CAJ | 학술논문

目的探讨多药耐药相关蛋白3(MRP3)在胰腺癌标本及多种肿瘤细胞中的表达及意义.方法利用免疫组织化学的方法检测30例胰腺癌与癌旁组织中MRP3的表达,同时通过RT-PCR检测7种肿瘤细胞及人胚肾293T细胞在mRNA水平的表达情况,采用抗MRP3的单克隆抗体通过流式细胞仪检测MRP3蛋白的表达.结果在各种胰腺癌标本中,MRP3在细胞核中的表达阳性细胞较正常组织明显增多(P=0.03),表达强度明显增强(P=0.015),而在胞浆上二者表达差异无统计学意义(P=0.472);MRP3 mRNA在3种人胰腺癌细胞中皆有表达,在其他5种细胞无表达,MRP3蛋白在胰腺癌细胞系BxPC-3及AsPC-1中阳性率较高(分别为68.5%和33.6%),而在PANC-1细胞中仅有5.4%表现为MRP3阳性.结论 MRP3在胰腺癌中的表达与正常胰腺组织的差别主要在细胞核,而在培养细胞中可能以组织特异性的方式表达不同,BxPC-3细胞系是体外胰腺癌耐药研究的一种选择.
목적 탐토다약내약상관단백3(MRP3)재이선암표본급다충종류세포중적표체급의의.방법 이용면역조직화학적방법검측30례이선암여암방조직중MRP3적표체,동시통과RT-PCR검측7충종류세포급인배신293T세포재mRNA수평적표체정황,채용항MRP3적단극륭항체통과류식세포의검측MRP3단백적표체.결과 재각충이선암표본중,MRP3재세포핵중적표체양성세포교정상조직명현증다(P=0.03),표체강도명현증강(P=0.015),이재포장상이자표체차이무통계학의의(P=0.472);MRP3 mRNA재3충인이선암세포중개유표체,재기타5충세포무표체,MRP3단백재이선암세포계BxPC-3급AsPC-1중양성솔교고(분별위68.5%화33.6%),이재PANC-1세포중부유5.4%표현위MRP3양성.결론 MRP3재이선암중적표체여정상이선조직적차별주요재세포핵,이재배양세포중가능이조직특이성적방식표체불동,BxPC-3세포계시체외이선암내약연구적일충선택.
Objective To investigate the expression and significance of MRP3 in pancreatic cancer and different tumor cells. Methods MRP3 expression was detected in 30 cases of pancreatic cancer tissues and the adjacent tissues through immunohistochemical method. RT-PCR was used to detect MRP3 mRNA in 7 tumor cell lines and human embryo kidney cell lines 293T. Monoclonal antibody against MRP3 was employed to detect MRP3 protein expression through flow cytometry. Results There was significant difference for MRP3 expression in nucleus between the pancreatic cancer and normal tissues beside the cancer ( P= 0.003 ) while no significant difference was found in cytoplasm (P = 0. 472). MRP3 mRNA expression was found in 3 pancreatic carcinoma cell lines. The positive expression rate of MRP3 protein was relatively high in BxPC-3 and AsPC-1 cells (68. 5% and 33.6% respectively) while it was only 5.4% in PNAC-1. Conclusions The difference of MRP3 expression in pancreatic cancer and normal tissues lies mainly in nucleus. BxPC-3 may serve as cellular models for in vitro studies on multidrug resistance of pancreatic carcinoma.