CAJ | 학술논문

背景:电针治疗膝骨关节炎具有良好的中枢和外周镇痛作用.研究显示转化生长因子β在膝骨关节炎发生中具有重要作用.目的:从转化生长因子β调控角度,认识电针治疗膝骨关节炎的可能作用机制.方法:健康3月龄雄性SD大鼠,随机分为正常组、模型组、电针组和药物组.通过结扎大鼠股静脉并强迫运动的方法,建立膝骨关节炎动物模型.造模1个月后,电针组刺激内膝眼(EX-LE4)和犊鼻(ST 35)穴位,深0.1寸,脉冲2 Hz刺激20 min,1次/d,药物组为关节腔内注射药物透明质酸钠,0.1 mL/次,1次/周.治疗2周后,采集各组动物膝关节滑膜组织,观察转化生长因子β1、转化生长因子β1受体Ⅰ、转化生长因子β受体Ⅱ的表达.结果与结论:膝关节骨关节炎动物膝关节滑膜中转化生长因子β1水平增加(P < 0.05),经电针或透明质酸钠治疗后,膝关节滑膜中转化生长因子β1水平降低(P < 0.05),且转化生长因子β1受体Ⅰ,Ⅱ含量出现显著降低(P < 0.05).提示电针治疗膝骨关节炎是通过下调转化生长因子β1的含量来改善骨关节炎症状的,受体含量的减少有助于膝骨关节炎的恢复.
배경:전침치료슬골관절염구유량호적중추화외주진통작용.연구현시전화생장인자β재슬골관절염발생중구유중요작용.목적:종전화생장인자β조공각도,인식전침치료슬골관절염적가능작용궤제.방법:건강3월령웅성SD대서,수궤분위정상조、모형조、전침조화약물조.통과결찰대서고정맥병강박운동적방법,건립슬골관절염동물모형.조모1개월후,전침조자격내슬안(EX-LE4)화독비(ST 35)혈위,심0.1촌,맥충2 Hz자격20 min,1차/d,약물조위관절강내주사약물투명질산납,0.1 mL/차,1차/주.치료2주후,채집각조동물슬관절활막조직,관찰전화생장인자β1、전화생장인자β1수체Ⅰ、전화생장인자β수체Ⅱ적표체.결과여결론:슬관절골관절염동물슬관절활막중전화생장인자β1수평증가(P < 0.05),경전침혹투명질산납치료후,슬관절활막중전화생장인자β1수평강저(P < 0.05),차전화생장인자β1수체Ⅰ,Ⅱ함량출현현저강저(P < 0.05).제시전침치료슬골관절염시통과하조전화생장인자β1적함량래개선골관절염증상적,수체함량적감소유조우슬골관절염적회복.
BACKGROUND: Electro-acupuncture therapy shows good central and peripheral analgesic effects. Several studies have shown that transforming growth factor β (TGF-β) plays an important role in the pathogenesis of knee osteoarthritis. OBJECTIVE: To investigate the mechanism underlying electro-acupuncture treatment of knee osteoarthritis. METHODS: A total of 80 healthy male 3-month-old Sprague Dawley rats were randomly and evenly divided into normal, model, electro-acupuncture and drug groups. Rat models of knee osteoarthris were estbalished by ligating the femoral veins and forcing rats to do activies. At 1 month after knee osteoarthris induction, the electro-acupuncture group rats received electro-acupuncture therapy at two acupoints Neixiyan (EX-LE4) and Dubi (ST 35) with a depth of 0.1 cun (pulse 2 Hz, 20 minutes, once a day). The drug group rats were intraarticularly administerd sodium hyaluronate (0.1 mL/administration, once a week). After 2-week treatment, synovial tissue of the knee joint was harvested to determine the exprssion of TGF-β1, TGF-β1 receptor Ⅰand TGF-β1 receptor Ⅱ. RESULTS AND CONCLUSION: TGF-β1 expression in synovial tissue of the knee joint was significantly increased after knee osteoarthris (P < 0.05), but after electro-accupuncture therepy or sodium hyaluronate treatment, TGF-β1 expression was significantly decreased (P < 0.05), moreover, TGF-β1 receptor Ⅰ, Ⅱ expression was signficantly decreased (P < 0.05). These findings suggest that electro-acupuncture for treatment of knee osteoartheis improves the symptoms of osteoarthris by downregulating TGF-β1 expression, and reduction in TGF-β1 receptor Ⅰ, Ⅱ expression promotes the recovery of knee osteoarthris.