中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
6期
757-759
,共3页
肿瘤%化疗%胰岛素%低血糖
腫瘤%化療%胰島素%低血糖
종류%화료%이도소%저혈당
Cancer%Chemotherapy%Insulin%Hypoglycemia
目的 探讨胰岛素对肿瘤细胞化疗敏感性的影响及机制.方法 以胰岛素和甲氨蝶呤(MTX)或5-氟尿嘧啶(5-Fu)处理人结肠癌细胞SW480,小鼠结肠癌细胞CT-26及小鼠乳腺癌细胞4T1,噻唑蓝(MTT)法分析细胞活力,荧光显微镜和流式细胞术观察肿瘤细胞对荧光标记MTX的摄取,并利用小鼠荷瘤模型,观察胰岛素及低MTX糖对MTX化疗疗效的影响.结果 (1)胰岛素增加MTX和5-Fu的细胞毒作用4.1%-11.5%.(2)胰岛素增加肿瘤细胞对MTX摄取141.9%.(3)胰岛素及低血糖状态增强MTX抑瘤作用20.1%.结论 胰岛素及低血糖状态增加肿瘤细胞对化疗药物的吸收,提高肿瘤细胞对化疗药物的敏感性.
目的 探討胰島素對腫瘤細胞化療敏感性的影響及機製.方法 以胰島素和甲氨蝶呤(MTX)或5-氟尿嘧啶(5-Fu)處理人結腸癌細胞SW480,小鼠結腸癌細胞CT-26及小鼠乳腺癌細胞4T1,噻唑藍(MTT)法分析細胞活力,熒光顯微鏡和流式細胞術觀察腫瘤細胞對熒光標記MTX的攝取,併利用小鼠荷瘤模型,觀察胰島素及低MTX糖對MTX化療療效的影響.結果 (1)胰島素增加MTX和5-Fu的細胞毒作用4.1%-11.5%.(2)胰島素增加腫瘤細胞對MTX攝取141.9%.(3)胰島素及低血糖狀態增彊MTX抑瘤作用20.1%.結論 胰島素及低血糖狀態增加腫瘤細胞對化療藥物的吸收,提高腫瘤細胞對化療藥物的敏感性.
목적 탐토이도소대종류세포화료민감성적영향급궤제.방법 이이도소화갑안접령(MTX)혹5-불뇨밀정(5-Fu)처리인결장암세포SW480,소서결장암세포CT-26급소서유선암세포4T1,새서람(MTT)법분석세포활력,형광현미경화류식세포술관찰종류세포대형광표기MTX적섭취,병이용소서하류모형,관찰이도소급저MTX당대MTX화료료효적영향.결과 (1)이도소증가MTX화5-Fu적세포독작용4.1%-11.5%.(2)이도소증가종류세포대MTX섭취141.9%.(3)이도소급저혈당상태증강MTX억류작용20.1%.결론 이도소급저혈당상태증가종류세포대화료약물적흡수,제고종류세포대화료약물적민감성.
Objective To investigate the effect and mechanism of insulin and hypoglycemia on the chemosensitivity of cancer cells. Methods Human colon cancer cells SW480, mouse colon cancer cells CT-26 and mouse breast cancer cells 4T1 were cultured and treated with insulin and chemotheraputic drugs, 5-fluorouracil or methotrexate. MTT assay was used to measure the cytotoxity. Fluorescence microscopy and flow cytometry were used to determine the effects of insulin on the uptake of fluorescent methotrexate by cancer cells. Mouse breast cancer cells 4T1 were inoculated into syngeneic BALB/C mice. Insulin was administrated to the mice before giving methotrexate and the therapeutic effect was monitored. Results (1) Insulin enhanced the chemocytotoxity of chemotheraputic drugs on cancer cells by 4. 1% to 11.5%;(2) Insulin increased the uptake of fluorescent methotrexate by cancer cells in vitro by 141. 9% ;(3) Insulin and hypoglycemia enhanced the chemotherapeutic effect of methotrexate on mouse breast cancer models by 20. 1%. Conclusion Insulin and hypoglycemia increase absorption of chemotherapeutic agents by cancer cells and enhance the chemosensitivity of cancer cells.
