootosis-inducing ligand,rmhTRAIL)연합삼양화이신(As2O3)대아매소유도적인만성수계백혈병홍백혈병변내약세포주K562/AO2(mdr-1+)적촉조망작용.이친본아매소민감세포주K562(mdr-1-)위대조,관찰rmhTRAIL작용후세포형태변화,채용MTT법측정rmhTRAIL、As2O3단약급연합작용후세포증식억제솔;전화병정염색적류식세포술(FACSCalibur)정량검측rmhTRAILL、As2O3단약급연합작용후sub-G1봉점검측세포적백분비(sub-G1%).결과표명:rmhTRAIL연합As2O3대K562/AO2화K562증식적억제작용우우단약,채용국내금(정균)씨공식판단rmhTRAIL화As2O3연합작용구유협동작용;류식세포술정량검측sub-G1%현시,rmhTRAIL 2 000 ng/ml 여As2O3 2 μmol/L단독작용하K562/AO2조여K562조조망솔분별위(34.93±0.10)%、(10.53±0.16)%(p＜0.01);(5.95±0.07)%、(3.50±0.01)%(p＜0.05),연합작용하세포조망솔분별위(50.95±0.91)%、(20.75±0.95)%(p＜0.05),K562/AO2조조망효응강우K562조.결론:rmhTRAIL,가유도K562、K562/AO2세포조망;rmhTRAIL연합조망유도형As2O3대백혈병내약세포주K562/AO2화K562세포주적조망구유협동작용;rmhTRAIL、As2O3대K562/AO2적촉조망작용우우기친본K562세포.