CAJ | 학술논문

目的探讨有机阴离子转运多肽1B1(OATP1B1)521T＞C与原发性高血压的相关性.方法采用实时荧光定量TaqMan-MGB探针法,检测164例原发性高血压患者和159例非高血压对照者OATP1B1 521T＞C的基因型,并将其检测结果与DNA测序结果进行比较.结果高血压组OATP1B1 521T＞C的T/C基因型频率明显低于对照组(16％、25％,P＜0.05),且OATP1B1 521T＞C的C等位基因频率明显低于对照组(10％、17％,P＜0.05),而Logistic回归分析法对影响高血压的相关因素进行回归分析,亦显示OATP1B1 521T＞C基因多态性与高血压有相关性(P＜0.05).结论 SLCO1B1的521T＞C突变为中国原发性高血压人群中的常见突变,在原发性高血压人群和血压正常人群之间的分布有差异；SLCO1B1的52IT＞C突变与原发性高血压的发病之间可能具有相关性.
목적 탐토유궤음리자전운다태1B1(OATP1B1)521T＞C여원발성고혈압적상관성.방법 채용실시형광정량TaqMan-MGB탐침법,검측164례원발성고혈압환자화159례비고혈압대조자OATP1B1 521T＞C적기인형,병장기검측결과여DNA측서결과진행비교.결과 고혈압조OATP1B1 521T＞C적T/C기인형빈솔명현저우대조조(16％、25％,P＜0.05),차OATP1B1 521T＞C적C등위기인빈솔명현저우대조조(10％、17％,P＜0.05),이Logistic회귀분석법대영향고혈압적상관인소진행회귀분석,역현시OATP1B1 521T＞C기인다태성여고혈압유상관성(P＜0.05).결론 SLCO1B1적521T＞C돌변위중국원발성고혈압인군중적상견돌변,재원발성고혈압인군화혈압정상인군지간적분포유차이；SLCO1B1적52IT＞C돌변여원발성고혈압적발병지간가능구유상관성.
Objective To study the relationship between the OATP1B1 521T ＞ C genetic polymorphism and essential hypertension.Methods 164 essential hypertension subjects and 159 normotensive subjects were detected by the TaqMan-MGB probe real-time fluorescence quantitative PCR,and the results were compared with those of DNA sequencing.Results The frequencies of T/C genotype and C allele of OATP1B1 521T ＞ C gene of the essential hypertension subjects were obviously lower than those of the normotensive subjects(T/C genotype:0.16 vs 0.25,P ＜0.05 ;C allele:0.10 vs 0.17,P ＜0.05),The difference was significant.Binary logistic stepwise regression analysis was used for evaluatine the risk factors of essential hypertension,there was significant relationship between OATP1 B1 52IT ＞ C gene polymorphism and essential hypertension.Conclusion The SLCO1 B1 521T ＞ C variant was common in Chinese essential hypertension population,but the difference of frequency of SLCO1B1 52IT ＞ Cmuton between the essential hypertension patients and the normotensive controls was of obviously statistical significance,which indicates that the SLCO1B1521T ＞ C variant maybe associate with essential hypertension.