CAJ | 학술논문

为了筛选短短小芽孢杆菌强启动子元件,应用PCR技术从枯草杆菌168中分离出α-淀粉酶基因.用其作为报告基因与质粒pUB110和pKF3一起构建了启动子筛选载体pKB/A.将短短小芽孢杆菌细胞壁蛋白基因启动子引入该载体构建成重组质粒pKB/PA,电穿孔法转化短短小芽孢杆菌50后发现α-淀粉酶以活性形式分泌表达.结果表明短短小芽孢杆菌启动子筛选载体构建成功.
위료사선단단소아포간균강계동자원건,응용PCR기술종고초간균168중분리출α-정분매기인.용기작위보고기인여질립pUB110화pKF3일기구건료계동자사선재체pKB/A.장단단소아포간균세포벽단백기인계동자인입해재체구건성중조질립pKB/PA,전천공법전화단단소아포간균50후발현α-정분매이활성형식분비표체.결과표명단단소아포간균계동자사선재체구건성공.
To screen highly active promoters of Brevibacillus brevis, α-amylase gene as a reporter gene was isolated from Bacillus subtilis 168 by PCR, and used to construct a promoter-screening vector pKB/A with pUB110 and pKF3. The promoter of the cell wall protein gene from Br. Brevis 50 was ligated to pKB/A, producing recombinant plasmid pKB/PA. After the recombinant plasmid pKB/PA was introduced into Br. Brevis 50 by electroporation, soluble and biologically active α-amylase was secreted directly into the culture medium. The results showed that a promoter-screening vector was constructed successfully.