CAJ | 학술논문

以人工合成的萝卜-芥蓝异源四倍体的F_4和F_(10)世代为材料,用MSAP法检测2种材料DNA甲基化变异情况,以期为远缘杂交后不同世代间基因组DNA甲基化遗传和变异规律提供实验依据.结果表明:F_4代和F_(10)代甲基化程度分别为30.61%和33.10%,共检测12种甲基化变异模式,可以分为5种类型,甲基化变异位点占所有甲基化位点的41.71%.用甲基化抑制剂5-氮胞苷处理F_4代,F_(10)代材料,检测到甲基化程度分别为20.67%和25.75%,F_(10)代比F_4代受到了更大冲击,说明不稳定世代对环境的影响更加敏感.
이인공합성적라복-개람이원사배체적F_4화F_(10)세대위재료,용MSAP법검측2충재료DNA갑기화변이정황,이기위원연잡교후불동세대간기인조DNA갑기화유전화변이규률제공실험의거.결과표명:F_4대화F_(10)대갑기화정도분별위30.61%화33.10%,공검측12충갑기화변이모식,가이분위5충류형,갑기화변이위점점소유갑기화위점적41.71%.용갑기화억제제5-담포감처리F_4대,F_(10)대재료,검측도갑기화정도분별위20.67%화25.75%,F_(10)대비F_4대수도료경대충격,설명불은정세대대배경적영향경가민감.
Methylation variations of artificial F_4 and F_(10) generations of Raphanus sativus×Brassica alboglabra allopolyploid were examined with MSAP.The results showed that the methylation levels of F_4,F_(10) were 30.61% and 33.10%,respectively.Twelve different models of methylation variations were detected,and these models could be divided into 5 types,with 41.71% variable methylation sites.The methylation levels of F_4 and F_(10) after 5-azacytidine treatment were 20.67% and 25.75%,respectively.The results showed that after 5-azacytidine treatment,methylation variations of F_(10) were stronger than those of F_4.