CAJ | 학술논문

刪除肝癌2 (DLC2), 一种经常发现在原发性肝癌过低表达的肿瘤抑制基因, 编码一种含有不育-α-基序多域蛋白质 (DLC2-SAM).以前SAM域蛋白 (DLC2-SAM) 核磁共振结构显示此蛋白是由独特的四螺旋束组成, 与其它已知SAM域结构截然不同.在该研究中, 作者运用了15N-1 H残留偶极偶合 (RDC)作为附加约束连同NOE和TALOS数据进一步优化了DLC2-SAM 的结构.由此所得的结构与没有15N-1 H残留偶极偶合约束比较显示改善了结构的质量并且有较低的Q值.螺旋的取向, 尤其是螺旋4, 被残留偶极偶合数据所验证.RDC-优化的人类DLC2-SAM的结构与小鼠的DLC2-SAM很相像.DLC家庭独特的SAM域结构表明该域可能还具有没被发现的新功能.
산제간암2 (DLC2), 일충경상발현재원발성간암과저표체적종류억제기인, 편마일충함유불육-α-기서다역단백질 (DLC2-SAM).이전SAM역단백 (DLC2-SAM) 핵자공진결구현시차단백시유독특적사라선속조성, 여기타이지SAM역결구절연불동.재해연구중, 작자운용료15N-1 H잔류우겁우합 (RDC)작위부가약속련동NOE화TALOS수거진일보우화료DLC2-SAM 적결구.유차소득적결구여몰유15N-1 H잔류우 겁우합약속비교현시개선료결구적질량 병차유교저적Q치.라선적취향, 우기시라선4, 피잔류우겁우합수거소험증.RDC-우화적인류DLC2-SAM적결구여소서적DLC2-SAM흔상상.DLC가정독특적SAM역결구표명해역가능환구유몰피발현적신공능.
The deleted in liver cancer 2 (DLC2), a tumor suppression gene which is frequently found to be underexpressed in hepatocellular carcinoma, encodes a multi-domain protein comprising a sterile α-motif domain (DLC2-SAM).Previous NMR structural studies of the SAM domain protein (DLC2-SAM) revealed a unique four helical bundle structure, distinct from any other known SAM domain structures.In the present study, we have applied 15N-1 H residual dipolar couplings as additional constraints to refining the solution structures of the DLC2-SAM together with nuclear Overhauser enhancement and TALOS data.The resulting structures show improved quality factors when comparing with the structures derived without RDC constraints and have a lower Q factor.Orientations of the helices, in particular the helix 4, are validated by residual dipolar coupling data.Our RDC-refined human DLC2-SAM structures resemble those of murine DLC2-SAM.The unique structures of the SAM domain from DLC family implicate that the SAM domain may serve novel functions although these have not yet been unveiled.